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Correction to Int. J. Mol. Sci. 2024, 25(4), 2388.
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Correction

Correction: Kim et al. The Auto-Regulation of ATL2 E3 Ubiquitin Ligase Plays an Important Role in the Immune Response against Alternaria brassicicola in Arabidopsis thaliana. Int. J. Mol. Sci. 2024, 25, 2388

by
Daewon Kim
1,2,†,
Su Jeong Jeon
1,†,
Jeum Kyu Hong
3,4,
Min Gab Kim
5,
Sang Hee Kim
1,
Ulhas S. Kadam
1,
Woe-Yeon Kim
6,
Woo Sik Chung
1,
Gary Stacey
2 and
Jong Chan Hong
1,2,*
1
Division of Life Science and Division of Applied Life Science (BK21 Four), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Republic of Korea
2
Division of Plant Science & Technology, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
3
Laboratory of Horticultural Crop Protection, Division of Horticultural Science, Gyeongsang National University, 33 Dongjin-ro, Jinju 52725, Republic of Korea
4
Agri-Food Bio Convergence Institute, Gyeongsang National University, 33 Dongjin-ro, Jinju 52725, Republic of Korea
5
College of Pharmacy and Research Institute of Pharmaceutical Science, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Republic of Korea
6
Division of Applied Life Science (BK21 Four), Plant Biological Rhythm Research Center (PBRRC), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, 501 Jinju-daero, Jinju 52828, Republic of Korea
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(18), 10095; https://doi.org/10.3390/ijms251810095
Submission received: 2 August 2024 / Accepted: 15 August 2024 / Published: 20 September 2024
(This article belongs to the Section Molecular Plant Sciences)
Browse Figure
Versions Notes
In the original publication [1], there was a mistake in Figure 4 as published. The Col-0 image in Figure 4C was mistakenly duplicated as the ATL2C138AOX7-5 image. The corrected Figure 4 appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Reference

  1. Kim, D.; Jeon, S.J.; Hong, J.K.; Kim, M.G.; Kim, S.H.; Kadam, U.S.; Kim, W.-Y.; Chung, W.S.; Stacey, G.; Hong, J.C. The Auto-Regulation of ATL2 E3 Ubiquitin Ligase Plays an Important Role in the Immune Response against Alternaria brassicicola in Arabidopsis thaliana. Int. J. Mol. Sci. 2024, 25, 2388. [Google Scholar] [CrossRef]
Ijms 25 10095 g004
Figure 4. Requirement of ATL2 E3 ligase activity for disease response to A. brassicicola. (A) The activity of ATL2 E3 is dependent on the presence of the Cys138 residue in the RING domain. To analyze self-ubiquitination assays of MBP-ATL2 or MBP-ATL2C138A proteins in the presence of rabbit E1, human E2, and Ub, Western blot analyses were conducted using anti-MBP and anti-Ub antibodies. Arrows denote the non-ubiquitinated forms of the proteins. (B) RT-PCR analysis of ATL2 transcripts of Col-0, ATL2C138AOX2-1-, and ATL2C138AOX7-5-overexpressing plants. ACTIN2 was used as a loading control. (C) The presence of the C138A mutation in the RING domain of ATL2 is necessary for a disease response to A. brassicicola. Photographs of the leaves of Col-0, ATL2OX4-1-, ATL2C138AOX2-1-, and ATL2C138AOX7-5-overexpressing plants post-infection with A. brassicicola are shown. Detached leaves from 6-week-old plants were inoculated with a 5 µL droplet of A. brassicicola spores (approximately 5 × 105 spores/mL), and photographs were taken 6 days after inoculation. The white arrows indicate inoculated regions. (D) Size of lesions in A. brassicicola infected Col-0, ATL2OX4-1-, ATL2C138AOX2-1-, and ATL2C138AOX7-5-overexpressing plant leaves. Lesion size was measured at 6 days after inoculation. (E) Trypan blue staining showing fungal hyphae of A. brassicicola on Arabidopsis leaves; Col-0, ATL2OX4-1, ATL2C138AOX2-1, and ATL2C138AOX7-5. Scale bars = 50 µm. (F) Measurement of spore numbers in lesions. The spore numbers in lesions were measured by collecting total spores from detached inoculated leaves and counting them microscopically at 6 days after inoculation. The data are shown as mean ± SD (n = 3), with ** p < 0.005, and *** p < 0.001 indicating the significance level. In panels D and F, the p-value indicates the significance relative to the A. brassicicola treatment at wild-type (Col-0) plants and was determined and analyzed using GraphPad Prism 8 via two-way ANOVA followed by Dunnett’s multiple comparison. n.s represents not significant. These experiments were repeated three times (biological replicates) with similar results.
Figure 4. Requirement of ATL2 E3 ligase activity for disease response to A. brassicicola. (A) The activity of ATL2 E3 is dependent on the presence of the Cys138 residue in the RING domain. To analyze self-ubiquitination assays of MBP-ATL2 or MBP-ATL2C138A proteins in the presence of rabbit E1, human E2, and Ub, Western blot analyses were conducted using anti-MBP and anti-Ub antibodies. Arrows denote the non-ubiquitinated forms of the proteins. (B) RT-PCR analysis of ATL2 transcripts of Col-0, ATL2C138AOX2-1-, and ATL2C138AOX7-5-overexpressing plants. ACTIN2 was used as a loading control. (C) The presence of the C138A mutation in the RING domain of ATL2 is necessary for a disease response to A. brassicicola. Photographs of the leaves of Col-0, ATL2OX4-1-, ATL2C138AOX2-1-, and ATL2C138AOX7-5-overexpressing plants post-infection with A. brassicicola are shown. Detached leaves from 6-week-old plants were inoculated with a 5 µL droplet of A. brassicicola spores (approximately 5 × 105 spores/mL), and photographs were taken 6 days after inoculation. The white arrows indicate inoculated regions. (D) Size of lesions in A. brassicicola infected Col-0, ATL2OX4-1-, ATL2C138AOX2-1-, and ATL2C138AOX7-5-overexpressing plant leaves. Lesion size was measured at 6 days after inoculation. (E) Trypan blue staining showing fungal hyphae of A. brassicicola on Arabidopsis leaves; Col-0, ATL2OX4-1, ATL2C138AOX2-1, and ATL2C138AOX7-5. Scale bars = 50 µm. (F) Measurement of spore numbers in lesions. The spore numbers in lesions were measured by collecting total spores from detached inoculated leaves and counting them microscopically at 6 days after inoculation. The data are shown as mean ± SD (n = 3), with ** p < 0.005, and *** p < 0.001 indicating the significance level. In panels D and F, the p-value indicates the significance relative to the A. brassicicola treatment at wild-type (Col-0) plants and was determined and analyzed using GraphPad Prism 8 via two-way ANOVA followed by Dunnett’s multiple comparison. n.s represents not significant. These experiments were repeated three times (biological replicates) with similar results.
Ijms 25 10095 g004
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MDPI and ACS Style

Kim, D.; Jeon, S.J.; Hong, J.K.; Kim, M.G.; Kim, S.H.; Kadam, U.S.; Kim, W.-Y.; Chung, W.S.; Stacey, G.; Hong, J.C. Correction: Kim et al. The Auto-Regulation of ATL2 E3 Ubiquitin Ligase Plays an Important Role in the Immune Response against Alternaria brassicicola in Arabidopsis thaliana. Int. J. Mol. Sci. 2024, 25, 2388. Int. J. Mol. Sci. 2024, 25, 10095. https://doi.org/10.3390/ijms251810095

AMA Style

Kim D, Jeon SJ, Hong JK, Kim MG, Kim SH, Kadam US, Kim W-Y, Chung WS, Stacey G, Hong JC. Correction: Kim et al. The Auto-Regulation of ATL2 E3 Ubiquitin Ligase Plays an Important Role in the Immune Response against Alternaria brassicicola in Arabidopsis thaliana. Int. J. Mol. Sci. 2024, 25, 2388. International Journal of Molecular Sciences. 2024; 25(18):10095. https://doi.org/10.3390/ijms251810095

Chicago/Turabian Style

Kim, Daewon, Su Jeong Jeon, Jeum Kyu Hong, Min Gab Kim, Sang Hee Kim, Ulhas S. Kadam, Woe-Yeon Kim, Woo Sik Chung, Gary Stacey, and Jong Chan Hong. 2024. "Correction: Kim et al. The Auto-Regulation of ATL2 E3 Ubiquitin Ligase Plays an Important Role in the Immune Response against Alternaria brassicicola in Arabidopsis thaliana. Int. J. Mol. Sci. 2024, 25, 2388" International Journal of Molecular Sciences 25, no. 18: 10095. https://doi.org/10.3390/ijms251810095

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