IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β-Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells
Abstract
:1. Introduction
2. Results
2.1. β-cyc Exhibits NGF-Mimic and NGF-Enhancing Effects on PC12 Cells
2.2. Ras/Raf/MEK/ERK Signaling Pathway Involves in NGF-Mimic and NGF–Enhancing Effect of β-cyc
2.3. IGF-1R/PI3K/AKT Signaling Pathway Takes an Important Role in NGF-Mimic Effect of β-cyc
2.4. GR/PLC/PKC Signaling Pathway Participates in the NGF-Mimic Effect of β-cyc
2.5. β-cyc Increases the Thermal Stable Ability of IGF-1R in CETSA
2.6. β-cyc Enhances the Stability of IGF-1R and GR Proteins for Pronase E During DARTS Analysis
3. Discussion
4. Materials and Methods
4.1. Antibodies and Reagents
4.2. Extraction and Isolation
4.3. Evaluation of the Neuritogenic Activity of PC12 Cells
4.4. Cell Viability by MTT Bioassay
4.5. Western Blot Analysis
4.6. CETSA
4.7. DARTS
4.8. Quantification and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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An, C.; Gao, L.; Xiang, L.; Qi, J. IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β-Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells. Int. J. Mol. Sci. 2024, 25, 9763. https://doi.org/10.3390/ijms25189763
An C, Gao L, Xiang L, Qi J. IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β-Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells. International Journal of Molecular Sciences. 2024; 25(18):9763. https://doi.org/10.3390/ijms25189763
Chicago/Turabian StyleAn, Chenyue, Lijuan Gao, Lan Xiang, and Jianhua Qi. 2024. "IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β-Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells" International Journal of Molecular Sciences 25, no. 18: 9763. https://doi.org/10.3390/ijms25189763