The Role of the Mu Opioid Receptors of the Medial Prefrontal Cortex in the Modulation of Analgesia Induced by Acute Restraint Stress in Male Mice
Abstract
:1. Introduction
2. Results
2.1. Acute Restraint Stress Produces mPFC MOR-Dependent SIA
2.2. GABAergic but Not Glutamatergic MORs in the mPFC Modulate MOR-Dependent SIA
2.3. GABAergic MORs in the mPFC Modulate the Activity of mPFC–PAG Projections under the MOR-Dependent SIA Paradigm
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Restraint Stress
4.3. Pain Behavioral Test
4.4. Surgery and Adeno-Associated Virus Injection
4.5. Fluorescence In Situ Hybridization Using an RNAscope
4.6. C-Fos Immunofluorescence
4.7. Intra-mPFC Injection
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Du, Y.; Zhao, Y.; Zhang, A.; Li, Z.; Wei, C.; Zheng, Q.; Qiao, Y.; Liu, Y.; Ren, W.; Han, J.; et al. The Role of the Mu Opioid Receptors of the Medial Prefrontal Cortex in the Modulation of Analgesia Induced by Acute Restraint Stress in Male Mice. Int. J. Mol. Sci. 2024, 25, 9774. https://doi.org/10.3390/ijms25189774
Du Y, Zhao Y, Zhang A, Li Z, Wei C, Zheng Q, Qiao Y, Liu Y, Ren W, Han J, et al. The Role of the Mu Opioid Receptors of the Medial Prefrontal Cortex in the Modulation of Analgesia Induced by Acute Restraint Stress in Male Mice. International Journal of Molecular Sciences. 2024; 25(18):9774. https://doi.org/10.3390/ijms25189774
Chicago/Turabian StyleDu, Yinan, Yukui Zhao, Aozhuo Zhang, Zhiwei Li, Chunling Wei, Qiaohua Zheng, Yanning Qiao, Yihui Liu, Wei Ren, Jing Han, and et al. 2024. "The Role of the Mu Opioid Receptors of the Medial Prefrontal Cortex in the Modulation of Analgesia Induced by Acute Restraint Stress in Male Mice" International Journal of Molecular Sciences 25, no. 18: 9774. https://doi.org/10.3390/ijms25189774