Composite Polycaprolactone/Gelatin Nanofiber Membrane Scaffolds for Mesothelial Cell Culture and Delivery in Mesothelium Repair
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of PCL and PCL/Gelatin Nanofiber Membrane Scaffolds (NMSs)
2.2. In Vitro Study
2.3. In Vivo Study
3. Materials and Methods
3.1. Materials
3.2. Preparation of Electrospun Nanofiber Membrane Scaffolds (NMSs)
3.3. Characterization of Electrospun Nanofiber Membrane Scaffolds (NMSs)
3.4. In Vitro Cell Culture
3.5. Cell Morphology by Scanning Electron Microscope (SEM) Observation
3.6. Cell Proliferation by DNA Contents and MTT Assays
3.7. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
3.8. Cell Viability by Live/Dead Staining
3.9. Cytoskeleton Staining
3.10. Immunofluorescence (IF) Staining
3.11. In Vivo Studies
3.12. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Scaffolds | Maximum Load (N) | Maximum Displacement (mm) | Stiffness (N/mm) |
---|---|---|---|
PCL (dry) | 8.56 ± 0.95 | 25.00 ± 3.63 | 1.26 ± 0.06 |
PCL/gelatin (dry) | 9.19 ± 0.41 | 14.94 ± 1.18 α | 3.72 ± 0.46 α |
PCL (wet) | 9.44 ± 0.31 | 11.46 ± 1.86 | 1.96 ± 0.33 |
PCL/gelatin (wet) | 10.07 ± 0.39 | 5.31 ± 0.89 β | 3.10 ± 0.32 β |
Culture Time (Days) | Calretinin (%) | E-Cadherin (%) | ||
---|---|---|---|---|
PCL | PCL/Gelatin | PCL | PCL/Gelatin | |
1 | 10.68 ± 0.57 | 23.13 ± 1.00 * | 10.26 ± 0.35 | 28.10 ± 0.16 * |
3 | 20.10 ± 0.30 | 49.88 ± 0.43 * | 21.20 ± 0.36 | 36.82 ± 0.35 * |
5 | 38.82 ± 0.33 | 80.20 ± 0.54 * | 38.10 ± 0.20 | 66.20 ± 1.84 * |
7 | 41.33 ± 1.00 | 88.10 ± 0.20 * | 48.30 ± 1.45 | 84.70 ± 0.21 * |
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Govindaraju, D.T.; Kao, H.-H.; Chien, Y.-M.; Chen, J.-P. Composite Polycaprolactone/Gelatin Nanofiber Membrane Scaffolds for Mesothelial Cell Culture and Delivery in Mesothelium Repair. Int. J. Mol. Sci. 2024, 25, 9803. https://doi.org/10.3390/ijms25189803
Govindaraju DT, Kao H-H, Chien Y-M, Chen J-P. Composite Polycaprolactone/Gelatin Nanofiber Membrane Scaffolds for Mesothelial Cell Culture and Delivery in Mesothelium Repair. International Journal of Molecular Sciences. 2024; 25(18):9803. https://doi.org/10.3390/ijms25189803
Chicago/Turabian StyleGovindaraju, Darshan Tagadur, Hao-Hsi Kao, Yen-Miao Chien, and Jyh-Ping Chen. 2024. "Composite Polycaprolactone/Gelatin Nanofiber Membrane Scaffolds for Mesothelial Cell Culture and Delivery in Mesothelium Repair" International Journal of Molecular Sciences 25, no. 18: 9803. https://doi.org/10.3390/ijms25189803