Combining rVAR2 and Anti-EpCAM to Increase the Capture Efficiency of Non-Small-Cell Lung Cancer Cell Lines in the Flow Enrichment Target Capture Halbach (FETCH) Magnetic Separation System
Abstract
:1. Introduction
2. Results
2.1. Comparison of ofCS, EpCAM, PD-L1, and EGFR Expression on NSCLC Cell Lines
2.2. Effect of Cell Fixation on ofCS Expression in NSCLC Cell Lines
2.3. Comparison of Capture Efficiency in PBS, Whole Blood, and Lysed Blood in NSCLC Cell Line
2.4. Comparison of the Capture Efficiency Using Anti-EpCAM Antibody and rVAR2 in Lysed Blood
2.5. Capture Efficiency When Using a Combination of Anti-EpCAM Antibody and rVAR2 Protein in Spiked Lysed Blood
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and Blood Sample Collection
4.2. Optimization of the Concentration of rVAR2 and Characterization of ofCS Expression in NSCLC Cell Lines
4.3. Characterization of EpCAM, PD-L1, and EGFR Expression in NSCLC Cell Lines
4.4. Effect of Fixative on ofCS Expression in NSCLC Cell Lines
4.5. Comparison of Capture Experiments in PBS, Lysed Blood, and Whole Blood
4.6. Comparison of the Capture Efficiency of Anti-EpCAM Antibody and SpyCatcher-rVAR2 or Their Combination in Spiked Lysed Blood
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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He, S.; Liu, P.; Wu, Y.; Agerbæk, M.Ø.; Salanti, A.; Terstappen, L.W.M.M.; Jonkheijm, P.; Stevens, M. Combining rVAR2 and Anti-EpCAM to Increase the Capture Efficiency of Non-Small-Cell Lung Cancer Cell Lines in the Flow Enrichment Target Capture Halbach (FETCH) Magnetic Separation System. Int. J. Mol. Sci. 2024, 25, 9816. https://doi.org/10.3390/ijms25189816
He S, Liu P, Wu Y, Agerbæk MØ, Salanti A, Terstappen LWMM, Jonkheijm P, Stevens M. Combining rVAR2 and Anti-EpCAM to Increase the Capture Efficiency of Non-Small-Cell Lung Cancer Cell Lines in the Flow Enrichment Target Capture Halbach (FETCH) Magnetic Separation System. International Journal of Molecular Sciences. 2024; 25(18):9816. https://doi.org/10.3390/ijms25189816
Chicago/Turabian StyleHe, Sitian, Peng Liu, Yongjun Wu, Mette Ø. Agerbæk, Ali Salanti, Leon W. M. M. Terstappen, Pascal Jonkheijm, and Michiel Stevens. 2024. "Combining rVAR2 and Anti-EpCAM to Increase the Capture Efficiency of Non-Small-Cell Lung Cancer Cell Lines in the Flow Enrichment Target Capture Halbach (FETCH) Magnetic Separation System" International Journal of Molecular Sciences 25, no. 18: 9816. https://doi.org/10.3390/ijms25189816