The Interplay between Oxidative Stress and Sphingolipid Metabolism in Endometrial Cancer
Abstract
:1. Introduction
2. Results
2.1. Characteristics of the Studied Groups
2.2. Sphingolipids
2.3. Redox Homeostasis and Lipid Peroxidation
2.4. Correlations between Oxidative Status and Lipid Concentration
3. Discussion
4. Materials and Methods
4.1. Study Participants
4.2. Sample Collection
4.3. Laboratory Assessments
4.3.1. Sphingolipids Measurement
4.3.2. Oxidative Status
4.3.3. Lipid Peroxidation
4.4. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Control | EC | ||||
---|---|---|---|---|---|
n | 17 | 43 | |||
Age (years) | 59 (36–82) | 64 (39–86) | |||
Weight | 80 (72–116) | 83 (50–139) | |||
BMI (kg/m2) | 29.76 (20.9–39.21) | 32.23 (21.64–54.98) | |||
Fasting glucose (mg/dL) | 108.13 (81–143) | 116.97 (84–231) | |||
Glucose 30′ (mg/dL) | 167.23 (82–262) | 185.93 (139–307) | |||
Glucose 60′ (mg/dL) | 160.15 (110–221) | 202.15 (108–356) * | |||
Glucose 120′ (mg/dL) | 123.61 (44–185) | 174.51 (78–340) ** | |||
HOMA–IR | 8.89 (0.93–18.07) | 18.30 (0.96–48.87) | |||
Menopause | Premenopausal | 50 (45–56) | 47.25 (40–54) | (44–57) ** | - * |
Fertility | 2 (0–4) | 3(0–8) | |||
Total cholesterol (mmol/L) | 208.8 (138–302) | 176.23 (153–213) * | |||
HDL-cholesterol (mmol/L) | 60.40 (34.41–106.93) | 56.82 (26.62–83.74) | |||
LDL-cholesterol (mmol/L) | 128.90 (56.6–217.8) | 108.72 (73.2–152.3) | |||
Triglycerides (mmol/L) | 145.15 (60–280) | 122 (41–267) | |||
MDA (mmol/mg) | 1.96 (1.88–2.00) | 3.75 (3.56–3.92) **** |
Control | EC | p-Value | |
---|---|---|---|
Me (Q1–Q3) [pmol/mg] | Me (Q1–Q3) [pmol/mg] | ||
Sph | 1.67 (1.37–2.34) | 5.49 (4.97–6.09) **** | <0.0001 |
SPA | 0.48 (0.39–0.71) | 2.14 (1.79–2.46) **** | <0.0001 |
S1P | 0.15 (0.13–0.16) | 0.21 (0.17–0.31) **** | <0.0001 |
C14:0 Cer | 0.17 (0.21–0.13) | 0.92 (0.81–1.09) **** | <0.0001 |
C16:0 Cer | 14.85 (12.28–17.34) | 46.32 (36.17–54.42) **** | <0.0001 |
C18:1 Cer | 0.08 (0.07–0.09) | 0.26 (0.23–0.41) **** | <0.0001 |
C18:0 Cer | 3.20 (2.39–3.39) | 4.76 (4.07–5.83) **** | <0.0001 |
C20:0 Cer | 0.65 (0.56–0.72) | 1.10 (0.90–1.41) **** | <0.0001 |
C22:0 Cer | 2.62 (1.94–3.07) | 5.20 (4.30–6.03) **** | <0.0001 |
C24:1 Cer | 9.05 (8.68–13.96) | 14.96 (13.30–16.77) **** | <0.0001 |
C24:0 Cer | 6.30 (5.56–7.03) | 11.71 (9.74–12.93) **** | <0.0001 |
Cer Total | 36.99 (32.22–44.63) | 82.69 (70.58–96.02) **** | <0.0001 |
Control | EC | |
---|---|---|
Me (Q1–Q3) [pmol/mL] | Me (Q1–Q3) [pmol/mL] | |
Sph | 6.01 (5.02–7.51) | 6.36 (4.88–8.12) |
SPA | 3.54 (2.97–5.26) | 4.18 (3.29–5.69) |
S1P | 690.74 (622.36–812.05) | 719.0 (605.8–798.5) |
C14:0 Cer | 17.40 (14.77–21.47) | 18.42 (16.52–20.23) |
C16:0 Cer | 198.3 (165.1–259.6) | 186.8 (156.6–225.4) |
C18:1 Cer | 9.82 (8.99–10.96) | 10.83 (8.65–14.40) |
C18:0 Cer | 98.1 (84.2–123.7) | 95.6 (80.5–124.2) |
C20:0 Cer | 130.1 (95.7–177.6) | 135.5 (114.7–165.6) |
C22:0 Cer | 496.8 (343.4–549.4) | 429.2 (338.4–496.3) |
C24:1 Cer | 934.3 (666.5–1050.1) | 837.5 (700.1–1144.2) |
C24:0 Cer | 2506.2 (2234.5–3269.0) | 2644.3 (1894.4–2915.1) |
Cer Total | 4510.9 (3787.9–5227.0) | 4426.6 (3580.2–4830.8) |
TOS | TAC | OSI | |
---|---|---|---|
Sph | r = 0.5074 p = 0.0135 * | r = −0.0352 p = 0.8733 | r = 0.3999 p = 0.0587 |
SPA | r = 0.5517 p = 0.0064 ** | r = −0.1447 p = 0.5101 | r = 0.5427 p = 0.0075 ** |
S1P | r = 0.1732 p = 0.4295 | r = −0.2284 p = 0.2945 | r = 0.2100 p = 0.3361 |
C14:0 Cer | r = 0.4557 p = 0.0289 * | r = −0.1489 p = 0.4976 | r = 0.4257 p = 0.0428 * |
C16:0 Cer | r = 0.5212 p = 0.0108 * | r = −0.1630 p = 0.4573 | r = 0.4935 p = 0.0167 * |
C18:1 Cer | r = 0.4139 p = 0.0496 * | r = −0,1216 p = 0.5805 | r = 0.3799 p = 0.0738 |
C18:0 Cer | r = 0.3102 p = 0.1498 | r = −0.1820 p = 0.4058 | r = 0.3582 p = 0.0933 |
C20:0 Cer | r = 0.5010 p = 0.0149 * | r = 0.0736 p = 0.7386 | r = 0.2845 p = 0.1883 |
C22:0 Cer | r = 0.6415 p = 0.0010 *** | r = −0.1616 p = 0.4612 | r = 0.5822 p = 0.0036 ** |
C24:1 Cer | r = 0.2332 p = 0.2843 | r = −0.2166 p = 0.3208 | r = 0.4353 p = 0.0379 * |
C24:0 Cer | r = 0.5518 p = 0.0063 ** | r = −0.1467 p = 0.5042 | r = 0.5084 p = 0.0132 * |
Total Cer | r = 0.5214 p = 0.0107 * | p = 0.4194 r = −0.1769 | r = 0.5226 p = 0.0105 * |
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Błachnio-Zabielska, A.U.; Sadowska, P.; Zdrodowski, M.; Laudański, P.; Szamatowicz, J.; Kuźmicki, M. The Interplay between Oxidative Stress and Sphingolipid Metabolism in Endometrial Cancer. Int. J. Mol. Sci. 2024, 25, 10243. https://doi.org/10.3390/ijms251910243
Błachnio-Zabielska AU, Sadowska P, Zdrodowski M, Laudański P, Szamatowicz J, Kuźmicki M. The Interplay between Oxidative Stress and Sphingolipid Metabolism in Endometrial Cancer. International Journal of Molecular Sciences. 2024; 25(19):10243. https://doi.org/10.3390/ijms251910243
Chicago/Turabian StyleBłachnio-Zabielska, Agnieszka U., Patrycja Sadowska, Michał Zdrodowski, Piotr Laudański, Jacek Szamatowicz, and Mariusz Kuźmicki. 2024. "The Interplay between Oxidative Stress and Sphingolipid Metabolism in Endometrial Cancer" International Journal of Molecular Sciences 25, no. 19: 10243. https://doi.org/10.3390/ijms251910243