Mica Lattice Orientation of Epitaxially Grown Amyloid β25–35 Fibrils

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

please see attachment

Comments for author File: Comments.pdf

Comments on the Quality of English Language

n/a

Author Response

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Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

In this work, Ferenczy and coworkers describe the different orientations of Aβ(25-35) on mica lattice using AFM and MD simulations to predict their molecular behaviour.

The results are potentially interesting for their application in the biotechnological field. 

My only concern is about the reliability of the simulations. As the authors may be aware, short MD simulations may underestimate some critical events; therefore, to make these results even more appealing, I would suggest performing two additional 100 ns replicas for each simulation to have an idea of their reproducibility.

Comments on the Quality of English Language

An extensive review of the English Language should be applied. Moreover, the character used for "β" in the paper is wrong. It should be replaced with the one reported in line 103, which is the correct one.

Author Response

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Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

Referee report on the paper “Mica lattice orientation of epitaxially grown amyloid Abeta 25-35 fibrils by György G. Ferenczy, Ünige Murvai, Lívia Fülöp and Miklós Kellermayer - ijms-3214734

By using high-resolution atomic force microscopy, the Authors are able to elucidate the arrangement of Abeta25-35 fibrils on a mica lattice. To validate the structure and test the stability of the oriented fibrils, molecular dynamics simulations on beta-sheets models were carried out.

The paper contains sufficiently interesting structural information to deserve publication in ijms.

Comments on the Quality of English Language

English is fine

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Comments and Suggestions for Authors

Thank you for following my suggestions.