S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer
Abstract
:1. Introduction
2. Results
2.1. MRN Complex Signaling Is Diminished in Endogenously Radioresistant Cells
2.2. S6K1 Inhibition Sensitizes Lung Cancer Cells to Rradiation
2.3. S6K1 Modulates DNA Damage Signaling by Regulating Activation of MRN Complex Members
2.4. S6K1 Overexpression Increases Resistance to Radiation and Impairs Activation of MRN Signaling
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Western Blot
4.3. Colony Formation Assay
4.4. Crispr-Cas9 Cells
4.5. Cell Transfection
4.6. Proliferation Assays
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Calderon-Aparicio, A.; He, J.; Simone, N.L. S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer. Int. J. Mol. Sci. 2024, 25, 10461. https://doi.org/10.3390/ijms251910461
Calderon-Aparicio A, He J, Simone NL. S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer. International Journal of Molecular Sciences. 2024; 25(19):10461. https://doi.org/10.3390/ijms251910461
Chicago/Turabian StyleCalderon-Aparicio, Ali, Jun He, and Nicole L. Simone. 2024. "S6K1 Controls DNA Damage Signaling Modulated by the MRN Complex to Induce Radioresistance in Lung Cancer" International Journal of Molecular Sciences 25, no. 19: 10461. https://doi.org/10.3390/ijms251910461