Pterostilbene Induces Pyroptosis in Breast Cancer Cells through Pyruvate Kinase 2/Caspase-8/Gasdermin C Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. PTE-Induced Pyroptosis in EMT6 and 4T1 Cells
2.2. PTE-Induced Cancer Cell Pyroptosis Is Mediated by GSDMC
2.3. PTE Inhibits the Glycolysis of EMT6 and 4T1 Cells
2.4. PTE Inhibits Glycolysis by Regulating PKM2, a Key Rate-Limiting Enzyme in Glycolysis
2.5. PTE-Activated Caspase-8/GSDMC Cascade through Repressing PKM2
2.6. PTE Reduces Tumorigenicity in Mouse Breast Tumor Models
3. Discussion
4. Methods
4.1. Cell Culture
4.2. Test Animals
4.3. Preparation Solutions
4.4. Cell Counting Kit-8 (CCK8) Assays
4.5. Morphological Observations
4.6. Real-Time Fluorescence Quantitative PCR (qPCR)
4.7. Western Blot (WB)
4.8. Immunofluorescence
4.9. DSS Cross-Linking Test
4.10. Transfection
4.11. Nucleoplasmic Separation
4.12. Pyruvate Kinase Activity
4.13. Glucose, Lactate, and Adenosine Triphosphate (ATP) Detection
4.14. Co-IP
4.15. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
adenosine triphosphate | ATP |
Cell Counting Kit-8 | CCK8 |
Dimethyl sulfoxide | DMSO |
Gasdermins | GSDMs |
hexokinase 2 | HK2 |
interleukin | IL |
Lactate dehydrogenase | LDH |
Pterostilbene | PTE |
phosphofructokinase-1 | PFK-1 |
phosphate-buffered saline | PBS, pH 8.0 |
pyruvate kinase | PK |
standard deviation | SD |
Western Blot | WB |
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Pan, T.; Peng, L.; Dong, J.; Li, L. Pterostilbene Induces Pyroptosis in Breast Cancer Cells through Pyruvate Kinase 2/Caspase-8/Gasdermin C Signaling Pathway. Int. J. Mol. Sci. 2024, 25, 10509. https://doi.org/10.3390/ijms251910509
Pan T, Peng L, Dong J, Li L. Pterostilbene Induces Pyroptosis in Breast Cancer Cells through Pyruvate Kinase 2/Caspase-8/Gasdermin C Signaling Pathway. International Journal of Molecular Sciences. 2024; 25(19):10509. https://doi.org/10.3390/ijms251910509
Chicago/Turabian StylePan, Tingting, Li Peng, Jing Dong, and Lin Li. 2024. "Pterostilbene Induces Pyroptosis in Breast Cancer Cells through Pyruvate Kinase 2/Caspase-8/Gasdermin C Signaling Pathway" International Journal of Molecular Sciences 25, no. 19: 10509. https://doi.org/10.3390/ijms251910509