Preliminary Investigation of Potential Early Biomarkers for Gestational Diabetes Mellitus: Insights from PTRPG and IGKV2D-28 Expression Analysis
Abstract
:1. Introduction
2. Results
2.1. Increased PTPRG and IGKV2D-28 Expression Levels in the GDM and Non-GDM Groups
2.2. Correlations between Gene Expressions with Clinical Variables and GDM Status
2.3. Diagnostic Potential of PTPRG and IGKV2D-28 in GDM Prediction
3. Discussion
4. Materials and Methods
4.1. First-Trimester RNA Samples
4.2. Reverse Transcription and cDNA Synthesis
4.3. Real-Time Quantitative RT-PCR
4.4. Computation of Gene Expression
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Candidate Genes | Glucose | 1st Hour OGTT | 2nd Hour OGTT | HbA1c | GDM Status | |||||
---|---|---|---|---|---|---|---|---|---|---|
r | p Value | r | p Value | r | p Value | r | p Value | r | p Value | |
PTPRG (2−ΔΔCT) | 0.40 b | 0.01 a | −0.02 b | 0.88 | −0.21 b | 0.17 | 0.18 b | 0.23 | 0.31 b | 0.03 a |
IGKV2D-28 (2−ΔΔCT) | 0.34 b | 0.01 a | −0.08 b | 0.57 | −0.14 b | 0.34 | 0.21 b | 0.15 | 0.32 b | 0.01 a |
Biochemical Characteristics | Non-GDM (n = 36) | GDM (n = 24) |
---|---|---|
Glucose (mmol/L) | 4.49 ± 0.39 | 5.51 ± 0.78 |
First hour OGTT (mmol/L) | 6.29 ± 0.83 | 8.55 ± 1.75 |
Second hour OGTT (mmol/L) | 6.28 ± 0.98 | 7.69 ± 1.88 |
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Payot, M.D.; Villavieja, A.; Pineda-Cortel, M.R. Preliminary Investigation of Potential Early Biomarkers for Gestational Diabetes Mellitus: Insights from PTRPG and IGKV2D-28 Expression Analysis. Int. J. Mol. Sci. 2024, 25, 10527. https://doi.org/10.3390/ijms251910527
Payot MD, Villavieja A, Pineda-Cortel MR. Preliminary Investigation of Potential Early Biomarkers for Gestational Diabetes Mellitus: Insights from PTRPG and IGKV2D-28 Expression Analysis. International Journal of Molecular Sciences. 2024; 25(19):10527. https://doi.org/10.3390/ijms251910527
Chicago/Turabian StylePayot, Mariejim Diane, Adrian Villavieja, and Maria Ruth Pineda-Cortel. 2024. "Preliminary Investigation of Potential Early Biomarkers for Gestational Diabetes Mellitus: Insights from PTRPG and IGKV2D-28 Expression Analysis" International Journal of Molecular Sciences 25, no. 19: 10527. https://doi.org/10.3390/ijms251910527