Genome-Wide Analysis Elucidates the Roles of AhLBD Genes in Different Abiotic Stresses and Growth and Development Stages in the Peanut (Arachis hypogea L.)
Abstract
:1. Introduction
2. Results
2.1. Identification of LBD Family Genes in Peanut
2.2. Phylogenetic Analysis of AhLBDs in Peanut and Arabidopsis
2.3. Chromosomal Distribution and Gene Duplication Analysis of AhLBDs
2.4. Gene Structure and Conserved Protein Motifs Analysis of AhLBDs
2.5. Conserved Sequence Alignment of AhLBDs
2.6. Analysis of Cis-Acting Elements in the Promoter Regions of AhLBDs
2.7. Evolutionary Analysis of LBDs between A. hypogaea and Other Species
2.8. GO Enrichment Analysis of AhLBDs
2.9. Interaction Network Analysis of AhLBDs in Peanut Plants
2.10. Expression Profiles of AhLBDs in Different Developmental Stages and Abiotic Stress
2.11. qRT-PCR Identification of 12 Selected AhLBDs in Different Tissues
2.12. qRT-PCR Analysis of 12 AhLBDs under Salt and Drought Stresses in Peanut Plants
2.13. Subcellular Localization Analysis of Four AhLBDs
2.14. Overexpression of AhLBD33 and AhLBD67 in Arabidopsis thaliana and Salt Stress Test
3. Discussion
3.1. Comparison of Phylogenetic Relationships between the Peanut Plant LBD Family Genes and Other Species
3.2. The Potential Biological Functions of AhLBDs in Peanut Plants
4. Materials and Methods
4.1. Identification and Physicochemical Properties of the AhLBD Gene Family
4.2. Phylogenetic Analysis of AhLBD Proteins
4.3. Analysis of Chromosome Location and Synteny Analysis of AhLBDs
4.4. Gene Structure, Conserved Motif, and Promoter Cis-Element Analysis
4.5. Protein–Protein Interaction Network and Gene Ontology (GO) Analysis of AhLBDs
4.6. RNA-Seq Based Expression Profiling of AhLBDs
4.7. Plant Material, Abiotic Stress Treatments and qRT-PCR Analysis
4.8. Subcellular Localization Assay
4.9. Transformation of Arabidopsis thaliana Overexpressing AhLBD33 and AhLBD67 and Salt Stress
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wu, C.; Hou, B.; Wu, R.; Yang, L.; Lan, G.; Xia, Z.; Cao, C.; Pan, Z.; Lv, B.; Li, P. Genome-Wide Analysis Elucidates the Roles of AhLBD Genes in Different Abiotic Stresses and Growth and Development Stages in the Peanut (Arachis hypogea L.). Int. J. Mol. Sci. 2024, 25, 10561. https://doi.org/10.3390/ijms251910561
Wu C, Hou B, Wu R, Yang L, Lan G, Xia Z, Cao C, Pan Z, Lv B, Li P. Genome-Wide Analysis Elucidates the Roles of AhLBD Genes in Different Abiotic Stresses and Growth and Development Stages in the Peanut (Arachis hypogea L.). International Journal of Molecular Sciences. 2024; 25(19):10561. https://doi.org/10.3390/ijms251910561
Chicago/Turabian StyleWu, Cuicui, Baoguo Hou, Rilian Wu, Liuliu Yang, Gang Lan, Zhi Xia, Cairong Cao, Zhuanxia Pan, Beibei Lv, and Pengbo Li. 2024. "Genome-Wide Analysis Elucidates the Roles of AhLBD Genes in Different Abiotic Stresses and Growth and Development Stages in the Peanut (Arachis hypogea L.)" International Journal of Molecular Sciences 25, no. 19: 10561. https://doi.org/10.3390/ijms251910561