Genome-Wide Identification and Characterization of Diterpenoid Pathway CYPs in Andrographis paniculata and Analysis of Their Expression Patterns under Low Temperature Stress
Abstract
:1. Introduction
2. Results
2.1. Identification and Classification of Total CYPs in Andrographis Paniculata
2.2. Identification of Diterpenoid Pathway CYPs in Andrographis Paniculata
2.3. Analysis of Basic Information for Diterpenoid Pathway CYPs
2.4. Analysis of Feature Information for Diterpenoid Pathway CYPs
2.5. Structural Analysis of Representative Diterpenoid Pathway CYPs
2.6. Protein Interactions of LT-Responsive Representative CYPs
2.7. LT Expression Profiling of KO, KAO, and CYP71D-Interacting Genes
3. Discussion
4. Materials and Methods
4.1. Identification of Total CYPs in Andrographis Paniculata
4.2. Construction of Phylogenetic Trees
4.3. Identification of Conserved Domains in Andrographis Paniculata CYPs
4.4. Analysis of Gene Structure
4.5. Analysis of Protein Characteristics
4.6. Quantification of Andrographolide Content
4.6.1. Growth Conditions
4.6.2. LT Treatment Conditions
4.6.3. Detection of Andrographolide Using Liquid Chromatography–Tandem Mass Spectrometry
4.6.4. Statistical Analysis
4.7. Gene Expression Analysis under LT Treatment
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | CYP Subfamily | Number of Andrographis Paniculata CYPs | Number of Arabidopsis Thaliana CYPs | Number of Terrestrial Plants Diterpenoid Pathway CYPs |
---|---|---|---|---|
1 | 51 | 1 | 1 | 0 |
2 | 71 | 80 | 48 | 11 |
3 | 72 | 20 | 8 | 0 |
4 | 73 | 2 | 1 | 0 |
5 | 74 | 3 | 2 | 0 |
6 | 75 | 7 | 1 | 0 |
7 | 76 | 23 | 9 | 26 |
8 | 77 | 3 | 4 | 0 |
9 | 78 | 8 | 6 | 0 |
10 | 79 | 8 | 6 | 0 |
11 | 81 | 8 | 16 | 0 |
12 | 82 | 19 | 5 | 0 |
13 | 83 | 15 | 3 | 0 |
14 | 84 | 4 | 1 | 0 |
15 | 85 | 3 | 1 | 0 |
16 | 86 | 17 | 10 | 0 |
17 | 87 | 5 | 1 | 0 |
18 | 88 | 2 | 2 | 12 |
19 | 89 | 0 | 6 | 0 |
20 | 90 | 9 | 4 | 0 |
21 | 93 | 4 | 1 | 0 |
22 | 94 | 12 | 6 | 0 |
23 | 96 | 0 | 12 | 0 |
24 | 97 | 4 | 3 | 0 |
25 | 98 | 2 | 3 | 0 |
26 | 701 | 1 | 1 | 23 |
27 | 702 | 0 | 6 | 0 |
28 | 703 | 1 | 1 | 0 |
29 | 704 | 5 | 3 | 0 |
30 | 705 | 0 | 25 | 0 |
31 | 706 | 2 | 7 | 0 |
32 | 707 | 10 | 4 | 0 |
33 | 708 | 0 | 3 | 0 |
34 | 709 | 2 | 3 | 0 |
35 | 710 | 1 | 4 | 0 |
36 | 711 | 1 | 1 | 0 |
37 | 712 | 0 | 2 | 0 |
38 | 714 | 4 | 2 | 5 |
39 | 715 | 5 | 1 | 0 |
40 | 716 | 13 | 2 | 0 |
41 | 718 | 1 | 1 | 0 |
42 | 720 | 1 | 1 | 0 |
43 | 721 | 0 | 1 | 0 |
44 | 722 | 2 | 1 | 0 |
45 | 724 | 2 | 1 | 0 |
46 | 726 | 0 | 0 | 14 |
47 | 734 | 4 | 1 | 0 |
48 | 735 | 1 | 2 | 0 |
49 | 736 | 8 | 0 | 0 |
50 | 749 | 5 | 0 | 0 |
51 | CYP family | 17 | 0 | 0 |
Total | 345 | 233 | 91 |
Subfamily | Gene Number | Number of Cis-Elements | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ABA | Anaerobic | Auxin | Stress | GAs | LT | MeJA | Drought | Circadian | Salicylic Acid | Cell Differentiation | Flavonoid Biosynthesis | ||
CYP88A | 2 | 1 | 6 | 4 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
CYP701A | 1 | 1 | 3 | 0 | 0 | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
CYP714s | 4 | 5 | 6 | 2 | 3 | 5 | 8 | 4 | 2 | 2 | 4 | 0 | 0 |
CYP76s | 3 | 17 | 7 | 3 | 2 | 3 | 6 | 5 | 7 | 3 | 2 | 1 | 0 |
CYP71s | 55 | 120 | 78 | 30 | 25 | 40 | 53 | 97 | 29 | 25 | 55 | 4 | 2 |
Total | 65 | 144 | 100 | 39 | 31 | 53 | 68 | 109 | 39 | 30 | 61 | 5 | 2 |
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Sun, M.; Li, J.; Xu, S.; Gu, Y.; Wang, J. Genome-Wide Identification and Characterization of Diterpenoid Pathway CYPs in Andrographis paniculata and Analysis of Their Expression Patterns under Low Temperature Stress. Int. J. Mol. Sci. 2024, 25, 10741. https://doi.org/10.3390/ijms251910741
Sun M, Li J, Xu S, Gu Y, Wang J. Genome-Wide Identification and Characterization of Diterpenoid Pathway CYPs in Andrographis paniculata and Analysis of Their Expression Patterns under Low Temperature Stress. International Journal of Molecular Sciences. 2024; 25(19):10741. https://doi.org/10.3390/ijms251910741
Chicago/Turabian StyleSun, Mingyang, Jingyu Li, Shiqiang Xu, Yan Gu, and Jihua Wang. 2024. "Genome-Wide Identification and Characterization of Diterpenoid Pathway CYPs in Andrographis paniculata and Analysis of Their Expression Patterns under Low Temperature Stress" International Journal of Molecular Sciences 25, no. 19: 10741. https://doi.org/10.3390/ijms251910741