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Volume 25
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Communication

Knockout of the Tnfa Gene Decreases Influenza Virus-Induced Histological Reactions in Laboratory Mice

by
Darya A. Savenkova
1,2,†,
Andrey S. Gudymo
1,†,
Alexey N. Korablev
1,3,
Oleg S. Taranov
1,
Darya V. Bazovkina
3,
Nataliya V. Danilchenko
1,
Olga N. Perfilyeva
1,
Elena K. Ivleva
1,
Anastasiya A. Moiseeva
1,
Yulia A. Bulanovich
1,
Elena V. Roshchina
1,
Irina A. Serova
3,
Nariman R. Battulin
2,3,
Elizabeth A. Kulikova
3 and
Dmitry V. Yudkin
1,*
1
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo 630559, Russia
2
Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia
3
Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Lavrentieva 10, Novosibirsk 630090, Russia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(2), 1156; https://doi.org/10.3390/ijms25021156
Submission received: 12 December 2023 / Revised: 12 January 2024 / Accepted: 15 January 2024 / Published: 18 January 2024
(This article belongs to the Special Issue Influenza Viruses: Infection and Genomics)
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Abstract

Tumor necrosis factor alpha (TNF-α) is a cytokine that is responsible for many processes associated with immune response and inflammation. It is involved in the development of an antiviral response to many virus infections. This factor was shown to be activated in influenza A virus infection, which enhances production of other cytokines. The overexpression of these cytokines can lead to a cytokine storm. To study the role of TNF-α in the development of pathologies associated with viral infection, we generated a Tnfa knockout mouse strain. We demonstrated that these mice were characterized by a significant increase in the number of viral genomes compared to that in the parental strain, but the amount of live virus did not differ. A histopathology of the lungs in the genetically modified animals was significantly lower in terms of interalveolar septal infiltration. The generated model may be used to further study pathological processes in viral infections.
Keywords: TNF-α; influenza A virus; H1N1; knockout mice; CRISPR/Cas9 TNF-α; influenza A virus; H1N1; knockout mice; CRISPR/Cas9

Share and Cite

MDPI and ACS Style

Savenkova, D.A.; Gudymo, A.S.; Korablev, A.N.; Taranov, O.S.; Bazovkina, D.V.; Danilchenko, N.V.; Perfilyeva, O.N.; Ivleva, E.K.; Moiseeva, A.A.; Bulanovich, Y.A.; et al. Knockout of the Tnfa Gene Decreases Influenza Virus-Induced Histological Reactions in Laboratory Mice. Int. J. Mol. Sci. 2024, 25, 1156. https://doi.org/10.3390/ijms25021156

AMA Style

Savenkova DA, Gudymo AS, Korablev AN, Taranov OS, Bazovkina DV, Danilchenko NV, Perfilyeva ON, Ivleva EK, Moiseeva AA, Bulanovich YA, et al. Knockout of the Tnfa Gene Decreases Influenza Virus-Induced Histological Reactions in Laboratory Mice. International Journal of Molecular Sciences. 2024; 25(2):1156. https://doi.org/10.3390/ijms25021156

Chicago/Turabian Style

Savenkova, Darya A., Andrey S. Gudymo, Alexey N. Korablev, Oleg S. Taranov, Darya V. Bazovkina, Nataliya V. Danilchenko, Olga N. Perfilyeva, Elena K. Ivleva, Anastasiya A. Moiseeva, Yulia A. Bulanovich, and et al. 2024. "Knockout of the Tnfa Gene Decreases Influenza Virus-Induced Histological Reactions in Laboratory Mice" International Journal of Molecular Sciences 25, no. 2: 1156. https://doi.org/10.3390/ijms25021156

APA Style

Savenkova, D. A., Gudymo, A. S., Korablev, A. N., Taranov, O. S., Bazovkina, D. V., Danilchenko, N. V., Perfilyeva, O. N., Ivleva, E. K., Moiseeva, A. A., Bulanovich, Y. A., Roshchina, E. V., Serova, I. A., Battulin, N. R., Kulikova, E. A., & Yudkin, D. V. (2024). Knockout of the Tnfa Gene Decreases Influenza Virus-Induced Histological Reactions in Laboratory Mice. International Journal of Molecular Sciences, 25(2), 1156. https://doi.org/10.3390/ijms25021156

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