APRF1 Interactome Reveals HSP90 as a New Player in the Complex That Epigenetically Regulates Flowering Time in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. APRF1-Impaired Plants Display a Temperature-Dependent Flowering Time Phenotype
2.2. APRF1 Protein Is Localized Both in the Nucleus and the Cytoplasm
2.3. APRF1 Co-IP Resulted in 471 Non-Redundant Putative Interacting Proteins
2.4. Functional Enrichment Profiling
2.5. Protein–Protein Interaction Analysis
2.6. Molecular Docking Analysis of APRF1 with HSP90.1 and HSP90.2
2.7. APRF1 and HSP90 Chaperons Functionally Interact in Planta
3. Discussion
4. Materials and Methods
4.1. Plants Growth and Treatments
4.2. PCR, Generation of Binary Vectors, BiFC, and Plant Transformation
4.3. Protein Extraction
4.4. Protein Co-Immunoprecipitation
4.5. Western Blotting
4.6. Mass Spectrometry
4.7. Microscopy
4.8. Functional Enrichment Analysis and Assessment of Protein–Protein Interactions via STRING
4.9. Alphafold2 Structures and Molecular Docking
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isaioglou, I.; Podia, V.; Velentzas, A.D.; Kapolas, G.; Beris, D.; Karampelias, M.; Plitsi, P.K.; Chatzopoulos, D.; Samakovli, D.; Roussis, A.; et al. APRF1 Interactome Reveals HSP90 as a New Player in the Complex That Epigenetically Regulates Flowering Time in Arabidopsis thaliana. Int. J. Mol. Sci. 2024, 25, 1313. https://doi.org/10.3390/ijms25021313
Isaioglou I, Podia V, Velentzas AD, Kapolas G, Beris D, Karampelias M, Plitsi PK, Chatzopoulos D, Samakovli D, Roussis A, et al. APRF1 Interactome Reveals HSP90 as a New Player in the Complex That Epigenetically Regulates Flowering Time in Arabidopsis thaliana. International Journal of Molecular Sciences. 2024; 25(2):1313. https://doi.org/10.3390/ijms25021313
Chicago/Turabian StyleIsaioglou, Ioannis, Varvara Podia, Athanassios D. Velentzas, Georgios Kapolas, Despoina Beris, Michael Karampelias, Panagiota Konstantinia Plitsi, Dimitris Chatzopoulos, Despina Samakovli, Andreas Roussis, and et al. 2024. "APRF1 Interactome Reveals HSP90 as a New Player in the Complex That Epigenetically Regulates Flowering Time in Arabidopsis thaliana" International Journal of Molecular Sciences 25, no. 2: 1313. https://doi.org/10.3390/ijms25021313