First Evidence of Mineralocorticoid Receptor Gene and Protein Expression in Rat and Human Thyroid Tissues and Cell Cultures
Abstract
:1. Introduction
2. Results
2.1. Gene Expression of Mineralocorticoid Receptor in Thyroid Tissues and Cell Lines
2.2. Mineralocorticoid Receptor Protein Expression, Localisation, and Activation in FRTL-5
2.3. Effects of Aldosterone on Target Genes Involved in Inflammation in the FRTL-5 Cell Line
2.4. Effects of Aldosterone on Thyroid-Specific Genes in the FRTL-5 Cell Line
2.5. Mineralocorticoid Receptor Protein Expression, Localisation, and Activation in PTC Cell Lines
2.6. Effects of Aldosterone in PTC Cell Lines
2.7. Mineralocorticoid Receptor Gene and Protein Expression in Human PTC
2.8. Mineralocorticoid Receptor Gene Expression in Aggressive Thyroid Cancers
3. Discussion
4. Materials and Method
4.1. Rat and Human Tissues
4.2. Cell Cultures and Maintenance
4.3. Drugs
4.4. RNA Extraction, cDNA Synthesis, and qRT-PCR Analysis
4.5. Immunofluorescence and Confocal Microscopy
4.6. Western Blot Analysis of Human Tissues
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Assay ID | Gene | Gene Symbol | Cat. No. |
---|---|---|---|
Hs01031809_m1 | Human Mineralocorticoid Receptor (MR) | NR3C2 | 4331182 |
Hs001741341_m1 | Human Interluekin 6 | IL-6 | 4331182 |
Hs00998133_m1 | Human Transforming Growth Factor-β | TGFβ | 4331182 |
Hs00892519_m1 | Human Thyroid Peroxidase | TPO | 4331182 |
Hs00174974_m1 | Human Thyreoglobulin | TG | 4331182 |
Hs00166567_m1 | Human Sodium/Iodide symporter (NIS) | SLC5A5 | 4331182 |
Hs00388669_m1 | Human Hydroxysteroid 11-β dehydrogenase 2 | HSD11B2 | 4331182 |
Rn00565562_m1 | Rat Mineralocorticoid Receptor (MR) | NR3C2 | 4448892 |
Rn01410330_m1 | Rat Interluekin 6 | IL-6 | 4448892 |
Rn00572010_m1 | Rat Transforming Growth Factor-β | TGFβ | 4448892 |
Rn00571159_m1 | Rat Thyroid Peroxidase | TPO | 4448892 |
Rn00667257_g1 | Rat Thyreoglobulin | TG | 4448892 |
Rn00583900_m1 | Rat Sodium/Iodide Symporter (NIS) | SLC5A5 | 4448892 |
Rn00492539_m1 | Rat Hydroxysteroid 11-β Dehydrogenase 2 | HSD11B2 | 4448892 |
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Manso, J.; Pedron, M.C.; Mondin, A.; Censi, S.; Pennelli, G.; Galuppini, F.; Barollo, S.; Bertazza, L.; Radu, C.M.; Ghini, F.; et al. First Evidence of Mineralocorticoid Receptor Gene and Protein Expression in Rat and Human Thyroid Tissues and Cell Cultures. Int. J. Mol. Sci. 2024, 25, 754. https://doi.org/10.3390/ijms25020754
Manso J, Pedron MC, Mondin A, Censi S, Pennelli G, Galuppini F, Barollo S, Bertazza L, Radu CM, Ghini F, et al. First Evidence of Mineralocorticoid Receptor Gene and Protein Expression in Rat and Human Thyroid Tissues and Cell Cultures. International Journal of Molecular Sciences. 2024; 25(2):754. https://doi.org/10.3390/ijms25020754
Chicago/Turabian StyleManso, Jacopo, Maria Chiara Pedron, Alberto Mondin, Simona Censi, Gianmaria Pennelli, Francesca Galuppini, Susi Barollo, Loris Bertazza, Claudia Maria Radu, Francesca Ghini, and et al. 2024. "First Evidence of Mineralocorticoid Receptor Gene and Protein Expression in Rat and Human Thyroid Tissues and Cell Cultures" International Journal of Molecular Sciences 25, no. 2: 754. https://doi.org/10.3390/ijms25020754