Identification of Potato StPIN Gene Family and Regulation of Root Development by StPIN4
Abstract
:1. Introduction
2. Results
2.1. Identification and Analysis of Potato StPIN Family Genes
2.2. Chromosomal Localization and Gene Structure Analysis of Potato PIN Gene Family Members
2.3. Potato StPINs Protein Family Motif and Phylogenetic Analysis
2.4. Analysis of Cis-Acting Elements of Potato PIN Gene Family Members
2.5. Expression of StPIN Gamily Genes During Potato Root Development
2.6. Tissue Differential Expression Analysis of StPIN4 Gene
2.7. Acquisition and Assay of Transgenic Plants
2.8. qRT-PCR Analysis and RNA Validation
2.9. Phenotypic Assay of Transgenic Potato
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification and Annotation of Potato PIN Gene Family Members
4.3. Bioinformatics Analysis of StPINs
4.4. Gene Expression Analysis of StPINs
4.5. Construction of Expression Vectors for Potato Transgenic Plants
4.6. RNA Extraction and qRT-PCR
4.7. Analysis of StPIN4 Regulates Potato Root Development
4.8. Data Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name | Gene ID | Number of AA | Molecular Weight | pI | Instability Coefficient | Hydrophilicity Index | Subcellular Localization |
---|---|---|---|---|---|---|---|
StPIN1 | Soltu.DM.01G025960.1 | 235 | 25,398.31 | 9.22 | 42.42 | 0.793 | Plasma Membrane |
StPIN2 | Soltu.DM.01G025980.1 | 355 | 39,264.82 | 9.13 | 36.26 | 0.762 | Plasma Membrane |
StPIN3 | Soltu.DM.02G032050.1 | 358 | 39,084.99 | 9.18 | 34.90 | 0.793 | Plasma Membrane |
StPIN4 | Soltu.DM.03G033110.1 | 614 | 67,134.08 | 9.08 | 37.51 | 0.030 | Plasma Membrane |
StPIN5 | Soltu.DM.04G002690.1 | 622 | 68,279.87 | 6.57 | 39.89 | 0.141 | Plasma Membrane |
StPIN6 | Soltu.DM.04G020760.1 | 349 | 38,620.75 | 6.58 | 35.82 | 0.681 | Plasma Membrane |
StPIN7 | Soltu.DM.05G004900.1 | 745 | 81,967.28 | 7.05 | 41.95 | 0.085 | Plasma Membrane |
StPIN8 | Soltu.DM.06G016050.1 | 531 | 57,957.83 | 8.85 | 33.17 | 0.408 | Plasma Membrane |
StPIN9 | Soltu.DM.07G002230.1 | 631 | 68,666.48 | 9.14 | 42.51 | 0.166 | Plasma Membrane |
StPIN10 | Soltu.DM.10G026500.1 | 587 | 63,906.07 | 8.81 | 34.67 | 0.233 | Plasma Membrane |
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Zhang, Q.; Liu, Q.; Yang, J.; Zhang, N.; Si, H. Identification of Potato StPIN Gene Family and Regulation of Root Development by StPIN4. Int. J. Mol. Sci. 2024, 25, 11517. https://doi.org/10.3390/ijms252111517
Zhang Q, Liu Q, Yang J, Zhang N, Si H. Identification of Potato StPIN Gene Family and Regulation of Root Development by StPIN4. International Journal of Molecular Sciences. 2024; 25(21):11517. https://doi.org/10.3390/ijms252111517
Chicago/Turabian StyleZhang, Qian, Qing Liu, Jiangwei Yang, Ning Zhang, and Huaijun Si. 2024. "Identification of Potato StPIN Gene Family and Regulation of Root Development by StPIN4" International Journal of Molecular Sciences 25, no. 21: 11517. https://doi.org/10.3390/ijms252111517