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Article

Trehalose Alleviates D-Galactose-Induced Aging-Related Granulosa Cell Death in Ovaries

by
Huaming Xi
†,
Xinyu Chen
†,
Kai Liang
,
Xianglong Wang
,
Feng Jiang
,
Yuan Li
* and
Dong Niu
*
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(23), 12643; https://doi.org/10.3390/ijms252312643
Submission received: 1 November 2024 / Revised: 19 November 2024 / Accepted: 23 November 2024 / Published: 25 November 2024
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

Ovarian dysfunction caused by aging restricts female reproductive capacity and is accompanied by oxidative stress and impaired autophagy. Recent studies have shown that trehalose (Tre) can activate autophagy and have antioxidant effects. However, whether Tre can be used to attenuate ovarian aging remains unclear. Therefore, the anti-aging effects of Tre on the ovary were explored both in vivo and in vitro. D-galactose (D-gal) was administered i.p. daily (200 mg/kg body weight) for 8 weeks to establish the mouse ovarian aging model (n = 10). We found that Tre significantly reversed ovarian weight loss and reduced the number of TUNEL-positive granulosa cells caused by D-gal in mouse ovaries. Tre elevated the protein expression levels of LC3-II, Parkin, PINK1, Beclin1, and LAMP2 in ovaries. Mitochondrial-related proteins TOM20 and COX IV expression levels were increased by Tre administration. In vitro studies further supported these findings, showing that Tre treatment significantly reduced the number of SA-β-gal and PI-positive cells, and decreased ROS levels in cultured granulosa cells. Thus, Tre alleviates ovarian aging by activating mitophagy and reducing oxidative stress, suggesting its potential as an anti-aging agent for ovarian health.
Keywords: trehalose; granulosa cell; aging; mitophagy; ovary trehalose; granulosa cell; aging; mitophagy; ovary

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MDPI and ACS Style

Xi, H.; Chen, X.; Liang, K.; Wang, X.; Jiang, F.; Li, Y.; Niu, D. Trehalose Alleviates D-Galactose-Induced Aging-Related Granulosa Cell Death in Ovaries. Int. J. Mol. Sci. 2024, 25, 12643. https://doi.org/10.3390/ijms252312643

AMA Style

Xi H, Chen X, Liang K, Wang X, Jiang F, Li Y, Niu D. Trehalose Alleviates D-Galactose-Induced Aging-Related Granulosa Cell Death in Ovaries. International Journal of Molecular Sciences. 2024; 25(23):12643. https://doi.org/10.3390/ijms252312643

Chicago/Turabian Style

Xi, Huaming, Xinyu Chen, Kai Liang, Xianglong Wang, Feng Jiang, Yuan Li, and Dong Niu. 2024. "Trehalose Alleviates D-Galactose-Induced Aging-Related Granulosa Cell Death in Ovaries" International Journal of Molecular Sciences 25, no. 23: 12643. https://doi.org/10.3390/ijms252312643

APA Style

Xi, H., Chen, X., Liang, K., Wang, X., Jiang, F., Li, Y., & Niu, D. (2024). Trehalose Alleviates D-Galactose-Induced Aging-Related Granulosa Cell Death in Ovaries. International Journal of Molecular Sciences, 25(23), 12643. https://doi.org/10.3390/ijms252312643

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