Integration and Differentiation of Transplanted Human iPSC-Derived Retinal Ganglion Cell Precursors in Murine Retinas
Abstract
:1. Introduction
2. Results
2.1. Differentiation of 3D Retinal Organoids from Wild-Type (WT) hiPSCs and BRN3B-GFP hiPSCs
2.2. Enrichment of Human RGC Precursors from Retinal Organoids for Transplantation
2.3. Survival, Integration, and Differentiation of Transplanted Human RGC Precursors in Mouse Retinas
2.4. Enhancement of the Survival and Integration of Transplanted RGC Precursors with Immunosuppressants
3. Discussion
4. Materials and Methods
4.1. Maintenance of hiPSCs
4.2. Generation of BRN3B-GFP hiPSC Line
4.3. Karyotyping of hiPSCs
4.4. Induction of 3D Retinal Organoids from hiPSCs
4.5. Dissociation of Retinal Organoid Cells and Enrichment of RGC Precursors
4.6. Transplantation of RGC Precursors
4.7. Immunohistochemistry
4.8. Quantification and Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lei, Q.; Zhang, R.; Yuan, F.; Xiang, M. Integration and Differentiation of Transplanted Human iPSC-Derived Retinal Ganglion Cell Precursors in Murine Retinas. Int. J. Mol. Sci. 2024, 25, 12947. https://doi.org/10.3390/ijms252312947
Lei Q, Zhang R, Yuan F, Xiang M. Integration and Differentiation of Transplanted Human iPSC-Derived Retinal Ganglion Cell Precursors in Murine Retinas. International Journal of Molecular Sciences. 2024; 25(23):12947. https://doi.org/10.3390/ijms252312947
Chicago/Turabian StyleLei, Qiannan, Rong Zhang, Fa Yuan, and Mengqing Xiang. 2024. "Integration and Differentiation of Transplanted Human iPSC-Derived Retinal Ganglion Cell Precursors in Murine Retinas" International Journal of Molecular Sciences 25, no. 23: 12947. https://doi.org/10.3390/ijms252312947
APA StyleLei, Q., Zhang, R., Yuan, F., & Xiang, M. (2024). Integration and Differentiation of Transplanted Human iPSC-Derived Retinal Ganglion Cell Precursors in Murine Retinas. International Journal of Molecular Sciences, 25(23), 12947. https://doi.org/10.3390/ijms252312947