Chronic Morphine Treatment and Antiretroviral Therapy Exacerbate HIV-Distal Sensory Peripheral Neuropathy and Induce Distinct Microbial Alterations in the HIV Tg26 Mouse Model

Antoine, Danielle; Chupikova, Irina; Jalodia, Richa; Singh, Praveen Kumar; Roy, Sabita

doi:10.3390/ijms25031569

Open AccessArticle

Chronic Morphine Treatment and Antiretroviral Therapy Exacerbate HIV-Distal Sensory Peripheral Neuropathy and Induce Distinct Microbial Alterations in the HIV Tg26 Mouse Model

by

Danielle Antoine

^1,2,†

,

Irina Chupikova

^1,†,

Richa Jalodia

¹,

Praveen Kumar Singh

¹ and

Sabita Roy

^1,*

¹

Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL 33136, USA

²

Department of Neuroscience, Miller School of Medicine, University of Miami, Miami, FL 33136, USA

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2024, 25(3), 1569; https://doi.org/10.3390/ijms25031569

Submission received: 5 December 2023 / Revised: 8 January 2024 / Accepted: 10 January 2024 / Published: 26 January 2024

(This article belongs to the Special Issue New Insights into the Use of Mouse Models for the Study of Skin Disorders)

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Abstract

Distal Sensory Peripheral Neuropathy (DSP) is a common complication in HIV-infected individuals, leading to chronic pain and reduced quality of life. Even with antiretroviral therapy (ART), DSP persists, often prompting the use of opioid analgesics, which can paradoxically worsen symptoms through opioid-induced microbial dysbiosis. This study employs the HIV Tg26 mouse model to investigate HIV-DSP development and assess gut microbiome changes in response to chronic morphine treatment and ART using 16S rRNA sequencing. Our results reveal that chronic morphine and ART exacerbate HIV-DSP in Tg26 mice, primarily through mechanical pain pathways. As the gut microbiome may be involved in chronic pain persistence, microbiome analysis indicated distinct bacterial community changes between WT and Tg26 mice as well as morphine- and ART-induced microbial changes in the Tg26 mice. This study reveals the Tg26 mouse model to be a relevant system that can help elucidate the pathogenic mechanisms of the opioid- and ART-induced exacerbation of HIV-associated pain. Our results shed light on the intricate interplay between HIV infection, ART, opioid use, and the gut microbiome in chronic pain development. They hold implications for understanding the mechanisms underlying HIV-associated pain and microbial dysbiosis, with potential for future research focused on prevention and treatment strategies.

Keywords: HIV-associated pain; neuropathy; opioid use; antiretroviral therapy; gut dysbiosis; Tg26 mouse model

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MDPI and ACS Style

Antoine, D.; Chupikova, I.; Jalodia, R.; Singh, P.K.; Roy, S. Chronic Morphine Treatment and Antiretroviral Therapy Exacerbate HIV-Distal Sensory Peripheral Neuropathy and Induce Distinct Microbial Alterations in the HIV Tg26 Mouse Model. Int. J. Mol. Sci. 2024, 25, 1569. https://doi.org/10.3390/ijms25031569

AMA Style

Antoine D, Chupikova I, Jalodia R, Singh PK, Roy S. Chronic Morphine Treatment and Antiretroviral Therapy Exacerbate HIV-Distal Sensory Peripheral Neuropathy and Induce Distinct Microbial Alterations in the HIV Tg26 Mouse Model. International Journal of Molecular Sciences. 2024; 25(3):1569. https://doi.org/10.3390/ijms25031569

Chicago/Turabian Style

Antoine, Danielle, Irina Chupikova, Richa Jalodia, Praveen Kumar Singh, and Sabita Roy. 2024. "Chronic Morphine Treatment and Antiretroviral Therapy Exacerbate HIV-Distal Sensory Peripheral Neuropathy and Induce Distinct Microbial Alterations in the HIV Tg26 Mouse Model" International Journal of Molecular Sciences 25, no. 3: 1569. https://doi.org/10.3390/ijms25031569

APA Style

Antoine, D., Chupikova, I., Jalodia, R., Singh, P. K., & Roy, S. (2024). Chronic Morphine Treatment and Antiretroviral Therapy Exacerbate HIV-Distal Sensory Peripheral Neuropathy and Induce Distinct Microbial Alterations in the HIV Tg26 Mouse Model. International Journal of Molecular Sciences, 25(3), 1569. https://doi.org/10.3390/ijms25031569

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Chronic Morphine Treatment and Antiretroviral Therapy Exacerbate HIV-Distal Sensory Peripheral Neuropathy and Induce Distinct Microbial Alterations in the HIV Tg26 Mouse Model

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