Niemann–Pick Type C2 Proteins in Aedes aegypti: Molecular Modelling and Prediction of Their Structure–Function Relationships
Abstract
:1. Introduction
2. Results
2.1. Sequence Analysis
2.2. Genome-Wide Distribution
2.3. Intron–Exon Structures of NPC2 Genes
2.4. Structure–Function Relationships of NPC2 Proteins
2.4.1. NPC2 Proteins in Cholesterol Binding and Transport
2.4.2. Molecular Modelling of Potential Cholesterol-Binding NPC2 Proteins
2.4.3. NPC2 Proteins in Semiochemical Binding and Communication
2.4.4. Molecular Modelling of Potential Fatty-Acid-Binding NPC2 Protein
2.5. Expression of Potential Cholesterol- and Fatty-Acid-Binding NPC2 Genes
2.6. Phylogenetic Analysis
2.7. Expression of NPC2 Genes Post Arbovirus Infection
2.7.1. Differential Expression in the Midgut
2.7.2. Differential Expression in the Salivary Gland
3. Discussion
4. Materials and Methods
4.1. Analyses of Nucleotide and Protein Sequences of NPC2 Genes
4.2. Structure–Function Relationships of NPC2 Proteins
4.3. Molecular Modelling of NPC2 Proteins
4.4. Comparison and Superimposition of NPC2 Proteins
4.5. Expression of NPC2 Genes in Mosquito Tissues
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Thambi, P.J.; Modahl, C.M.; Kini, R.M. Niemann–Pick Type C2 Proteins in Aedes aegypti: Molecular Modelling and Prediction of Their Structure–Function Relationships. Int. J. Mol. Sci. 2024, 25, 1684. https://doi.org/10.3390/ijms25031684
Thambi PJ, Modahl CM, Kini RM. Niemann–Pick Type C2 Proteins in Aedes aegypti: Molecular Modelling and Prediction of Their Structure–Function Relationships. International Journal of Molecular Sciences. 2024; 25(3):1684. https://doi.org/10.3390/ijms25031684
Chicago/Turabian StyleThambi, Prathigna Jaishankar, Cassandra M. Modahl, and R. Manjunatha Kini. 2024. "Niemann–Pick Type C2 Proteins in Aedes aegypti: Molecular Modelling and Prediction of Their Structure–Function Relationships" International Journal of Molecular Sciences 25, no. 3: 1684. https://doi.org/10.3390/ijms25031684