Chemogenetics Modulation of Electroacupuncture Analgesia in Mice Spared Nerve Injury-Induced Neuropathic Pain through TRPV1 Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Electroacupuncture at Mice ST36 Acupoint Ameliorated Mechanical and Thermal Hyperalgesia in a Spared Nerve Injury-Initiated Chronic Pain Model
2.2. Electroacupuncture at ST36 Restored Elevated SNI-Derived Serum Inflammatory Mediators
2.3. Electroacupuncture Alleviated the Overexpression of Microglial Transmission and TRPV1-Related Kinases in Mice DRG
2.4. Electroacupuncture Alleviated Spared Nerve Injury-Induced Microglial and TRPV1 Increased Appearance in the Spinal Cord Dorsal Horn
2.5. Electroacupuncture Improved Microglial Hyperfunction and TRPV1 Activation in the Somatosensory Cortex after SNI Induction
2.6. Electroacupuncture Reversed Microglial Activity, TRPV1, and Associated Molecules Changes in the ACC after SNI
2.7. Chemogenetic Inhibition of SSC Improved Neuropathic Pain in the Mouse SNI Model
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Neuropathic Pain Model
4.3. Electroacupuncture
4.4. Nociceptive Behavioral Tests
4.5. Western Blot Analysis
4.6. Immunofluorescence
4.7. Chemogenetic Operation
4.8. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
- Rajaa, S.N.; Carrb, D.B.; Cohenc, M.; Finnerupd, N.B.; Florf, H.; Gibsong, S.; Keefeh, F.J.; Mogili, J.S.; Ringkampj, M.; Slukak, K.A.; et al. The revised International Association for the Study of Pain definition of pain: Concepts, challenges, and compromises. Pain 2020, 161, 1976–1982. [Google Scholar] [CrossRef]
- Baron, R.; Binder, A.; Wasner, G. Neuropathic pain: Diagnosis, pathophysiological mechanisms, and treatment. Lancet Neurol. 2010, 9, 807–819. [Google Scholar] [CrossRef]
- Hatch, M.N.; Cushing, T.R.; Carlson, G.D.; Chang, E.Y. Neuropathic pain and SCI: Identification and treatment strategies in the 21st century. J. Neurol. Sci. 2018, 384, 75–83. [Google Scholar] [CrossRef]
- Hsiao, I.H.; Lin, Y.W. Electroacupuncture Reduces Fibromyalgia Pain by Attenuating the HMGB1, S100B, and TRPV1 Signalling Pathways in the Mouse Brain. Evid. Based Complement. Alternat Med. 2022, 2022, 2242074. [Google Scholar] [CrossRef]
- Malmberg, A.B.; Basbaum, A.I. Partial sciatic nerve injury in the mouse as a model of neuropathic pain: Behavioral and neuroanatomical correlates. Pain 1998, 76, 215–222. [Google Scholar] [CrossRef] [PubMed]
- Sarzi-Puttini, P.; Giorgi, V.; Marotto, D.; Atzeni, F. Fibromyalgia: An update on clinical characteristics, aetiopathogenesis and treatment. Nat. Rev. Rheumatol. 2020, 16, 645–660. [Google Scholar] [CrossRef]
- Sommer, C.; Leinders, M.; Uceyler, N. Inflammation in the pathophysiology of neuropathic pain. Pain 2018, 159, 595–602. [Google Scholar] [CrossRef] [PubMed]
- Yam, M.F.; Loh, Y.C.; Tan, C.S.; Khadijah Adam, S.; Abdul Manan, N.; Basir, R. General Pathways of Pain Sensation and the Major Neurotransmitters Involved in Pain Regulation. Int. J. Mol. Sci. 2018, 19, 2164. [Google Scholar] [CrossRef]
- Yang, S.; Chang, M.C. Chronic Pain: Structural and Functional Changes in Brain Structures and Associated Negative Affective States. Int. J. Mol. Sci. 2019, 20, 3130. [Google Scholar] [CrossRef] [PubMed]
- Asgharzade, S.; Talaei, A.; Farkhondeh, T.; Forouzanfar, F. A Review on Stem Cell Therapy for Neuropathic Pain. Curr. Stem Cell Res. Ther. 2020, 15, 349–361. [Google Scholar] [CrossRef]
- Forouzanfar, F.; Tanha, N.K.; Pourbagher-Shahri, A.M.; Mahdianpour, S.; Esmaeili, M.; Ghazavi, H. Synergistic effect of ellagic acid and gabapentin in a rat model of neuropathic pain. Metab. Brain Dis. 2023, 38, 1421–1432. [Google Scholar] [CrossRef] [PubMed]
- Rakhshandeh, H.; Ghorbanzadeh, A.; Negah, S.S.; Akaberi, M.; Rashidi, R.; Forouzanfar, F. Pain-relieving effects of Lawsonia inermis on neuropathic pain induced by chronic constriction injury. Metab. Brain Dis. 2021, 36, 1709–1716. [Google Scholar] [CrossRef] [PubMed]
- Yang, C.; Yamaki, S.; Jung, T.; Kim, B.; Huyhn, R.; McKemy, D.D. Endogenous inflammatory mediators produced by injury activate TRPV1 and TRPA1 nociceptors to induce sexually dimorphic cold pain that is dependent on TRPM8 and GFRalpha3. bioRxiv 2023. [Google Scholar] [CrossRef]
- Caterina, M.J.; Schumacher, M.A.; Tominaga, M.; Rosen, T.A.; Levine, J.D.; Julius, D. The capsaicin receptor: A heat-activated ion channel in the pain pathway. Nature 1997, 389, 816–824. [Google Scholar] [CrossRef]
- Cichon, J.; Sun, L.; Yang, G. Spared Nerve Injury Model of Neuropathic Pain in Mice. Bio-protocol 2018, 8, e2777. [Google Scholar] [CrossRef]
- Lotze, M.T.; Tracey, K.J. High-mobility group box 1 protein (HMGB1): Nuclear weapon in the immune arsenal. Nat. Rev. Immunol. 2005, 5, 331–342. [Google Scholar] [CrossRef] [PubMed]
- Leclerc, E.; Fritz, G.; Vetter, S.W.; Heizmann, C.W. Binding of S100 proteins to RAGE: An update. Biochim. Biophys. Acta 2009, 1793, 993–1007. [Google Scholar] [CrossRef]
- Yan, B.; Tang, S.; Zhang, Y.; Xiao, X. The Role of Glia Underlying Acupuncture Analgesia in Animal Pain Models: A Systematic Review and Meta-Analysis. Pain. Med. 2023, 24, 11–24. [Google Scholar] [CrossRef]
- Han, J.S. Acupuncture: Neuropeptide release produced by electrical stimulation of different frequencies. Trends Neurosci. 2003, 26, 17–22. [Google Scholar] [CrossRef]
- Goldman, N.; Chen, M.; Fujita, T.; Xu, Q.; Peng, W.; Liu, W.; Jensen, T.K.; Pei, Y.; Wang, F.; Han, X.; et al. Adenosine A1 receptors mediate local anti-nociceptive effects of acupuncture. Nat. Neurosci. 2010, 13, 883–888. [Google Scholar] [CrossRef]
- Herrero, J.F.; Laird, J.M.; Lopez-Garcia, J.A. Wind-up of spinal cord neurones and pain sensation: Much ado about something? Prog. Neurobiol. 2000, 61, 169–203. [Google Scholar] [CrossRef]
- Zanjani, T.M.; Sabetkasaei, M.; Mosaffa, N.; Manaheji, H.; Labibi, F.; Farokhi, B. Suppression of interleukin-6 by minocycline in a rat model of neuropathic pain. Eur. J. Pharmacol. 2006, 538, 66–72. [Google Scholar] [CrossRef]
- Kiguchi, N.; Maeda, T.; Kobayashi, Y.; Fukazawa, Y.; Kishioka, S. Macrophage inflammatory protein-1alpha mediates the development of neuropathic pain following peripheral nerve injury through interleukin-1beta up-regulation. Pain 2010, 149, 305–315. [Google Scholar] [CrossRef]
- Zelenka, M.; Schafers, M.; Sommer, C. Intraneural injection of interleukin-1beta and tumor necrosis factor-alpha into rat sciatic nerve at physiological doses induces signs of neuropathic pain. Pain 2005, 116, 257–263. [Google Scholar] [CrossRef]
- Goh, S. Neuropathic pain—Definition and drug therapy. Aust. Prescr. 2018, 41, 178–179. [Google Scholar] [CrossRef] [PubMed]
- Baranidharan, G.; Das, S.; Bhaskar, A. A review of the high-concentration capsaicin patch and experience in its use in the management of neuropathic pain. Ther. Adv. Neurol. Disord. 2013, 6, 287–297. [Google Scholar] [CrossRef] [PubMed]
- Jones, V.M.; Moore, K.A.; Peterson, D.M. Capsaicin 8% topical patch (Qutenza)—A review of the evidence. J. Pain. Palliat. Care Pharmacother. 2011, 25, 32–41. [Google Scholar] [CrossRef]
- Murnion, B.P. Neuropathic pain: Current definition and review of drug treatment. Aust. Prescr. 2018, 41, 60–63. [Google Scholar] [CrossRef]
- Yi, M.H.; Liu, Y.U.; Liu, K.; Chen, T.; Bosco, D.B.; Zheng, J.; Xie, M.; Zhou, L.; Qu, W.; Wu, L.J. Chemogenetic manipulation of microglia inhibits neuroinflammation and neuropathic pain in mice. Brain Behav. Immun. 2021, 92, 78–89. [Google Scholar] [CrossRef] [PubMed]
- Llorca-Torralba, M.; Suárez-Pereira, I.; Bravo, L.; Camarena-Delgado, C.; Garcia-Partida, J.A.; Mico, J.A.; Berrocoso, E. Chemogenetic Silencing of the Locus Coeruleus-Basolateral Amygdala Pathway Abolishes Pain-Induced Anxiety and Enhanced Aversive Learning in Rats. Biol. Psychiatry 2019, 85, 1021–1035. [Google Scholar] [CrossRef] [PubMed]
- Saloman, J.L.; Scheff, N.N.; Snyder, L.M.; Ross, S.E.; Davis, B.M.; Gold, M.S. Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes in Sensory Neurons. J. Neurosci. 2016, 36, 10769–10781. [Google Scholar] [CrossRef]
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Hsiao, I.-H.; Yen, C.-M.; Hsu, H.-C.; Liao, H.-Y.; Lin, Y.-W. Chemogenetics Modulation of Electroacupuncture Analgesia in Mice Spared Nerve Injury-Induced Neuropathic Pain through TRPV1 Signaling Pathway. Int. J. Mol. Sci. 2024, 25, 1771. https://doi.org/10.3390/ijms25031771
Hsiao I-H, Yen C-M, Hsu H-C, Liao H-Y, Lin Y-W. Chemogenetics Modulation of Electroacupuncture Analgesia in Mice Spared Nerve Injury-Induced Neuropathic Pain through TRPV1 Signaling Pathway. International Journal of Molecular Sciences. 2024; 25(3):1771. https://doi.org/10.3390/ijms25031771
Chicago/Turabian StyleHsiao, I-Han, Chia-Ming Yen, Hsin-Cheng Hsu, Hsien-Yin Liao, and Yi-Wen Lin. 2024. "Chemogenetics Modulation of Electroacupuncture Analgesia in Mice Spared Nerve Injury-Induced Neuropathic Pain through TRPV1 Signaling Pathway" International Journal of Molecular Sciences 25, no. 3: 1771. https://doi.org/10.3390/ijms25031771