Alterations in Genome Organization in Lymphoma Cell Nuclei due to the Presence of the t(14;18) Translocation
Abstract
:1. Introduction
2. Results
2.1. t(14;18) Translocation and BCL2 Expression in the Pfeiffer Cell Line
2.2. Chromosome Territories 14 and 18 Are Unaltered in the Pfeiffer Cell Line Compared to Healthy Lymphocytes
2.3. Nuclear Positioning of BCL2, IGH, and BCL2::IGH
2.4. Gene Nuclear Positioning in Relation to Their Corresponding Chromosomal Territories
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Expression Analysis of Publicly Available Data
4.3. Control Samples
4.4. Reverse Transcription (RT) PCR
4.5. Fluorescence In Situ Hybridization (FISH) on Metaphase Chromosomes and Interphase Nuclei
4.6. Nuclear Localization Analysis
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Sequence |
---|---|
β-actin Forward | 5′-AAGAGAGGCATCCTCACCCT-3′ |
β-actin Reverse | 5′-TACATGGCTGGGGTGTTGAA-3′ |
Bcl-2 Forward | 5′-ACGGAGGCTGGGATGCCTTTG-3′ |
Bcl-2 Reverse | 5‘-ACACGAAGCGGTGCTTGGCA-3′ |
Probe Name | Vector Type | Length, bp | Locus | Position | Labelling |
---|---|---|---|---|---|
RP5-998D24 | PAC | 165,821 | IGH | 14q32 | Digoxigenin-FITC |
RP11-299P2 | BAC | 146,688 | BCL2 | 18q21 | Biotin-Texas Red |
WCP14 * | Paint | Chr 14 | Biotin-Texas Red | ||
WCP18 * | Paint | Chr 18 | FITC |
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Garimberti, E.; Federico, C.; Ragusa, D.; Bruno, F.; Saccone, S.; Bridger, J.M.; Tosi, S. Alterations in Genome Organization in Lymphoma Cell Nuclei due to the Presence of the t(14;18) Translocation. Int. J. Mol. Sci. 2024, 25, 2377. https://doi.org/10.3390/ijms25042377
Garimberti E, Federico C, Ragusa D, Bruno F, Saccone S, Bridger JM, Tosi S. Alterations in Genome Organization in Lymphoma Cell Nuclei due to the Presence of the t(14;18) Translocation. International Journal of Molecular Sciences. 2024; 25(4):2377. https://doi.org/10.3390/ijms25042377
Chicago/Turabian StyleGarimberti, Elisa, Concetta Federico, Denise Ragusa, Francesca Bruno, Salvatore Saccone, Joanna Mary Bridger, and Sabrina Tosi. 2024. "Alterations in Genome Organization in Lymphoma Cell Nuclei due to the Presence of the t(14;18) Translocation" International Journal of Molecular Sciences 25, no. 4: 2377. https://doi.org/10.3390/ijms25042377