Gut Microbiota and Inflammation Modulation in a Rat Model for Ulcerative Colitis after the Intraperitoneal Administration of Apigenin, Luteolin, and Xanthohumol
Abstract
:1. Introduction
2. Results
2.1. Effect of Flavonoid Treatments on Body Weight and DAI
2.2. Effect of Flavonoid Treatments on Hyperplastic Peyer’s Patches, Colon Length, and Colon Ulceration
2.3. Effect of Flavonoid Treatments on Pro-Inflammatory Cytokines and Myeloperoxidase (MPO)
2.4. Caecum Weight and Metataxonomic Analyses of the Gut Microbiota
3. Discussion
4. Materials and Methods
4.1. Drugs and Chemicals
4.2. Animals
4.3. Experimental Design
4.4. Tissue Sample Collection
4.5. Histological Studies
4.6. Assessment of the Disease Activity Index (DAI)
4.7. Pro-Inflammatory Cytokine Analysis in Plasma
4.8. Myeloperoxidase (MPO) Assays
4.9. 16S rRNA Sequencing and Gut Microbiota Analysis
4.10. Microbiota Analysis
4.11. Statistical Methods
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Phylum | PBS | Apigenin | Luteolin | Xanthohumol | PBS vs. API | PBS vs. LUT | PBS vs. XAN |
---|---|---|---|---|---|---|---|
Actinomycetota | 1.65 | 1.31 | 0.83 | 1.45 | |||
Bacteroidota | 40.05 | 44.32 | 39.40 | 34.99 | * | ||
Deferribacterota | 0.46 | 0.29 | 0.33 | 0.35 | |||
Desulfobacterota | 0.36 | 0.53 | 0.07 | 0.03 | * | ||
Bacillota | 51.20 | 45.49 | 51.48 | 52.32 | * | ||
Pseudomonadota | 1.28 | 1.87 | 0.23 | 0.17 | ** | *** | |
Verrucomicrobiota | 4.56 | 5.57 | 6.80 | 9.77 | * |
Family | PBS | Apigenin | Luteolin | Xanthohumol | PBS vs. API | PBS vs. LUT | PBS vs. XAN |
---|---|---|---|---|---|---|---|
Bifidobacteriaceae | 0.52 | 0.43 | 0.03 | 0.35 | ** | ||
Atopobiaceae | 0.42 | 0.43 | 0.007 | 0.18 | ** | ||
Eggerthellaceae | 0.68 | 0.43 | 0.79 | 0.89 | |||
Bacteroidaceae | 12.66 | 16.23 | 10.59 | 15.25 | |||
Muribaculaceae | 14.15 | 13.16 | 17.56 | 12.16 | |||
Prevotellaceae | 1.73 | 2.48 | 2.38 | 3.83 | * | ||
Rikenellaceae | 7.76 | 5.71 | 6.39 | 0.46 | **** | ||
Tannerellaceae | 3.74 | 6.51 | 2.33 | 3.22 | |||
Deferribacteraceae | 0.46 | 0.29 | 0.33 | 0.35 | |||
Desulfovibrionaceae | 0.36 | 0.53 | 0.07 | 0.03 | * | ||
Bacillaceae | 0.45 | 0.29 | 0.37 | 0.13 | |||
Erysipelatoclostridiaceae | 0.79 | 1.09 | 1.20 | 0.57 | |||
Erysipelotrichaceae | 2.08 | 0.53 | 0.38 | 0.36 | * | ** | ** |
Lactobacillaceae | 7.08 | 5.77 | 8.04 | 9.31 | |||
Streptococcaceae | 0.16 | 0.02 | 0.003 | 0.02 | * | *** | * |
Staphylococcaceae | 0.35 | 0.05 | 0 | 0.003 | ** | *** | **** |
Christensenellaceae | 0.36 | 0.10 | 0.08 | 1.07 | * | * | |
Clostridia UCG-014 | 1.57 | 1.30 | 3.07 | 5.10 | ** | **** | |
Clostridiaceae | 0.4 | 0.43 | 0.11 | 0.12 | *** | ** | |
Lachnospiraceae | 14.96 | 15.65 | 23.25 | 19.75 | ** | ||
Monoglobaceae | 1.08 | 0.59 | 0.71 | 1.41 | |||
Oscillospiraceae | 4.63 | 6.21 | 4.92 | 3.89 | |||
Ruminococcaceae | 12.28 | 9.39 | 7.38 | 7.93 | |||
Eubacterium | 0.75 | 0.58 | 0.93 | 0.97 | |||
Peptococcaceae | 0.39 | 0.63 | 0.35 | 0.17 | ** | ||
Anaerovoracaceae | 0.33 | 0.29 | 0.13 | 0.11 | ** | *** | |
Peptostreptococcaceae | 2.88 | 1.96 | 0.21 | 0.86 | **** | * | |
Sutterellaceae | 0.54 | 0.45 | 0.11 | 0.08 | ** | **** | |
Enterobacteriaceae | 0.68 | 1.30 | 0.05 | 0.03 | ** | ** | |
Akkermansiaceae | 4.56 | 5.57 | 6.80 | 9.77 | * |
Genus Species | PBS | Apigenin | Luteolin | Xanthohumol | PBS vs. API | PBS vs. LUT | PBS vs. XAN |
---|---|---|---|---|---|---|---|
Bifidobacterium | 0.52 | 0.43 | 0.03 | 0.35 | ** | ||
B. animalis | 0.41 | 0.41 | 0.03 | 0.28 | * | ||
Adlercreutzia | 0.40 | 0.22 | 0.17 | 0.24 | * | ||
Enterorhabdus | 0.17 | 0.15 | 0.55 | 0.46 | **** | *** | |
Bacteroides | 12.66 | 16.23 | 10.59 | 15.25 | |||
B. dorei | 0.87 | 1.59 | 0.36 | 1.57 | * | ** | * |
B. thetaiotaomicron | 1.31 | 2.09 | 1.12 | 2.32 | * | ||
Alistipes | 7.66 | 5.67 | 6.37 | 0.39 | **** | ||
Bilophila | 0.36 | 0.53 | 0.07 | 0.03 | * | ||
Turicibacter | 0.85 | 0.35 | 0.10 | 0.22 | **** | * | |
Streptococcus | 0.15 | 0.02 | 0.003 | 0.016 | ** | * | |
Staphylococcus | 0.35 | 0.05 | 0 | 0.003 | *** | *** | **** |
Clostridia_UCG-014 | 1.57 | 1.30 | 3.07 | 5.10 | ** | **** | |
Clostridium sensu stricto 1 | 0.40 | 0.43 | 0.11 | 0.12 | *** | ** | |
Blautia | 0.07 | 0.30 | 0.37 | 0.19 | * | * | |
Uncharacterized | 0.01 | 0.28 | 0.33 | 0.15 | ** | ** | * |
Lachnospiraceae NK4A136 | 6.19 | 7.32 | 15.48 | 12.27 | * | ||
Ruminococcus | 6.19 | 3.47 | 1.48 | 2.93 | ** | ||
Peptococacceae uncultured | 0.36 | 0.53 | 0.31 | 0.17 | *** | ||
Romboutsia | 2.88 | 1.96 | 0.21 | 0.86 | **** | * | |
Parasutterella | 0.54 | 0.45 | 0.11 | 0.08 | ** | **** | |
Escherichia-Shigella | 0.68 | 1.30 | 0.05 | 0.03 | ** | ** | |
Akkermansia | 4.56 | 5.57 | 6.80 | 9.77 | * | ||
A. muciniphila | 4.56 | 5.57 | 6.80 | 9.77 | * |
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Magadán-Corpas, P.; Pérez-Valero, Á.; Ye, S.; Sordon, S.; Huszcza, E.; Popłoński, J.; Villar, C.J.; Lombó, F. Gut Microbiota and Inflammation Modulation in a Rat Model for Ulcerative Colitis after the Intraperitoneal Administration of Apigenin, Luteolin, and Xanthohumol. Int. J. Mol. Sci. 2024, 25, 3236. https://doi.org/10.3390/ijms25063236
Magadán-Corpas P, Pérez-Valero Á, Ye S, Sordon S, Huszcza E, Popłoński J, Villar CJ, Lombó F. Gut Microbiota and Inflammation Modulation in a Rat Model for Ulcerative Colitis after the Intraperitoneal Administration of Apigenin, Luteolin, and Xanthohumol. International Journal of Molecular Sciences. 2024; 25(6):3236. https://doi.org/10.3390/ijms25063236
Chicago/Turabian StyleMagadán-Corpas, Patricia, Álvaro Pérez-Valero, Suhui Ye, Sandra Sordon, Ewa Huszcza, Jarosław Popłoński, Claudio J. Villar, and Felipe Lombó. 2024. "Gut Microbiota and Inflammation Modulation in a Rat Model for Ulcerative Colitis after the Intraperitoneal Administration of Apigenin, Luteolin, and Xanthohumol" International Journal of Molecular Sciences 25, no. 6: 3236. https://doi.org/10.3390/ijms25063236