StMAPKK5 Positively Regulates Response to Drought and Salt Stress in Potato
Abstract
:1. Introduction
2. Results
2.1. Analysis of Tissue Specificity and Expression Pattern of StMAPKK5 in Potato
2.2. Subcellular Localization Assay
2.3. Genetic Transformation of StMAPKK5 and Identification of Transgenic Potato Plants
2.4. StMAPKK5 Positively Regulates Drought Resistance and Salt Tolerance of Potato
2.5. Yeast Two-Hybridization to Identify StMAPKK5-Interacting Proteins
3. Discussion
4. Materials and Methods
4.1. Growth Conditions and Treatment of Plant Materials
4.2. Cloning of StMAPKK5
4.3. StMAPKK5 Expression Analysis by qRT-PCR
4.4. Subcellular Localization of StMAPKK5
4.5. Construction of Plant Expression Vectors
4.6. Genetic Transformation of Potatoes
4.7. Drought and NaCl Stress Treatment
4.8. Yeast Two-Hybrid Assay
4.9. Bimolecular Fluorescence Complementation Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Luo, Y.; Wang, K.; Zhu, L.; Zhang, N.; Si, H. StMAPKK5 Positively Regulates Response to Drought and Salt Stress in Potato. Int. J. Mol. Sci. 2024, 25, 3662. https://doi.org/10.3390/ijms25073662
Luo Y, Wang K, Zhu L, Zhang N, Si H. StMAPKK5 Positively Regulates Response to Drought and Salt Stress in Potato. International Journal of Molecular Sciences. 2024; 25(7):3662. https://doi.org/10.3390/ijms25073662
Chicago/Turabian StyleLuo, Yu, Kaitong Wang, Liping Zhu, Ning Zhang, and Huaijun Si. 2024. "StMAPKK5 Positively Regulates Response to Drought and Salt Stress in Potato" International Journal of Molecular Sciences 25, no. 7: 3662. https://doi.org/10.3390/ijms25073662