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Article

Hyperbaric Oxygen Reduces Oxidative Stress Impairment and DNA Damage and Simultaneously Increases HIF-1α in Ischemia–Reperfusion Acute Kidney Injury

by
Jelena Nesovic Ostojic
1,*,†,
Sanjin Kovacevic
1,†,
Milan Ivanov
2,
Predrag Brkic
3,
Maja Zivotic
4,
Nevena Mihailovic-Stanojevic
2,
Danijela Karanovic
2,
Una Jovana Vajic
2,
Rada Jeremic
3,
Djurdjica Jovovic
2 and
Zoran Miloradovic
2
1
Department of Pathological Physiology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia
2
Department of Cardiovascular Physiology, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia
3
Department of Medical Physiology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia
4
Institute of Pathology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(7), 3870; https://doi.org/10.3390/ijms25073870
Submission received: 31 January 2024 / Revised: 25 March 2024 / Accepted: 27 March 2024 / Published: 30 March 2024
(This article belongs to the Special Issue Oxygen Variations, 2nd Edition)
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Abstract

The central exacerbating factor in the pathophysiology of ischemic–reperfusion acute kidney injury (AKI) is oxidative stress. Lipid peroxidation and DNA damage in ischemia are accompanied by the formation of 3-nitrotyrosine, a biomarker for oxidative damage. DNA double-strand breaks (DSBs) may also be a result of postischemic AKI. γH2AX(S139) histone has been identified as a potentially useful biomarker of DNA DSBs. On the other hand, hypoxia-inducible factor (HIF) is the “master switch” for hypoxic adaptation in cells and tissues. The aim of this research was to evaluate the influence of hyperbaric oxygen (HBO) preconditioning on antioxidant capacity estimated by FRAP (ferric reducing antioxidant power) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assay, as well as on oxidative stress parameter 3-nitrotyrosine, and to assess its effects on γH2AX(S139), HIF-1α, and nuclear factor-κB (NF-κB) expression, in an experimental model of postischemic AKI induced in spontaneously hypertensive rats. The animals were divided randomly into three experimental groups: sham-operated rats (SHAM, n = 6), rats with induced postischemic AKI (AKI, n = 6), and group exposed to HBO preconditioning before AKI induction (AKI + HBO, n = 6). A significant improvement in the estimated glomerular filtration rate, eGFR, in AKI + HBO group (p < 0.05 vs. AKI group) was accompanied with a significant increase in plasma antioxidant capacity estimated by FRAP (p < 0.05 vs. SHAM group) and a reduced immunohistochemical expression of 3-nitrotyrosine and γH2AX(S139). Also, HBO pretreatment significantly increased HIF-1α expression (p < 0.001 vs. AKI group), estimated by Western blot and immunohistochemical analysis in kidney tissue, and decreased immunohistochemical NF-κB renal expression (p < 0.01). Taking all of these results together, we may conclude that HBO preconditioning has beneficial effects on acute kidney injury induced in spontaneously hypertensive rats.
Keywords: oxidative stress; FRAP; 3-nitrotyrosine; γH2AX(S139); HIF-1α; NF-κB; spontaneously hypertensive rats; HBO preconditioning oxidative stress; FRAP; 3-nitrotyrosine; γH2AX(S139); HIF-1α; NF-κB; spontaneously hypertensive rats; HBO preconditioning

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MDPI and ACS Style

Nesovic Ostojic, J.; Kovacevic, S.; Ivanov, M.; Brkic, P.; Zivotic, M.; Mihailovic-Stanojevic, N.; Karanovic, D.; Vajic, U.J.; Jeremic, R.; Jovovic, D.; et al. Hyperbaric Oxygen Reduces Oxidative Stress Impairment and DNA Damage and Simultaneously Increases HIF-1α in Ischemia–Reperfusion Acute Kidney Injury. Int. J. Mol. Sci. 2024, 25, 3870. https://doi.org/10.3390/ijms25073870

AMA Style

Nesovic Ostojic J, Kovacevic S, Ivanov M, Brkic P, Zivotic M, Mihailovic-Stanojevic N, Karanovic D, Vajic UJ, Jeremic R, Jovovic D, et al. Hyperbaric Oxygen Reduces Oxidative Stress Impairment and DNA Damage and Simultaneously Increases HIF-1α in Ischemia–Reperfusion Acute Kidney Injury. International Journal of Molecular Sciences. 2024; 25(7):3870. https://doi.org/10.3390/ijms25073870

Chicago/Turabian Style

Nesovic Ostojic, Jelena, Sanjin Kovacevic, Milan Ivanov, Predrag Brkic, Maja Zivotic, Nevena Mihailovic-Stanojevic, Danijela Karanovic, Una Jovana Vajic, Rada Jeremic, Djurdjica Jovovic, and et al. 2024. "Hyperbaric Oxygen Reduces Oxidative Stress Impairment and DNA Damage and Simultaneously Increases HIF-1α in Ischemia–Reperfusion Acute Kidney Injury" International Journal of Molecular Sciences 25, no. 7: 3870. https://doi.org/10.3390/ijms25073870

APA Style

Nesovic Ostojic, J., Kovacevic, S., Ivanov, M., Brkic, P., Zivotic, M., Mihailovic-Stanojevic, N., Karanovic, D., Vajic, U. J., Jeremic, R., Jovovic, D., & Miloradovic, Z. (2024). Hyperbaric Oxygen Reduces Oxidative Stress Impairment and DNA Damage and Simultaneously Increases HIF-1α in Ischemia–Reperfusion Acute Kidney Injury. International Journal of Molecular Sciences, 25(7), 3870. https://doi.org/10.3390/ijms25073870

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