Integrative Metabolomic and Transcriptomic Analyses Reveal the Mechanism of Petal Blotch Formation in Rosa persica
Abstract
:1. Introduction
2. Results
2.1. Metabolomic Differences among R. persica Petals
2.2. Differential Accumulation of Carotenoids in the Petals of R. persica
2.3. Transcriptomic Analysis of R. persica
2.4. Differentially Expressed Gene (DEG) Analysis of Petal Blotch and Non-Blotch Parts at Four Stages
2.5. Expression Profiles of Genes Related to Anthocyanin Biosynthesis Involved in Blotch Formation
2.6. Identification of TFs Involved in Blotch Formation
2.7. qRT-PCR Validation of DEGs
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Metabolomic Analysis of Flavonoids and Carotenoids
4.3. Transcriptome Sequencing and Analyses
4.4. Weighted Gene Co-Expression Network Analysis
4.5. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) Analysis
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, H.; Kong, Y.; Dou, X.; Yang, Y.; Chi, X.; Lang, L.; Zhang, Q.; Pan, H.; Bai, J. Integrative Metabolomic and Transcriptomic Analyses Reveal the Mechanism of Petal Blotch Formation in Rosa persica. Int. J. Mol. Sci. 2024, 25, 4030. https://doi.org/10.3390/ijms25074030
Wang H, Kong Y, Dou X, Yang Y, Chi X, Lang L, Zhang Q, Pan H, Bai J. Integrative Metabolomic and Transcriptomic Analyses Reveal the Mechanism of Petal Blotch Formation in Rosa persica. International Journal of Molecular Sciences. 2024; 25(7):4030. https://doi.org/10.3390/ijms25074030
Chicago/Turabian StyleWang, Huan, Ying Kong, Xiaoying Dou, Yi Yang, Xiufeng Chi, Lixin Lang, Qixiang Zhang, Huitang Pan, and Jinrong Bai. 2024. "Integrative Metabolomic and Transcriptomic Analyses Reveal the Mechanism of Petal Blotch Formation in Rosa persica" International Journal of Molecular Sciences 25, no. 7: 4030. https://doi.org/10.3390/ijms25074030