MyoD Over-Expression Rescues GST-bFGF Repressed Myogenesis
Abstract
:1. Introduction
2. Results
2.1. Recombinant GST-FGF Induces Akt and MAPK Signalling in Myoblasts
2.2. GST-bFGF Is a Strong Proliferation Inducer of Myoblasts
2.3. GST-bFGF Reversibly Repressed Myogenic Differentiation
2.4. GST-bFGF Induces Cell Cycle Genes but Selectively Represses Myogenic Genes
2.5. MyoD Repression Is Dispensable for GST-bFGF Induced Cell Proliferation
2.6. GST-bFGF Signaling Enhances the Stemness of Satellite Cells
3. Discussion
3.1. GST-bFGF Conserves the Property of Native bFGF
3.2. Differential Effects on Free and Myofiber-Associated Satellite Cells
3.3. MyoD Is the Major Target of bFGF in Myogenic Repression
4. Materials and Methods
4.1. Plasmids
4.2. Cell Culture and Promoter Assay
4.3. Recombinant protein Expression and Purification
4.4. Quantitative RT-PCR (qRT-PCR)
4.5. Western Blot
4.6. Satellite Cell Isolation and Culture
4.7. Flowcytometry
4.8. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fan, S.-H.; Li, N.; Huang, K.-F.; Chang, Y.-T.; Wu, C.-C.; Chen, S.-L. MyoD Over-Expression Rescues GST-bFGF Repressed Myogenesis. Int. J. Mol. Sci. 2024, 25, 4308. https://doi.org/10.3390/ijms25084308
Fan S-H, Li N, Huang K-F, Chang Y-T, Wu C-C, Chen S-L. MyoD Over-Expression Rescues GST-bFGF Repressed Myogenesis. International Journal of Molecular Sciences. 2024; 25(8):4308. https://doi.org/10.3390/ijms25084308
Chicago/Turabian StyleFan, Shu-Hsin, Ning Li, Kai-Fan Huang, Yun-Ting Chang, Chuan-Che Wu, and Shen-Liang Chen. 2024. "MyoD Over-Expression Rescues GST-bFGF Repressed Myogenesis" International Journal of Molecular Sciences 25, no. 8: 4308. https://doi.org/10.3390/ijms25084308