Molecular Epidemiology of Carbapenem-Resistant Klebsiella aerogenes in Japan
Abstract
:1. Introduction
2. Results
2.1. Carbapenem-Resistant Klebsiella aerogenes Isolates across Medical Facilities in Japan
2.2. PCR, Whole-Genome Analysis, and MLST
2.3. MLST and Phylogenetic Analysis of Carbapenemase-Producing Klebsiella aerogenes
2.4. Plasmid Type (Inc Genes)
2.5. Integron Structure Harboring blaIMP
2.6. Transposons Containing blaNDM-1 and blaNDM-5 on IncX3-Type Plasmid
3. Discussion
3.1. Global and Local Perspectives on Carbapenemase Presence in Klebsiella aerogenes
3.2. Sequence Types and Carbapenemase Variants in K. aerogenes
3.3. Mobile Genetic Elements in This Study, Including Plasmids, Integrons, and Transposons
4. Materials and Methods
4.1. Bacterial Strains
4.2. Screening of Carbapenemase Genes by PCR
4.3. Whole-Genome Sequencing and Genomic Analysis
4.4. MLST and Phylogenetic Analysis of Carbapenemase-Producing K. aerogenes
4.5. Analysis of Plasmids, Integrons, and Transposons Associated with Carbapenemase
4.6. Data Availability
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain Name | Year of Isolation | Prefecture | Type of Facility | Age (Sex) | Sample | STs | Carbapenemase Gene Type | Replicon Types | Integron and Transposon | Antimicrobial Resistance Genes Except Carbapenemase Gene |
---|---|---|---|---|---|---|---|---|---|---|
TUT0013 | 2016 | Fukuoka | Acute | 89 (M) | Bile | 189 | blaIMP-6 | – | In1690 | aadA2, aac(6′)-Ib, sul1, blaSHV-190, oqxA, tet(A), blaCTX-M-2 |
TUT0015 | 2016 | Fukuoka | Long-term | 87 (F) | Sputum | 4 | blaIMP-1 | IncN4 | Novel class 1 integron | blaOXA-101, aph(3′)-XV, fosA, qnrS1 |
TUT0023 | 2017 | Osaka | Acute | 67 (M) | Urine | 668 | blaIMP-6 | – | In1547-like | aadA2, aac(6′)-Ib, sul1, fosA7, dfrA14, blaCTX-M-2 |
TUT0032 | 2018 | Osaka | Acute | 84 (F) | Sputum | 668 | blaIMP-6 | – | In1547 | aadA2, aac(6′)-Ib, sul1, fosA7, dfrA14, blaCTX-M-2 |
TUT0039 | 2018 | Ishikawa | Acute | 72 (M) | Urine | 709 | blaNDM-5 | IncX3 | Tn3000-like | qnrD1 |
TUT0049 | 2019 | Kanagawa | Acute | 72 (F) | Vaginal discharge | 296 | blaNDM-1 | IncX3 | Tn3000-like | qnrD1 |
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Takei, K.; Ogawa, M.; Sakata, R.; Kanamori, H. Molecular Epidemiology of Carbapenem-Resistant Klebsiella aerogenes in Japan. Int. J. Mol. Sci. 2024, 25, 4494. https://doi.org/10.3390/ijms25084494
Takei K, Ogawa M, Sakata R, Kanamori H. Molecular Epidemiology of Carbapenem-Resistant Klebsiella aerogenes in Japan. International Journal of Molecular Sciences. 2024; 25(8):4494. https://doi.org/10.3390/ijms25084494
Chicago/Turabian StyleTakei, Kentarou, Miho Ogawa, Ryuji Sakata, and Hajime Kanamori. 2024. "Molecular Epidemiology of Carbapenem-Resistant Klebsiella aerogenes in Japan" International Journal of Molecular Sciences 25, no. 8: 4494. https://doi.org/10.3390/ijms25084494