Comparison of GFAP and UCH-L1 Measurements Using Two Automated Immunoassays (i-STAT® and Alinity®) for the Management of Patients with Mild Traumatic Brain Injury: Preliminary Results from a French Single-Center Approach
Abstract
:1. Introduction
2. Results
2.1. Patient Characteristics
2.2. Analytical Correlation and Concordance
2.2.1. GFAP
2.2.2. UCH-L1
2.3. Biomarker Levels According to Imaging Findings on CT-Scan
2.3.1. i-STAT® Analyzer
2.3.2. Alinity® i Analyzer
2.4. Clinical Concordance for the Combined Test “GFAP + UCH-L1”
3. Discussion
4. Materials and Methods
4.1. Study Design and Patients
4.2. GFAP and UCH-L1 Assays
4.2.1. i-STAT® Analyzer
4.2.2. Alinity® Analyzer
4.3. Cranial Computed Tomography Scan
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Demographic Characteristics and Clinically Relevant Information | |
---|---|
Total | 230 |
Sex ratio (M/F) | 1.2 |
Mean age in years (min; max; IQR) | 66.2 (18.2; 101.2; 33.9–82.8) |
Mean sampling time in minutes (min; max; IQR) | 100.5 (25; 720; 75–162) |
GCS at admission, n (%) | |
13 | 1 (0.3) |
14 | 9 (4) |
15 | 220 (95.7) |
Clinical outcome, n (%) | |
Discharged | 194 (84.3) |
Hospitalized for surveillance | 35 (15.3) |
Death | 1 (0.4) |
CT+ patients, n (%) | |
Subdural hematoma | 5 (2.2) |
Subarachnoid hemorrhage | 6 (2.6) |
GFAP | UCH-L1 | |
---|---|---|
i-STAT® (ng/L), median | 43 | 282 |
(min; max; IQR) | (30; 907; 30–76) | (200; 2376; 200–472) |
Alinity® i (ng/L), median | 46.3 | 350 |
(min; max; IQR) | (1; 961; 23–85) | (42; 2940; 200–548) |
p-Value | 0.17 | 0.14 |
Lin (95% CI) | 0.93 (0.91–0.95) | 0.89 (0.87–0.92) |
% of agreement | 94 | 86 |
Kappa (95% CI) | 0.88 (0.82–0.94) | 0.70 (0.60–0.79) |
i-STAT® | |||
ICL− (n = 219) | ICL+ (n = 11) | p-Value | |
GFAP in ng/L median (min; max; IQR) | 41 (30; 507; 30–74) | 85 (30; 907; 59–179) | 0.003 |
UCH-L1 in ng/L median (min; max; IQR) | 279 (200; 2376; 200–471) | 312 (200; 1931; 281–540) | 0.20 |
Alinity® i | |||
ICL− (n = 219) | ICL+ (n = 11) | p-Value | |
GFAP in ng/L median (min; max; IQR) | 44 (1;546; 23–83) | 87 (29; 961; 71.1–165) | 0.005 |
UCH-L1 in ng/L median (min; max; IQR) | 342 (42; 2940; 197–548) | 469 (187; 1603; 282–570) | 0.14 |
GFAP + UCH-L1 (i-STAT®) | GFAP + UCH-L1 (Alinity® i) | p-Value | |
---|---|---|---|
SE (95% CI) | 100% (75.1–100%) | 100% (72–100%) | 1 |
SP (95% CI) | 28.8% (22.9–35.3%) | 29.7% (23.7–36.2%) | 0.68 |
PPV (95% CI) | 6.6% (3.3–11.5%) | 6.7% (3.3–11.5%) | 0.98 |
NPV (95% CI) | 100% (94.3–100%) | 100% (94.5–100%) | 1 |
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Oris, C.; Khatib-Chahidi, C.; Pereira, B.; Bailly Defrance, V.; Bouvier, D.; Sapin, V. Comparison of GFAP and UCH-L1 Measurements Using Two Automated Immunoassays (i-STAT® and Alinity®) for the Management of Patients with Mild Traumatic Brain Injury: Preliminary Results from a French Single-Center Approach. Int. J. Mol. Sci. 2024, 25, 4539. https://doi.org/10.3390/ijms25084539
Oris C, Khatib-Chahidi C, Pereira B, Bailly Defrance V, Bouvier D, Sapin V. Comparison of GFAP and UCH-L1 Measurements Using Two Automated Immunoassays (i-STAT® and Alinity®) for the Management of Patients with Mild Traumatic Brain Injury: Preliminary Results from a French Single-Center Approach. International Journal of Molecular Sciences. 2024; 25(8):4539. https://doi.org/10.3390/ijms25084539
Chicago/Turabian StyleOris, Charlotte, Clara Khatib-Chahidi, Bruno Pereira, Valentin Bailly Defrance, Damien Bouvier, and Vincent Sapin. 2024. "Comparison of GFAP and UCH-L1 Measurements Using Two Automated Immunoassays (i-STAT® and Alinity®) for the Management of Patients with Mild Traumatic Brain Injury: Preliminary Results from a French Single-Center Approach" International Journal of Molecular Sciences 25, no. 8: 4539. https://doi.org/10.3390/ijms25084539