Verification of AKT and CDK5 Gene and RNA Interference Combined with Irradiation to Mediate Fertility Changes in Plutella xylostella (Linnaeus)
Abstract
:1. Introduction
2. Results
2.1. The Effects on the Expression Levels of Nine Genes of Irradiation
2.2. The Effects on Plutella xylostella Reproductive Indicators of Gene-Knockdown
2.3. The Effects of PxAKT and PxCDK5 Knockdown on Plutella xylostella Sperm Bundles
2.4. The Alterations in PxAKT and PxCDK5 Expression Levels and in the Fertility of Plutella xylostella across Generations Following Irradiation Treatment
2.5. The Impact of PxAKT Injection Combined with Low-Dose Irradiation on Plutella xylostella Fertility
2.6. The Effects of Low-Dose Irradiation Combined with PxCDK5 Knockdown on the Fertility of Plutella xylostella
3. Discussion
4. Materials and Methods
4.1. Insect Rearing
4.2. 60Co-γ Irradiation of Male Pupae
4.3. Bioassay of the Effects on Fecundity and Vitality
4.3.1. Oviposition and Egg Hatchability
4.3.2. Pupation Rate, Emergence Rate and Adult Lifespan
4.4. Dissection and Observation of Testes
4.5. Measurements of the Number of Sperm Bundles and the Weight of the Testes
4.6. Total RNA Extraction and cDNA Synthesis
4.7. Quantitative Real-Time PCR (qRT-PCR) Analysis
4.8. RNA Interference, Including dsRNA Synthesis and dsRNA Injecting
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Wen, J.; Wang, M.; Zeng, Y.; He, F.; Li, S.; Zhang, K.; Weng, Q. Verification of AKT and CDK5 Gene and RNA Interference Combined with Irradiation to Mediate Fertility Changes in Plutella xylostella (Linnaeus). Int. J. Mol. Sci. 2024, 25, 4623. https://doi.org/10.3390/ijms25094623
Wen J, Wang M, Zeng Y, He F, Li S, Zhang K, Weng Q. Verification of AKT and CDK5 Gene and RNA Interference Combined with Irradiation to Mediate Fertility Changes in Plutella xylostella (Linnaeus). International Journal of Molecular Sciences. 2024; 25(9):4623. https://doi.org/10.3390/ijms25094623
Chicago/Turabian StyleWen, Jiaqi, Mengran Wang, Yuhao Zeng, Fengting He, Shifan Li, Ke Zhang, and Qunfang Weng. 2024. "Verification of AKT and CDK5 Gene and RNA Interference Combined with Irradiation to Mediate Fertility Changes in Plutella xylostella (Linnaeus)" International Journal of Molecular Sciences 25, no. 9: 4623. https://doi.org/10.3390/ijms25094623