Anti-Photodamage Effect of Agaricus blazei Murill Polysaccharide on UVB-Damaged HaCaT Cells
Abstract
:1. Introduction
2. Results
2.1. Effects of ABP on Cell Viability, Inflammatory Cytokine Secretion, and Skin Barrier Function
2.2. Isolation, Purification, and Structural Analysis
2.3. Effects of ABP Components A1 and A2 on Cell Viability and Proliferation
2.4. Effects of A1 and A2 on Mitochondrial Membrane Potential (ΔΨm)
2.5. Effects of A1 and A2 on the Expression Levels of Inflammatory Factors
2.6. Influences of A1 and A2 on MMP-1, MMP-9, ELN, and COX-2
2.7. Expression of JAK-STAT Pathway-Related Genes
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Extraction, Separation, and Purification of ABP
4.3. UV–Vis
4.4. FT-IR
4.5. SEM
4.6. AFM
4.7. GPC and Monosaccharide Composition Analysis
4.8. Cell Culture and Toxicity Assay
4.9. The Secretions of Inflammatory Factors and Related Enzymes Were Determined by ELISA
4.10. Determination of Cell Migration Capacity
4.11. Mitochondrial Membrane Potential Measurements
4.12. RT-qPCR
4.13. Immunofluorescence Analysis
4.14. Western Blot
4.15. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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A1 | A2 | |
---|---|---|
Peak limits (min) | 21.921–24.953 | 25.237–26.727 |
Mw | 1.5 × 104 | 6.5 × 104 |
Mz | 2.4 × 104 | 9.1 × 104 |
Mw/Mn | 1.282 | 1.297 |
Mz/Mn | 2.011 | 1.818 |
Monosaccharide Ratio (%) | A1 | A2 |
---|---|---|
Mannose | 3.918 | 0.640 |
Ribose | 0.976 | 0.191 |
Rhamnose | 3.077 | 0.716 |
Glucuronic acid | 1.353 | 0.285 |
Galacturonic acid | 0.617 | 0.571 |
Glucose | 61.478 | 78.373 |
Galactose | 21.460 | 11.108 |
Xylose | —— | 0.977 |
Arabinose | 5.832 | 6.925 |
Fucose | 1.290 | 0.215 |
Total | 100.000 | 100.000 |
Peak No. | RT | Standard Sugar | Molecular Weight | Standard Curve * |
---|---|---|---|---|
1 | 15.949 | Mannose | 180.16 | y = 29,549.99x |
2 | 20.586 | Ribose | 150.13 | y = 35,195.08x |
3 | 21.669 | Rhamnose | 164.16 | y = 24,606.19x |
4 | 26.147 | Glucuronic acid | 194.14 | y = 23,994.21x |
5 | 30.011 | Galacturonic acid | 194.14 | y = 25,574.43x |
6 | 31.584 | N-acetyl-glucosamine | 221.21 | y = 21,259.33x |
7 | 34.458 | Glucose | 180.16 | y = 28,273.87x |
8 | 36.462 | N-acetyl-galactose | 221.21 | y = 27,966.82x |
9 | 39.481 | Galactose | 180.16 | y = 33,401.43x |
10 | 41.267 | Xylose | 150.13 | y = 37,008.62x |
11 | 42.925 | Arabinose | 150.13 | y = 40,595.71x |
12 | 48.109 | Fucose | 164.16 | y = 34,153.37x |
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Cheng, W.; Di, F.; Li, L.; Pu, C.; Wang, C.; Zhang, J. Anti-Photodamage Effect of Agaricus blazei Murill Polysaccharide on UVB-Damaged HaCaT Cells. Int. J. Mol. Sci. 2024, 25, 4676. https://doi.org/10.3390/ijms25094676
Cheng W, Di F, Li L, Pu C, Wang C, Zhang J. Anti-Photodamage Effect of Agaricus blazei Murill Polysaccharide on UVB-Damaged HaCaT Cells. International Journal of Molecular Sciences. 2024; 25(9):4676. https://doi.org/10.3390/ijms25094676
Chicago/Turabian StyleCheng, Wenjing, Feiqian Di, Luyao Li, Chunhong Pu, Changtao Wang, and Jiachan Zhang. 2024. "Anti-Photodamage Effect of Agaricus blazei Murill Polysaccharide on UVB-Damaged HaCaT Cells" International Journal of Molecular Sciences 25, no. 9: 4676. https://doi.org/10.3390/ijms25094676