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Article

Bioactive Phenolics of Hyoscyamus muticus L. Subsp. Falezlez: A Molecular and Biochemical Approach to Antioxidant and Urease Inhibitory Activities

by
Sabrina Lekmine
1,*,
Ouided Benslama
2,
Bachir Bensalah
3,
Nabil Touzout
4,
Hamza Moussa
5,6,
Hichem Tahraoui
4,7,8,
Mohammad Shamsul Ola
9,
Haroun Hafsa
10,
Jie Zhang
11 and
Abdeltif Amrane
8
1
Biotechnology, Water, Environment and Health Laboratory, Abbes Laghrour University, Khenchela 40000, Algeria
2
Laboratory of Natural Substances, Biomolecules, and Biotechnological Applications, Department of Natural and Life Sciences, Larbi Ben M’Hidi University, Oum El Bouaghi 04000, Algeria
3
Department of Biology, Faculty of Natural and Life Sciences, University of Ghardaia, Ghardaia 47000, Algeria
4
Laboratory of Biomaterials and Transport Phenomena (LBMTP), University Yahia Fares, Médéa 26000, Algeria
5
Laboratoire de Gestion et Valorisation des Ressources Naturelles et Assurance Qualité (LGVRNAQ), Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre, Université de Bouira, Bouira 10000, Algeria
6
Département des Sciences Biologiques, Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre, Université de Bouira, Bouira 10000, Algeria
7
Laboratoire de Génie des Procédés Chimiques, Département de Génie des Procédés, Faculté de Technologie, Université Ferhat Abbas, Sétif-1, Sétif 19000, Algeria
8
Ecole Nationale Supérieure de Chimie de Rennes, University of Rennes, CNRS, ISCR—UMR6226, 35000 Rennes, France
9
Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
10
Laboratory of Reaction Engineering, USTHB, BP 32, Algiers 16111, Algeria
11
School of Engineering, Merz Court, Newcastle University, Newcastle upon Tyne NE1 7RU, UK
 
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*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(1), 370; https://doi.org/10.3390/ijms26010370
Submission received: 7 November 2024 / Revised: 11 December 2024 / Accepted: 22 December 2024 / Published: 4 January 2025
(This article belongs to the Special Issue Molecular Studies of Natural Compounds and Plant Extracts—2nd Edition)
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Abstract

This study examines the chemical composition, antioxidant properties, and urease inhibitory effects of Hyoscyamus muticus L. subsp. falezlez (Coss.) Maire. Using LC-ESI-MS/MS, 19 distinct phenolic compounds were identified, with chlorogenic acid being the most abundant. The ethanol extract demonstrated notable antioxidant activity, highlighting its potential for therapeutic use. Urease inhibition assays revealed a remarkable 91.35% inhibition by the H. muticus extract, with an IC50 value of 5.6 ± 1.20 μg/mL, indicating its promising role in addressing conditions linked to urease activity. Molecular docking studies further investigated the interaction between H. muticus phenolic compounds and urease, identifying hyperoside as a leading candidate, with a binding energy of −7.9 kcal/mol. Other compounds, such as rutin, luteolin, apigenin, kaempferol, hesperetin, chlorogenic acid, and rosmarinic acid, also demonstrated significant binding affinities, suggesting their potential to disrupt urease function. These findings highlight the therapeutic potential of H. muticus as a source of natural bioactive compounds, offering promising avenues for the development of novel treatments for urease-related disorders and oxidative stress.
Keywords: Hyoscyamus muticus; LC-ESI-MS/MS; phenolic compounds; antioxidant activity; urease inhibition; molecular docking Hyoscyamus muticus; LC-ESI-MS/MS; phenolic compounds; antioxidant activity; urease inhibition; molecular docking

Share and Cite

MDPI and ACS Style

Lekmine, S.; Benslama, O.; Bensalah, B.; Touzout, N.; Moussa, H.; Tahraoui, H.; Ola, M.S.; Hafsa, H.; Zhang, J.; Amrane, A. Bioactive Phenolics of Hyoscyamus muticus L. Subsp. Falezlez: A Molecular and Biochemical Approach to Antioxidant and Urease Inhibitory Activities. Int. J. Mol. Sci. 2025, 26, 370. https://doi.org/10.3390/ijms26010370

AMA Style

Lekmine S, Benslama O, Bensalah B, Touzout N, Moussa H, Tahraoui H, Ola MS, Hafsa H, Zhang J, Amrane A. Bioactive Phenolics of Hyoscyamus muticus L. Subsp. Falezlez: A Molecular and Biochemical Approach to Antioxidant and Urease Inhibitory Activities. International Journal of Molecular Sciences. 2025; 26(1):370. https://doi.org/10.3390/ijms26010370

Chicago/Turabian Style

Lekmine, Sabrina, Ouided Benslama, Bachir Bensalah, Nabil Touzout, Hamza Moussa, Hichem Tahraoui, Mohammad Shamsul Ola, Haroun Hafsa, Jie Zhang, and Abdeltif Amrane. 2025. "Bioactive Phenolics of Hyoscyamus muticus L. Subsp. Falezlez: A Molecular and Biochemical Approach to Antioxidant and Urease Inhibitory Activities" International Journal of Molecular Sciences 26, no. 1: 370. https://doi.org/10.3390/ijms26010370

APA Style

Lekmine, S., Benslama, O., Bensalah, B., Touzout, N., Moussa, H., Tahraoui, H., Ola, M. S., Hafsa, H., Zhang, J., & Amrane, A. (2025). Bioactive Phenolics of Hyoscyamus muticus L. Subsp. Falezlez: A Molecular and Biochemical Approach to Antioxidant and Urease Inhibitory Activities. International Journal of Molecular Sciences, 26(1), 370. https://doi.org/10.3390/ijms26010370

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