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Article

Characterization of Leptin and Leptin Receptor Gene in the Siberian Sturgeon (Acipenser baerii): Molecular Cloning, Tissue Distribution, and Its Involvement in Feeding Regulation

by
Hongwei Wu
1,†,
Jiamei Li
1,†,
Kezhen Jiang
1,
Yingzi Li
1,
Zhaoxiong Yu
2,
Bin Wang
2,
Bo Zhou
2,
Xin Zhang
1,
Ni Tang
1,2,* and
Zhiqiong Li
1,*
1
Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, No. 211 Huimin Road, Chengdu 611130, China
2
Fisheries Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611731, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2025, 26(5), 1968; https://doi.org/10.3390/ijms26051968
Submission received: 11 December 2024 / Revised: 17 February 2025 / Accepted: 21 February 2025 / Published: 25 February 2025
(This article belongs to the Special Issue Fish Nutrition, Metabolism and Physiology)
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Abstract

Leptin is an adipokine known as a regulator of feeding and metabolism in mammals. Previous studies on fish have revealed its role in food intake regulation in limited teleosts. However, its specific function in Siberian sturgeon, an ancient Chondrostei fish, remains poorly understood. This study represents the first successful cloning of sequences for leptin and leptin receptors in Siberian sturgeon, achieved using RT-PCR. The predicted leptin sequence in this species consists of 168 amino acids that exhibit low identity with other fish species, except within the Acipenseriformes order. Tissue distribution analysis revealed a high expression of Siberian sturgeon leptin mRNA in the liver and lepr mRNA in the hypothalamus. Fasting differentially affected the expression of leptin and lepr mRNA, with decreased levels in the hypothalamus and increased levels in the liver (leptin: 3–15 days; lepr: 6–15 days). Recombinant Siberian sturgeon leptin (Ssleptin) was produced via E. coli expression, and intraperitoneal injection (100 ng/g BW) significantly inhibited food intake. The anorectic effect was correlated with changes in hypothalamic gene expression, including downregulation of orexigenic factors (agrp, orexin, npy, and ghrelin) and upregulation of anorexigenic factors (pomc, mch, and insulin). Meanwhile, the peripheral administration of Ssleptin promoted the expression of resistin in the liver and concurrently increased cck and pyy mRNA levels in the valvular intestine. Furthermore, Ssleptin injection stimulated the expression of hypothalamic lepr, jak2, akt, and ampkα2 mRNA. These findings suggest that leptin plays a significant role in the feeding control of Siberian sturgeon and provide new insights into the evolutionary function of leptin in fish.
Keywords: food intake; leptin; appetite regulation; appetite; fasting food intake; leptin; appetite regulation; appetite; fasting

Share and Cite

MDPI and ACS Style

Wu, H.; Li, J.; Jiang, K.; Li, Y.; Yu, Z.; Wang, B.; Zhou, B.; Zhang, X.; Tang, N.; Li, Z. Characterization of Leptin and Leptin Receptor Gene in the Siberian Sturgeon (Acipenser baerii): Molecular Cloning, Tissue Distribution, and Its Involvement in Feeding Regulation. Int. J. Mol. Sci. 2025, 26, 1968. https://doi.org/10.3390/ijms26051968

AMA Style

Wu H, Li J, Jiang K, Li Y, Yu Z, Wang B, Zhou B, Zhang X, Tang N, Li Z. Characterization of Leptin and Leptin Receptor Gene in the Siberian Sturgeon (Acipenser baerii): Molecular Cloning, Tissue Distribution, and Its Involvement in Feeding Regulation. International Journal of Molecular Sciences. 2025; 26(5):1968. https://doi.org/10.3390/ijms26051968

Chicago/Turabian Style

Wu, Hongwei, Jiamei Li, Kezhen Jiang, Yingzi Li, Zhaoxiong Yu, Bin Wang, Bo Zhou, Xin Zhang, Ni Tang, and Zhiqiong Li. 2025. "Characterization of Leptin and Leptin Receptor Gene in the Siberian Sturgeon (Acipenser baerii): Molecular Cloning, Tissue Distribution, and Its Involvement in Feeding Regulation" International Journal of Molecular Sciences 26, no. 5: 1968. https://doi.org/10.3390/ijms26051968

APA Style

Wu, H., Li, J., Jiang, K., Li, Y., Yu, Z., Wang, B., Zhou, B., Zhang, X., Tang, N., & Li, Z. (2025). Characterization of Leptin and Leptin Receptor Gene in the Siberian Sturgeon (Acipenser baerii): Molecular Cloning, Tissue Distribution, and Its Involvement in Feeding Regulation. International Journal of Molecular Sciences, 26(5), 1968. https://doi.org/10.3390/ijms26051968

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