Irradiation Effect on Oxidative Condition and Tocopherol Content of Vegetable Oils
1
Department of Food Technology, Technological Educational Institution (T.E.I.) of Larissa, Karditsa, GR-43100, Greece
2
Department of Food Technology, Technological Educational Institution (T.E.I.) of Athens, Egaleo, Athens, GR-12210, Greece
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2007, 8(6), 533-540; https://doi.org/10.3390/i8060533
Submission received: 30 April 2007
/
Accepted: 22 May 2007
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Published: 15 June 2007
Abstract
The effect on induction period and tocopherol content after γ-irradiation on samples of olive oil and seed oils (sunflower and soybean) was determined. In seed oil samples 0, 100, 200 and 300 ppm of δ-tocopherol were added before irradiation with 1, 2 and 3kGy. The results of induction period showed that, after irradiation, all samples presented a significant decreased in resistance to oxidation. However, this decrease was minimized when δ-tocopherol was added. Irradiation significantly decreased the level of tocopherols. δ-Tocopherol appeared more sensitive in irradiation process than α- and γ-tocopherol. The addition of δ-tocopherol significantly reduced, in most cases, the depletion of the other tocopherols.
1. Introduction
Ionizing radiations are used for the treatment of various food products. However, only electromagnetic waves (visible light, x-rays, γ-rays, ultraviolet light, infrared light, etc) and electrons can be used in food process, while neutrons, deuterons and α-rays cause extensive damage to food [1]. The irradiation extends the storage life of food and improves the safety via the reduction of pathogenic and spoilage micro-organisms [2]. The ionizing radiation has the ability to start a high number of chemical changes in gas, liquid and solid phase of food. With the exposure in irradiation initially ionization of water takes place followed by the production of free radicals and peroxides [3]. Many intermediate products are formed and react each other or with other components of the system [1]. The reactions of these intermediate products with the ingredients of food are numerous. Each group of food ingredients such as carbohydrates, proteins and other nitrogen containing compounds, fats and oils, vitamins, enzymes and coloring compounds can react with certain intermediate products and form new intermediate products, many of which have high chemical activity [3–5]. The reactions of oxidation, free radical forming and reduction belong to this category.
Stevenson [6] proved that irradiation does not have repercussions in the general nutritional value of foods and the oxidative changes caused are similar to those observed with the use of conventional techniques of food treatment. Nevertheless, a combination of irradiation and other methods of treatment, such as cooking, can lead to accelerating oxidative destruction of foods [7]. The storage of irradiated lipid food, in the presence of oxygen, accelerates the autoxidation [8]. It has been proved that irradiation accelerates the oxidation of unsaturated fatty acids and cholesterol during frozen storage of foods [9–10]. In lipids, many of the reactions resemble to those of the peroxidation from molecular oxygen, but special reactions can also take place owed exclusively to ionizing radiation [1].
Vitamin E is the most labile in irradiation from all fat soluble vitamins [11–13]. Irradiation decreased α-tocopherol levels during irradiation of various kinds of meat [11–13]. The addition of tocopherols (in animal feed) leaded to the protection of phospholipids and cholesterol by breaking the chain of oxidation in meat fat [14]. Moreover, it prevented the loss of water detention, color and flavor of food during storage.
As determined by Fuster et al. [15], triacylglycerols can be used as a model system (which provides the natural complexity of fatty distribution). So, a vegetable oil can be used to determine the effect of irradiation on lipids without interference of other food ingredients (proteins, carbohydrates, etc.).
In this work, we tried to determine the effect of γ-irradiation on oxidative condition and tocopherol content of lipids using various vegetable oils as model systems. The oxidative condition before and after irradiation of seed oil samples with addition of δ-tocopherol was also determined. δ-Tocopherol was selected since, to our knowledge, there were no previous reports on the effect of irradiation on that tocopherol. Additionally, δ-tocopherol was not expected [16, 17] to show antioxidant action higher than its α-homolog during irradiation since the process was carried out at room temperature.
2. Results and Discussion
The results of induction period (Table 1) showed that irradiation caused a significant (P < 0.05) decrease in resistance to oxidation of all samples. As expected, olive oil samples presented a longer period of induction. After irradiation with 3 KGy, the lowest decrease was observed in olive oil samples (9.6 %) and the highest in soybean oil (34.2 %). The oxidative stability of olive oil is related to some extent to the presence of α-tocopherol [18]. Kiritsakis and Min [19] reported that olive oil contains up to 150 mg/kg α-tocopherol. However, the stability of olive oil could not be explained only on the basis of tocopherol action. It is known that the olive mesocarp contains phenolic compounds, which are present in the olive oil and considerably increase the stability against oxidation of the oil [19]. To these compounds, α-tocopherol and of course the fatty acid composition [18] should be attributed the lowest decrease (Table 2) of α-tocopherol in olive oil after irradiation (68.3 %) in contrast to the other two oils. These results agree with those of Zhu et al. [20] who reported that depletion of α-tocopherol (during protection of low density lipoproteins against oxidation) was delayed after addition of certain flavonoids which regenerated the vitamin.
In all cases, the addition of δ-tocopherol significantly (P < 0.05) increased the induction period (at the same irradiation dose) of soybean and sunflower oil (Table 1). Particularly, the increase of induction period was up to 28.9 % and 34.8 % at 3 KGy for sunflower and soybean oil, respectively. Additionally, the reduction of induction period, during the progress of the irradiation, appeared proportional to the level of addition of δ-tocopherol. Specifically, the reduction of induction period after 3 KGy dose of irradiation of soybean oil was from 34.2 % (control) to 21.4 % (300 ppm of δ-tocopherol) and sunflower oil from 25.8 % (control) to 18.0 % (300 ppm of δ-tocopherol). These results are in line with those reported by Bourgeois and Czornomaz [16] and Von Pongracz et al. [17] who reported that the antioxidant activity of tocopherols is δ>γ>α>β in a temperature between 80 and 120 °C and α > γ > β > δ in a temperature between 20 and 60 °C. During the determination of the induction period (Rancimat method) where the temperature was set at 90 °C, δ-tocopherol increased the induction period by acting as antioxidant with higher activity.
As shown in Table 2, irradiation decreased the level of tocopherols. This decrease was significant (P < 0.05) in all samples. The highest decrease in α-tocopherol was shown in soybean oil without additional δ-tocopherol (up to 92.6 %). δ-Tocopherol showed even higher decrease (up to 93.1 %) and appeared more sensitive in irradiation process than α-tocopherol, while γ-tocopherol appeared the most resistant (up to 92.3 % of reduction). However, it should be stated that in samples with low tocopherol concentration the depletion was complete (Table 2). Lakritz and Thayer [11] who reported a decrease in α- and γ-tocopherol (30 and 15 %, respectively) after irradiation of fresh chicken breasts. Additionally, Lakritz and Thayer [12] reported a decrease (6 %) in α-tocopherol but no significant changes in γ-tocopherol after irradiation. In the study of Lakritz et al. [13], α-tocopherol decreased significantly in meat irradiated with 0–9.4 kGy at 5 °C. On the contrary, Galvin et al. [7], who studied the retention of α-tocopherol and lipid oxidation after irradiation (2.5 and 4.0 kGy) in cooked minced chicken (fed with a diet supplemented with α-tocopheryl acetate), reported no affect of irradiation on the α-tocopherol content. Concerning the lipid stability, the same authors indicated that oxidation (measured by thiobarbituric acid-reacting substances and cholesterol oxidation products) was accelerated during storage but was possible to control it when chicken diet was supplemented with of α-tocopheryl acetate. It appears that there is a disagreement between various authors on the percentage of tocopherol depletion as a consequence of irradiation which can be attributed to the different experimental conditions and samples. The results of our work are also difficult to be compared with the aforementioned studies since it was conducted under different experimental conditions (room temperature) and on different samples (vegetable oils).
The mechanism of the antioxidant action of tocopherols is thoroughly described by many authors [21–22]. Extensive studies have also been reported on the synergistic behavior of tocopherols and other vitamins [2, 15, 23–25] and flavonoids [20, 26]. Niki and Noguchi [27] reported that α-tocopherol acts as an antioxidant not only by itself but also in collaboration with other antioxidants. The addition of 100, 200 or 300 ppm δ-tocopherol in seed oil samples, and hence the significant increase of the coantioxidant (δ-tocopherol) concentration, reduced the depletion of all tocopherols (Table 2) after irradiation with 1, 2 and 3KGy. α-Tocopherol depletion was reduced by 15.4 % and 18.5 % (for soybean and sunflower oil, respectively), γ-tocopherol by 19.2 % (for soybean oil) and δ-tocopherol by 5.2 % and 12.8 % (for soybean and sunflower oil, respectively). These results can explain why the drop of induction period of all oils was reduced when δ-tocopherol was added. The results are in agreement with those of Zhu et al. [20] who demonstrated that LDL oxidation did not occur substantially until a-tocopherol was depleted.
In conclusion, the present study demonstrated that the effect of irradiation on the oxidative condition of oils was minimized when δ-tocopherol was added. At the same time the tocopherol content was reduced considerably. The addition of δ-tocopherol can reduce, in most cases, the loss of α- and γ-tocopherols and hence retain the nutritional value of lipid foods. From a nutritional perspective, the protection of α-tocopherol, which possesses over 80 % of biological activity of all tocopherols, is important since this vitamin is an integral component of biological membranes, where it acts as a highly effective chain-breaking antioxidant and inhibits phospholipids and cholesterol oxidation [14]. Further investigation on the effect of irradiation on tocopherol content and induction period of lipid foods using higher doses and δ-tocopherol concentrations should be carried out in combination to the mechanism of action of various tocopherols.
3. Experimental Section
Three different vegetable oils were used, namely olive oil, sunflower oil and soybean oil (all purchased from Elais s.a, Athens, Greece). In sunflower and soybean oil samples 0, 100, 200 or 300 ppm of δ-tocopherol (Sigma Chemicals Company Ltd., St. Louis, USA) were added (Table 2) before irradiation takes place. Each determination was also carried out on a sample of seed oil (Control) (with or without addition of δ-tocopherol) not subjected to irradiation.
3.1 Irradiation
Oils were placed in sealed screw capped glass vials (5 cm in length, i.d. 2 cm) and then irradiated with doses of 1, 2, and 3 kGy, at room temperature, using as gamma source 60Co (Theratron 1000 for radiotherapy applications, MDS Nordion, Ottawa, Canada). The irradiation was carried out at Department of Oncology and Radiotherapy of I.K.A. in G. Genimatas Oncology Hospital (Athens, Greece). The irradiated samples and unirradiated controls were stored, with nitrogen induced, at −20 °C for approximately 1 day before induction period and tocopherol content determination.
3.2 Rancimat method
The determination was carried out using the method described by Lalas and Dourtoglou [28]. Two and a half grams of oil were accurately weighed into the reaction vessel of the Rancimat 679 (Metrhom LTD, Herisau, Switzerland). The conditions were set at 90 °C and 15 liter/h.
3.3 Tocopherols
The determination was carried out using the method described by Lalas and Tsaknis [29]. An HPLC pump, Waters 600E (Millipore Corporation, Waters Chromatography Division, Massachusetts, USA), equipped with a Waters μ-Porasil column (125 Å, 10 μm, 3.9x300 mm) and a Waters 486 Tunable Absorbance Detector was used.
3.4 Statistical analysis
Results are displayed as means of triplicate determinations and standard deviation (in parenthesis). Statistical significance of the differences between mean values was assessed by ANOVA test.
| Oil | Added δ-tocopherol (ppm) | Control | Irradiated 1KGy | Irradiated 2KGy | Irradiated 3KGy |
|---|---|---|---|---|---|
| Olive | 0 | 149.7 a* (1.5) | 143.2b (0.3) | 139.8 c (1.9) | 135.3 d (0.4) |
| Soybean | 0 | 31.0 aA (0.2) | 29.9 bA (0.3) | 28.9 cA (0.2) | 20.4 dA (0.2) |
| 100 | 31.7 aB (0.6) | 30.9 bB (0.3) | 30.0 cB (0.4) | 23.2 dB (0.3) | |
| 200 | 32.9 aC (0.2) | 32.4 bC (0.2) | 31.7 cC (0.2) | 25.1 dC (0.2) | |
| 300 | 35.0 aD (0.2) | 33.2 bD (0.2) | 32.8 cD (0.3) | 27.5 dD (0.4) | |
| Sunflower | 0 | 23.3 aA (0.6) | 22.0 bA (0.2) | 21.3 cA (0.2) | 17.3 dA (0.3) |
| 100 | 25.0 aB (0.5) | 22.9 bB (0.2) | 21.9 cB (0.4) | 19.0 dB (0.3) | |
| 200 | 26.3 aC (0.6) | 24.0 bC (0.2) | 23.3 cC (0.2) | 20.2 dC (0.3) | |
| 300 | 27.2 aD (0.3) | 25.8 bD (0.2) | 24.8 cD (0.3) | 22.3 dD (0.4) | |
*Values are mean and standard deviation (in parenthesis) of triplicate determinations. Means of each individual tocopherol within each row with different superscripts are significantly (P < 0.05) different. Means of each individual tocopherol for each oil within each column with different subscripts are significantly (P < 0.05) different.
| Oil | Added δ-tocopherol (ppm) | α-tocopherol | γ-tocopherol | δ-tocopherol | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | 1KGy | 2KGy | 3KGy | Control | 1KGy | 2KGy | 3KGy | Control | 1KGy | 2KGy | 3KGy | ||
| Olive | 0 | 151.7a (11.5) | 100.2 b (4.8) | 82.1 c (0.5) | 48.1 d (0.4) | 9.9 a (1.2) | 3.1b (0.1) | 0.0 c (0.0) | 0.0c (0.0) | 4.6 a (0.4) | 1.0 b (0.1) | 0.0 c (0.0) | 0.0c (0.0) |
| Soybean | 0 | 127.0aA (2.0) | 71.7 bA (1.5) | 32.3cA (1.9) | 9.4 dA (0.2) | 512.3 aA (18.2) | 237.6 bA (2.2) | 108.5 cA (1.5) | 39.7 dA (1.5) | 293.0aA (10.8) | 117.2 bA (1.7) | 57.3cA (6.5) | 20.2 dA (0.6) |
| 100 | 129.0aA (1.0) | 82.3 bB (0.4) | 41.2 cB (0.7) | 13.7 dB (0.4) | 516.3 aA (1.2) | 289.2 bB (2.5) | 130.0 cB (1.7) | 68.4 dB (1.2) | 390.7aB (2.1) | 156.3 bB (1.5) | 90.7cB (1.5) | 39.2 dB (1.1) | |
| 200 | 128.3aA (1.5) | 98.9 bC (0.4) | 53.6 cC (2.4) | 20.1 dC (0.3) | 511.7 aA (3.8) | 331.3 bC (1.1) | 164.7 cC (1.5) | 101.2 dC (1.1) | 470.0aC (18.0) | 197.4 bC (2.1) | 117.3 cC (7.5) | 42.3 dC (1.1) | |
| 300 | 130.7aA (2.1) | 102.7 bD (0.8) | 67.0 cD (1.0) | 29.8 dD (1.0) | 511.0 aA (7.0) | 399.7 bD (1.5) | 194.3 cD (3.8) | 137.6 dD (1.1) | 601.3aD (1.1) | 216.2 bD (1.1) | 202.3 cD (2.5) | 72.3 dD (0.6) | |
| Sunflower | 0 | 530.3aA (8.1) | 287.3 bA (2.0) | 193.5 cA (5.6) | 62.1 dA (0.9) | 12.0aA (0.1) | 3.6bA (0.3) | 0.9 cA (0.2) | 0.0dA (0.0) | 8.8 aA (0.7) | 2.1 bA (0.2) | 0.0 cA (0.0) | 0.0dA (0.0) |
| 100 | 527.7 aA (3.2) | 340.1 bB (4.3) | 221.0 cB (3.0) | 95.2 dB (0.7) | 11.7 aA (0.3) | 5.1bB (0.4) | 1.2 cB (0.1) | 0.0 dA (0.0) | 106.0 aB (1.7) | 41.5 bB (1.3) | 20.7 cB (0.6) | 8.5 dB (0.5) | |
| 200 | 534.0aA (7.8) | 399.2 bC (3.7) | 263.0 cC (6.1) | 137.1 dC (1.2) | 11.9aA (0.2) | 7.4bC (0.4) | 1.5 cC (0.1) | 0.0dA (0.0) | 206.0aC (5.3) | 76.2bC (1.1) | 43.7cC (3.5) | 24.4 dC (1.9) | |
| 300 | 537.3aA (2.5) | 448.8bD (3.4) | 299.7 cD (0.7) | 162.4 dD (1.2) | 12.0aA (0.2) | 9.3bD (0.3) | 1.7 cD (0.1) | 0.0dA (0.0) | 304.3aD (4.9) | 116.0 bD (2.0) | 63.7cD (3.5) | 39.1 dD (1.9) | |
Values are mean and standard deviation (in parenthesis) of triplicate determinations. Means of each individual tocopherol within each row with different superscripts are significantly (P < 0.05) different. Means of each individual tocopherol for each oil within each column with different subscripts are significantly (P < 0.05) different.
Acknowledgements
The study has been co-funded by 75 % from European Union and 25 % from the Greek Government under the framework of the Education and Initial Vocational Training Program – EPEAEK I Archimedes.
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Lalas, S.; Gortzi, O.; Tsaknis, J.; Sflomos, K. Irradiation Effect on Oxidative Condition and Tocopherol Content of Vegetable Oils. Int. J. Mol. Sci. 2007, 8, 533-540. https://doi.org/10.3390/i8060533
AMA Style
Lalas S, Gortzi O, Tsaknis J, Sflomos K. Irradiation Effect on Oxidative Condition and Tocopherol Content of Vegetable Oils. International Journal of Molecular Sciences. 2007; 8(6):533-540. https://doi.org/10.3390/i8060533
Chicago/Turabian StyleLalas, Stavros, Olga Gortzi, John Tsaknis, and Konstantinos Sflomos. 2007. "Irradiation Effect on Oxidative Condition and Tocopherol Content of Vegetable Oils" International Journal of Molecular Sciences 8, no. 6: 533-540. https://doi.org/10.3390/i8060533
APA StyleLalas, S., Gortzi, O., Tsaknis, J., & Sflomos, K. (2007). Irradiation Effect on Oxidative Condition and Tocopherol Content of Vegetable Oils. International Journal of Molecular Sciences, 8(6), 533-540. https://doi.org/10.3390/i8060533
