Next Article in Journal
Methyl 6,7-Difluoro-2-[(4-fluorobenzyl)sulfanyl]-4-hydroxyquinoline-3-carboxylate
Previous Article in Journal
Ac-[K-Aib-C(3,9-Acm; 6,12-DFLC(Acm)KKESEK)]4-NH2
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Short Note

(R)-2-Amino-1-hydroxyethylphosphonic Acid

Department of Chemistry, Joseph Priestley Building, Queen Mary University of London, Mile End Road, London E1 4NS, UK
*
Author to whom correspondence should be addressed.
Molbank 2024, 2024(4), M1888; https://doi.org/10.3390/M1888
Submission received: 7 September 2024 / Revised: 22 September 2024 / Accepted: 24 September 2024 / Published: 26 September 2024

Abstract

:
(R)-2-Amino-1-hydroxyethylphosphonic acid 2 was prepared by hydrolytic kinetic resolution of rac-diethyl oxiran-2-ylphosphonate followed by reaction with benzylamine, acid hydrolysis, catalytic hydrogenolysis, and anion-exchange chromatography. Recrystallization from water-ethanol gave pure 2, which was characterized by IR, 1H NMR, 13C NMR, 31P NMR, polarimetry, elemental microanalysis, high-resolution mass spectrometry, and single-crystal X-ray diffraction. The acid 2 crystallized in the orthorhombic noncentrosymmetric space group P212121 with cell parameters a = 6.303 (2) Å, b = 7.104 (2) Å, c = 11.627 (3) Å. The X-ray crystal structure confirmed the (R)-configuration of 2 and revealed that 2 is zwitterionic in the solid state, with extensive intermolecular hydrogen bonding between the hydroxyl, ammonium cation, and phosphonate anion groups.

Graphical Abstract

1. Introduction

Phosphorus is essential for all life on Earth, but the low bioavailability of this vital element in many marine environments places stress on biological systems [1]. Microorganisms can use phosphonic acid derivatives to manage scarce phosphorus resources. Thus, in phytoplankton, 2-aminoethylphosphonic acid 1 (Scheme 1) can act as a reservoir for supplying inorganic phosphate 3. The oxidation of 2-aminoethylphosphonic acid 1, mediated by the iron-dependent enzyme PhnY, leads to the stereoselective formation of (R)-2-amino-1-hydroxyethylphosphonic acid 2. This catabolic intermediate 2 is the precursor to inorganic phosphate 3, which is released alongside glycine 4 following further stereoselective oxidation, mediated by a second iron-dependent enzyme, PhnZ [2]. A 2.1 Å resolution X-ray crystal structure of the PhnZ enzyme bound to its substrate 2 has been reported [3].
Much elegant work has been done to develop methods for the stereoselective synthesis of organophosphorus compounds, and this has been the subject of a recent review article [4]. Previously, we have shown [5] that enantioselective chemical synthesis of the natural product 2 can be achieved using hydrolytic kinetic resolution of racemic epoxyphosphonate 5 in the presence of the Jacobsen [6] cobalt catalyst 6 (Scheme 2). The unchanged (R)-enantiomer of the epoxide 5 was ring-opened by benzylamine to give the intermediate 7, then all protecting groups were removed to afford (R)-2, with physical data consistent with those of material obtained from natural sources [7], by preparation of diastereoisomeric salts [7] and by kinetic resolution of an ester precursor using a lipase [8]. Recently, the formation of 2 by borane reduction of a nitrile precursor was used to determine the absolute configuration of the natural product (–)-hydroxynitralaphos [9].
There have been only a few crystal structures reported for aminophosphonic acids. These include compounds 1 [10] and 811 (Figure 1) [11,12,13,14]. In this article, we provide experimental details and characterization for the chemical synthesis of the acid 2, and we report a high resolution (0.7 Å) single crystal X-ray structure determination of 2, thus defining the hydrogen bond network that exists in the solid state for pure (R)-2, a small but highly functionalized chiral molecule.

2. Results

The synthesis of 2 proceeded as previously outlined [5]; product 2 was recrystallized from aqueous ethanol, then dried under vacuum. Physical data for 2, including NMR spectra (Figures S1–S4), melting point, and optical rotation, matched existing literature. An FT-IR spectrum was recorded (Figure S5) and was consistent with the presence of O–H and N–H groups. High-resolution mass spectrometry (Figure S6) confirmed the molecular formula, and elemental analysis demonstrated that the crystals did not contain residual solvent.
Single-crystal X-ray diffraction (Figure 2) revealed the structure of 2, which exists in the noncentrosymmetric space group P212121; a CIF report for 2 is included in the Supplementary Materials. The presence of the relatively heavy phosphorus atom within the compound allowed the absolute configuration to be confirmed with confidence: a Flack [15] absolute structure parameter of 0.023 (13) was calculated for the (R)-enantiomer. Molecules of 2 exist as zwitterions with transfer of one proton from each phosphonic acid group to the amino group generating phosphonate anions and ammonium cations. In contrast, the crystal structure for 2 that is bound to the PhnZ enzyme [3] shows complete deprotonation of the phosphonic acid group, which is coordinated through oxygen to one of the iron atoms in the active site of the enzyme. The conformation of each molecule in the structure of pure 2 is such that the phosphorus and nitrogen atoms are antiperiplanar to one another, with P–C(1)-C(2)-N torsion angles of 173.8°, similar to the value of 174.2° found for 2 when it is bound to the PhnZ enzyme [3] and to the conformations of compounds 1 and 8 observed in previous reports [10,11]. The polar functional groups of 2 engage in complex networks of hydrogen bonds between neighboring molecules of 2; no intramolecular hydrogen bonds are observed and, in accord with the microanalysis results, no solvent molecules are retained within the crystals. This absence of solvation contrasts with compounds rac-10b, which crystallized as a hydrate, and rac-11, which contained ethanol of crystallization.
For each molecule of acid 2 in the crystal, there were four N–H•••O and two O–H•••O type intermolecular hydrogen bonds to adjacent molecules of 2 (Table 1). Figure 3 illustrates the packing of molecules of 2 within the crystal and the associated network of hydrogen bonds. The ammonium and phosphonate groups adopt similar hydrogen bond-forming roles to those seen in crystals of compounds 1 and 811. All three N–H groups are hydrogen-bond donors, with bifurcation at H4. The three oxygen atoms of the phosphonate group are all hydrogen-bond acceptors, with O2 and O3 each linking to two H atoms in neighboring molecules. Additionally, the phosphonate O1–H7 and the alcohol O4–H8 groups act as both hydrogen-bond donors and acceptors.
To conclude, we have established the crystal structure of enantiomerically pure 2, prepared according to our previously outlined method, for which experimental details are now provided. Notably, this highly polar substance crystallizes from aqueous ethanol without retaining solvent. In common with other aminophosphonic acids, 2 is zwitterionic. All the functional groups of 2 contribute to intermolecular hydrogen bonding within the crystal.

3. Experimental

3.1. General Experimental Details

Unless otherwise stated, all commercially available solvents and reagents were used without further purification. Melting points were measured using a hot-stage microscope (Reichert, Vienna, Austria). FT-IR spectra were obtained using KBr disks with an 8300 spectrometer (Shimadzu, Kyoto, Japan). NMR spectra were recorded using AM250 (Bruker UK Ltd., Coventry, UK), AVIII 400 (Bruker UK Ltd., Coventry, UK) and JNM-EX270 (Jeol, Tokyo, Japan) spectrometers. Elemental microanalysis was performed by Medac Ltd., Chobham, Surrey, UK. Mass spectra were obtained by the Engineering and Physical Sciences Research Council National Mass Spectrometry Facility (EPSRC NMSF), Swansea, using an LTQ Orbitrap XL instrument (Thermo Scientific, Waltham, MA, USA) with an electrospray ion source and electron multiplier detector.

3.2. (R)-Diethyl Oxiran-2-ylphosphonate (R)-5

AcOH (7 μL, 0.12 mmol) was added to a solution of (R,R)-(–)-N,N′-bis(3,5-di-tert-butylsalicylidene)-1,2-cyclohexanediaminocobalt(II) 6 (38.0 mg, 0.06 mmol) in dry toluene (1.5 mL) and stirred in air at 20 °C for 1 h. The acetic acid and toluene were evaporated under vacuum and rac-diethyl oxiran-2-ylphosphonate 5 [16] (1.261 g, 7.00 mmol) was added, followed by water (101 μL, 5.60 mmol). Stirring was continued for 4 d, after which purification by flash chromatography (CH2Cl2-EtOAc, 3:1) gave (R)-diethyl oxiran-2-ylphosphonate (R)-5 (520.1 mg, 2.71 mmol) [ee > 95%, based on 31P NMR integration of the product of epoxide opening with neat (S)-α-methylbenzylamine (5 equivalents, 16 h, 20 °C)] [5].

3.3. (R)-Diethyl (2-benzylamino-1-hydroxyethyl)phosphonate 7

A mixture of (R)-diethyl oxiran-2-ylphosphonate 5 (455.3 mg, 2.53 mmol) and benzylamine (1.35 g, 12.6 mmol) was kept at 20 °C for 16 h, after which the excess benzylamine was evaporated in vacuo (70 °C, 0.5 mmHg, 1 h). The residue was subjected to flash chromatography (gradient from EtOAc-CH2Cl2 1:1 to EtOH-EtOAc 3:7). Appropriate fractions were pooled and then evaporated; subsequently, the residue was taken up in Et2O, filtered through Celite to remove silica gel residues, and re-evaporated to give (R)-diethyl (2-benzylamino-1-hydroxyethyl)phosphonate 7 (557.5 mg, 77%) as a liquid [α]D30 −7.6 (c = 1.67, MeOH); δH (270 MHz, CDCl3) 7.37–7.20 (5H, m), 4.22–4.09 (4H, m), 4.00 (1H, dd, J = 11.2, 6.8 Hz), 3.80 (2H, s), 3.38 (2H, br s), 3.03–2.86 (2H, m), 1.35–1.28 (6H, m).

3.4. (R)-2-Amino-1-hydroxyethylphosphonic Acid 2

(R)-Diethyl (2-benzylamino-1-hydroxyethyl)phosphonate 7 (272.4 mg, 0.95 mmol) was refluxed gently for 16 h with 12 M hydrochloric acid (20 mL) and then evaporated in vacuo. Water (16 mL), acetic acid (8 mL), and palladium black (120 mg) were added, and the mixture was stirred under a balloon of hydrogen at room temperature for 48 h. The mixture was filtered through Celite, and the solvent was evaporated in vacuo. The residue was re-evaporated from water, then redissolved in water and applied to a column of Amberlite IR400 (OH), which was eluted first with water and then with 10% aqueous AcOH. The ninhydrin-positive fractions were combined and evaporated. The residue was recrystallized from water-ethanol to give the title compound 2 (109.3 mg, 82%) as colorless needles, mp 264–265 °C (lit. [7] 244–246 °C); [α]D27 −31.9 (c = 0.525, H2O) (lit. [7] [α]D20 −30.5). Found: C, 17.10; H, 5.73; N, 9.57 calcd for C2H8NO4P: C, 17.03; H, 5.72; N, 9.92; νmax/cm−1 (KBr) 3458, 3157, 2936, 2640, 1663, 1539, 1259, 1159, 1096, 937, 781; δH (400 MHz, D2O) 3.90 (1H, td, J = 9.9, 2.7 Hz), 3.30 (1H, ddd, J = 13.2, 6.4, 3.2 Hz), 3.11 (1H, ddd, J = 13.3, 9.8, 6.2 Hz); δc (62.9 MHz, D2O) 62.7 (d, 1JCP = 155 Hz), 44.4 (d, 2JCP = 8 Hz); δP (162 MHz, D2O) 15.1; m/z (ESI) found: 142.0262; calcd for C2H9NO4P (M+H+): 142.0264.

3.5. X-ray Structure Determination of 2

Single-crystal X-ray diffraction was carried out at the Queen Mary University of London using the KAPPA APEX ii DUO diffractometer (Bruker UK Ltd., Coventry, UK), with MoKα radiation (λ = 0.71073 Å). X-ray crystal structures were solved and refined using the Bruker SHELXTL version 2018/2 software package.
A translucent colorless block-like specimen of C2H8NO4P, approximate dimensions 0.100 mm × 0.120 mm × 0.200 mm, was used for the X-ray crystallographic analysis. The X-ray intensity data were measured. A total of 2253 frames were collected. The total exposure time was 6.26 h. The frames were integrated with the Bruker SAINT V8.18C software package using a narrow-frame algorithm. The integration of the data using an orthorhombic unit cell yielded a total of 11,622 reflections to a maximum θ angle of 30.56° (0.70 Å resolution), of which 1582 were independent (average redundancy 7.346, completeness = 98.9%, Rint = 1.45%) and 1559 (98.55%) were greater than 2σ (F2). The final cell parameters of a = 6.303 (2) Å, b = 7.104 (2) Å, c = 11.627 (3) Å, α = β = γ = 90°, and volume = 520.6 (2) Å3 are based upon the refinement of the XYZ-centroids of 9792 reflections above 20 σ (I) with 6.721° < 2θ < 61.11°. Data were corrected for absorption effects using the multi-scan method (SADABS). The ratio of minimum to maximum apparent transmission was 0.927. The calculated minimum and maximum transmission coefficients (based on crystal size) were 0.9150 and 0.9560, respectively.
The final anisotropic full-matrix least-squares refinement on F2 with 76 variables converged at R1 = 1.61% for the observed data and wR2 = 4.64% for all data. The goodness of fit was 1.167. The largest peak in the final difference electron-density synthesis was 0.400 e3, and the largest hole was −0.159 e3, with an RMS deviation of 0.052 e3. On the basis of the final model, the calculated density was 1.800 g/cm3 and F(000), 296 e.

Supplementary Materials

CIF and checkCIF reports for the crystal structure of 2, Figure S1: 1H NMR spectrum of 2 (400 MHz, D2O); Figure S2: 1H {31P} NMR spectrum of 2 (400 MHz, D2O); Figure S3: 13C NMR spectrum of 2 (62.9 MHz, D2O); Figure S4: 31P {1H} NMR spectrum of 2 (162 MHz, D2O); Figure S5: IR spectrum of 2 (KBr disk); Figure S6: Electrospray mass spectrum of 2.

Author Contributions

Conceptualization, P.B.W.; methodology, P.B.W., M.M., P.B., and I.A.; investigation, P.B.W., M.M., P.B., C.E.W., and I.A.; writing—original draft preparation, P.B.W.; writing—review and editing, P.B.W. and I.A.; visualization, P.B.W., M.M., and I.A.; supervision, P.B.W.; project administration, P.B.W. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Data Availability Statement

Crystallographic data (excluding structure factors) for the acid 2 are available in the Supplementary Materials of this paper and were deposited at the Cambridge Crystallographic Data Centre as supplementary publication no. CCDC 2381816. Copies of the data can be obtained, free of charge, upon application to CCDC, 12 Union Road, Cambridge CB2 1EZ, UK (fax: +44-(0)1223-336033 or e-mail: [email protected]).

Acknowledgments

We are grateful to the EPSRC NMSF at Swansea University for mass spectrometry and to the European Union for enabling P.B. to participate in the ERASMUS student exchange programme.

Conflicts of Interest

The authors declare no conflicts of interest.

References

  1. Duhamel, S.; Diaz, J.M.; Adams, J.C.; Djaoudi, K.; Steck, V.; Waggoner, E.M. Phosphorus as an integral component of global marine geochemistry. Nat. Geosc. 2021, 14, 359–368. [Google Scholar] [CrossRef]
  2. McSorley, F.R.; Wyatt, P.B.; Martinez, A.; DeLong, E.F.; Hove-Jensen, B.; Zechel, D.L. PhnY and PhnZ comprise a new oxidative pathway for enzymatic cleavage of a carbon-phosphorus bond. J. Am. Chem. Soc. 2012, 134, 8364–8367. [Google Scholar] [CrossRef] [PubMed]
  3. Van Staalduinen, L.M.; McSorley, F.R.; Schiessl, K.; Séguin, J.; Wyatt, P.B.; Hammerschmidt, F.; Zechel, D.L.; Jia, Z. Crystal structure of Phnz in complex with substrate reveals a di-iron oxygenase mechanism for catabolism of organophosphates. Proc. Natl. Acad. Sci. USA 2014, 111, 5171–5176. [Google Scholar] [CrossRef] [PubMed]
  4. Kolodiazhnyi, O.I.; Kolodiazhna, A.O. Stereoselective syntheses of organophosphorus compounds. Symmetry 2024, 16, 342. [Google Scholar] [CrossRef]
  5. Wyatt, P.B.; Blakskjær, P. An enantioselective synthesis of (R)-2-amino-1-hydroxyethylphosphonic acid by hydrolytic kinetic resolution of (±)-diethyl oxiranephosphonate. Tetrahedron Lett. 1999, 40, 6481–6483. [Google Scholar] [CrossRef]
  6. Tokunaga, M.; Larrow, J.F.; Kakiuchi, F.; Jacobsen, E.N. Asymmetric catalysis with water: Efficient kinetic resolution of terminal epoxides by means of catalytic hydrolysis. Science 1997, 277, 936–938. [Google Scholar] [CrossRef] [PubMed]
  7. Hammerschmidt, F.; Völlenkle, H. Absolute Konfiguration der (2-Amino-1-hydroxyethyl)phosphonsäure aus Ancanthamoeba castellanii (Neff)—Darstellung der Phosphonsaure-Analoga von (+)- und (−)- Serin. Liebigs Ann. Chem. 1989, 577–583. [Google Scholar] [CrossRef]
  8. Hammerschmidt, F.; Lindner, W.; Wuggenig, F.; Zarble, E. Enzymes in organic chemistry. Part 10: Chemo-enzymatic synthesis of L-phosphaserine and L-phosphaisoserine and enantioseparation of amino-hydroxyethylphosphonic acids by non-aqueous capillary electrophoresis with quinine carbamate as chiral pair agent. Tetrahedron Asymmetry 2000, 11, 2955–2964. [Google Scholar] [CrossRef]
  9. Pallitsch, K.; Happl, B.; Stieger, C. Determination of the absolute configuration of (−)-hydroxynitralaphos and related biosynthetic questions. Chem. Eur. J. 2017, 23, 15655–15665. [Google Scholar] [CrossRef] [PubMed]
  10. Okaya, Y. Crystal structure of the stable modification of 2-aminoethylphosphonic acid, β-ciliatine. Acta Crystallogr. 1966, 20, 712–715. [Google Scholar] [CrossRef]
  11. Crawczyk, H.; Albrecht, L.; Wojciechowski, J.; Wolf, W.M. Synthesis and crystal structure of 1-(aminomethyl)vinylphosphonic acid. Tetrahedron 2008, 64, 5051–5054. [Google Scholar] [CrossRef]
  12. Chen, S.-P.; Zhang, Y.-Q.; Hu, L.; He, H.-Z.; Yuan, L.-J. Hydrogen-bonded assembly of aminophosphonic anions: Different 1D, 2D and 3D supramolecular architectures. CrystEngComm 2010, 12, 3327–3336. [Google Scholar] [CrossRef]
  13. Bogomilova, A.; Hägele, G.; Troev, K.; Wagner, E.; Günther, M. Hydrogen bonding in α-aminophosphonic acids. Phosphorus Sulfur Relat. Elem. 2012, 187, 165–180. [Google Scholar] [CrossRef]
  14. Wanat, W.; Dziuk, B.; Kafarski, P. New crystal structures of α-aminophosphonic acid analogues of phenylglycine. Struct. Chem. 2020, 31, 1197–1209. [Google Scholar] [CrossRef]
  15. Flack, H.D. On enantiomorph-polarity estimation. Acta. Crystallogr. 1983, A39, 876–881. [Google Scholar] [CrossRef]
  16. Sturtz, G.; Pondaven-Raphalen, A. Etude de la réaction de cyclisation des halohydrines-1,2-éthyl phosphonates de diéthyle pour l’obtention de l’époxy-1,2 éthyl phosphonate de diéthyle. Phosphorus Sulfur Relat. Elem. 1984, 20, 35–47. [Google Scholar] [CrossRef]
Scheme 1. Catabolic pathway in which (R)-2-amino-1-hydroxyethylphosphonic acid 2 acts as a precursor to inorganic phosphate 3 following oxidation of 2-aminoethylphosphonic acid 1.
Scheme 1. Catabolic pathway in which (R)-2-amino-1-hydroxyethylphosphonic acid 2 acts as a precursor to inorganic phosphate 3 following oxidation of 2-aminoethylphosphonic acid 1.
Molbank 2024 m1888 sch001
Scheme 2. Chemical synthesis of (R)-2-amino-1-hydroxyethylphosphonic acid 2 from racemic epoxyphosphonate 5 using an enantioselective hydrolytic kinetic resolution [5].
Scheme 2. Chemical synthesis of (R)-2-amino-1-hydroxyethylphosphonic acid 2 from racemic epoxyphosphonate 5 using an enantioselective hydrolytic kinetic resolution [5].
Molbank 2024 m1888 sch002
Figure 1. Aminophosphonic acids 811 that have been characterized by X-ray crystallography [11,12,13,14].
Figure 1. Aminophosphonic acids 811 that have been characterized by X-ray crystallography [11,12,13,14].
Molbank 2024 m1888 g001
Figure 2. X-ray crystal structure of the acid 2 showing the atom numbering scheme. Thermal ellipsoids are shown at the 50% probability level.
Figure 2. X-ray crystal structure of the acid 2 showing the atom numbering scheme. Thermal ellipsoids are shown at the 50% probability level.
Molbank 2024 m1888 g002
Figure 3. Ball and stick representation showing hydrogen bonding (dashed lines) within the crystal structure of acid 2. Key: C, black; H, white; N, blue; O, red; P, green.
Figure 3. Ball and stick representation showing hydrogen bonding (dashed lines) within the crystal structure of acid 2. Key: C, black; H, white; N, blue; O, red; P, green.
Molbank 2024 m1888 g003
Table 1. Hydrogen bond distances (Å) and angles (°) for acid 2.
Table 1. Hydrogen bond distances (Å) and angles (°) for acid 2.
Donor-HAcceptor-HDonor-AcceptorAngle
N1-H4•••O20.912.232.951 (2)135.5
N1-H4•••O40.912.202.945 (1)139.1
N1-H5•••O20.911.842.730 (1)165.6
N1-H6•••O30.911.902.795 (1)165.9
O1-H7•••O30.841.692.503 (1)163.6
O4-H8•••O10.842.122.880 (1)150.1
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Motevalli, M.; Abrahams, I.; Blakskjær, P.; Wyatt, C.E.; Wyatt, P.B. (R)-2-Amino-1-hydroxyethylphosphonic Acid. Molbank 2024, 2024, M1888. https://doi.org/10.3390/M1888

AMA Style

Motevalli M, Abrahams I, Blakskjær P, Wyatt CE, Wyatt PB. (R)-2-Amino-1-hydroxyethylphosphonic Acid. Molbank. 2024; 2024(4):M1888. https://doi.org/10.3390/M1888

Chicago/Turabian Style

Motevalli, Majid, Isaac Abrahams, Peter Blakskjær, Caroline E. Wyatt, and Peter B. Wyatt. 2024. "(R)-2-Amino-1-hydroxyethylphosphonic Acid" Molbank 2024, no. 4: M1888. https://doi.org/10.3390/M1888

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop