Open AccessReview
Label-Free Aptasensors for the Detection of Mycotoxins
by
Amina Rhouati 1,2, Gaelle Catanante 1, Gilvanda Nunes 3, Akhtar Hayat 1,4,* and Jean-Louis Marty 1,*
1
BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France
2
Ecole Nationale Supérieure de Biotechnologie, Constantine 25100, Algeria
3
Technological Chemistry Department, Federal University of Maranhão, CCET/UFMA, Av. Portugueses, Cidade Universitária do Canga, 65080-040 São Luis, Brazil
4
Interdisciplinary Research Centre in Biomedical Materials (IRCBM) COMSATS Institute of Information Technology (CIIT), Lahore 54000, Pakistan
Cited by 82 | Viewed by 11218
Abstract
Various methodologies have been reported in the literature for the qualitative and quantitative monitoring of mycotoxins in food and feed samples. Based on their enhanced specificity, selectivity and versatility, bio-affinity assays have inspired many researchers to develop sensors by exploring bio-recognition phenomena. However,
[...] Read more.
Various methodologies have been reported in the literature for the qualitative and quantitative monitoring of mycotoxins in food and feed samples. Based on their enhanced specificity, selectivity and versatility, bio-affinity assays have inspired many researchers to develop sensors by exploring bio-recognition phenomena. However, a significant problem in the fabrication of these devices is that most of the biomolecules do not generate an easily measurable signal upon binding to the target analytes, and signal-generating labels are required to perform the measurements. In this context, aptamers have been emerged as a potential and attractive bio-recognition element to design label-free aptasensors for various target analytes. Contrary to other bioreceptor-based approaches, the aptamer-based assays rely on antigen binding-induced conformational changes or oligomerization states rather than binding-assisted changes in adsorbed mass or charge. This review will focus on current designs in label-free conformational switchable design strategies, with a particular focus on applications in the detection of mycotoxins.
Full article
►▼
Show Figures