This study investigated the changes in the acoustic emission (AE) activity emitted in fiber-reinforced concrete (FRC) under flexure at two temperatures (25 °C and −20 °C). Seven concrete mixtures were developed with different water-binder ratios (w/b) (0.4 and 0.55), different fiber materials (steel fiber (SF) and synthetic polypropylene fiber (Syn-PF)), different fiber lengths (19 mm and 38 mm), and various Syn-PF contents (0%, 0.2%, and 1%). Prisms with dimensions of 100 × 100 × 400 mm from each mixture underwent a four-point monotonic flexure load while collecting the emitted acoustic waves via attached AE sensors. AE parameter-based analyses, including
b-value, improved
b-value (
Ib-value), intensity, and rise time/average signal amplitude (RA) analyses, were performed using the raw AE data to highlight the change in the AE activity associated with different stages of damage (micro- and macro-cracking). The results showed that the number of hits, average frequency, cumulative signal strength (CSS), and energy were higher for the waves released at −20 °C compared to those obtained at 25 °C. The onset of the first visible micro- and macro-cracks was noticed to be associated with a significant spike in CSS, historic index (
H (t)), severity (
Sr) curves, a noticeable dip in the
b-value curve, and a compression in bellows/fluctuations of the
Ib-value curve for both testing temperatures. In addition, time and load thresholds of micro- and macro-cracks increased when samples were cooled down and tested at −20 °C, especially in the mixtures with higher w/b, longer fibers, and lower fiber content. This improvement in mechanical performance and cracking threshold limits was associated with higher AE activity in terms of an overall increase in CSS,
Sr, and
H (t) values and an overall reduction in
b-values. In addition, varying the concrete mixture design parameters, including the w/b ratio as well as fiber type, content, and length, showed a significant impact on the flexural behavior and the AE activity of the tested mixtures at both temperatures (25 °C and −20 °C). Intensity and RA analysis parameters allowed the development of two charts to characterize the detected AE events, whether associated with micro- and macro-cracks considering the temperature effect.
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