A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Instrumentation
2.3. Synthesis of Biotinylated Linker: N-Dithiooctanoylamidohexyl-5-Biotinylpentanamide (LHB)
2.4. QCM Setup and Detection Test
2.4.1. QCM Immunosensor Assembly
2.4.2. Procedure for the Detection of DPSCs
3. Results and Discussion
3.1. Derivatization of the Gold Surface for QCM Immunosensor Construction
3.2. Immobilization of Anti-CD34 Antibodies on QCM Surface and Test for CD34 Protein Binding
3.3. Stem Cell Detection by QCM-Immunosensor
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Layer | MW (Da) | Δf (Hz) | Δm (ng) | mol (pmol) | N |
---|---|---|---|---|---|
Avidin | 66,000 | 207 | 145 | 2.20 | 1.32 × 1012 |
Antibody | 150,000 | 261 | 184 | 1.23 | 0.74 × 1012 |
Starting Frequency | Avidin | Anti-CD34 | Stem Cell | |
---|---|---|---|---|
Average value (Hz) | 0 | 203 | 485 | 684 |
Median (Hz) | −0.12 | 229 | 506 | 676 |
Standard Deviation (Hz) | 1.2 | 62 | 32 | 40 |
Mean Absolute Deviation (Hz) | 1.3 | 56 | 30 | 34 |
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Maglio, O.; Costanzo, S.; Cercola, R.; Zambrano, G.; Mauro, M.; Battaglia, R.; Ferrini, G.; Nastri, F.; Pavone, V.; Lombardi, A. A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction. Sensors 2017, 17, 2747. https://doi.org/10.3390/s17122747
Maglio O, Costanzo S, Cercola R, Zambrano G, Mauro M, Battaglia R, Ferrini G, Nastri F, Pavone V, Lombardi A. A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction. Sensors. 2017; 17(12):2747. https://doi.org/10.3390/s17122747
Chicago/Turabian StyleMaglio, Ornella, Salvatore Costanzo, Rosaria Cercola, Gerardo Zambrano, Marco Mauro, Raffaele Battaglia, Gianluca Ferrini, Flavia Nastri, Vincenzo Pavone, and Angela Lombardi. 2017. "A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction" Sensors 17, no. 12: 2747. https://doi.org/10.3390/s17122747
APA StyleMaglio, O., Costanzo, S., Cercola, R., Zambrano, G., Mauro, M., Battaglia, R., Ferrini, G., Nastri, F., Pavone, V., & Lombardi, A. (2017). A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction. Sensors, 17(12), 2747. https://doi.org/10.3390/s17122747