A Multichannel Fluorescent Array Sensor for Discrimination of Different Types of Drug-Induced Kidney Injury

Sun, Kunhui; Wang, Bing; Lin, Jiaoli; Han, Lei; Li, Meifang; Wang, Ping; Yu, Xiean; Tian, Jiangwei

doi:10.3390/s23136114

Open AccessArticle

A Multichannel Fluorescent Array Sensor for Discrimination of Different Types of Drug-Induced Kidney Injury

by

Kunhui Sun

^1,2,†,

Bing Wang

^2,†,

Jiaoli Lin

¹,

Lei Han

¹,

Meifang Li

²,

Ping Wang

²,

Xiean Yu

^2,*

and

Jiangwei Tian

^1,*

¹

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China

²

NMPA Key Laboratory for Bioequivalence Research of Generic Drug Evaluation, Shenzhen Institute for Drug Control, Shenzhen 518057, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Sensors 2023, 23(13), 6114; https://doi.org/10.3390/s23136114

Submission received: 10 May 2023 / Revised: 24 June 2023 / Accepted: 26 June 2023 / Published: 3 July 2023

(This article belongs to the Special Issue Fluorescence Sensors for Biological and Medical Applications)

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Abstract

The differences in urinary proteins could provide a novel opportunity to distinguish the different types of drug-induced kidney injury (DIKI). In this research, Au nanoparticles–polyethyleneimine (AuNPs–PEI) and the three fluorophore-labeled proteins (FLPs) have been constructed as a multichannel fluorescent array sensor via electrostatic interaction, which was used to detect the subtle changes in urine collected from the pathological state of DIKI. Once the urine from different types of DIKI was introduced, the binding equilibrium between AuNPs–PEI and FLPs would be broken due to the competitive binding of urinary protein, and the corresponding fluorescence response pattern would be generated. Depending on the different fluorescence response patterns, the different types of DIKI were successfully identified by principal component analysis (PCA) and linear discriminant analysis (LDA). Accordingly, the strategy was expected to be a powerful technique for evaluating the potential unclear mechanisms of nephrotoxic drugs, which would provide a promising method for screening potential renal-protective drugs.

Keywords: multichannel fluorescent array sensor; drug-induced kidney injury; Au nanoparticles–polyethyleneimine (AuNPs–PEI); fluorophore-labeled proteins

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MDPI and ACS Style

Sun, K.; Wang, B.; Lin, J.; Han, L.; Li, M.; Wang, P.; Yu, X.; Tian, J. A Multichannel Fluorescent Array Sensor for Discrimination of Different Types of Drug-Induced Kidney Injury. Sensors 2023, 23, 6114. https://doi.org/10.3390/s23136114

AMA Style

Sun K, Wang B, Lin J, Han L, Li M, Wang P, Yu X, Tian J. A Multichannel Fluorescent Array Sensor for Discrimination of Different Types of Drug-Induced Kidney Injury. Sensors. 2023; 23(13):6114. https://doi.org/10.3390/s23136114

Chicago/Turabian Style

Sun, Kunhui, Bing Wang, Jiaoli Lin, Lei Han, Meifang Li, Ping Wang, Xiean Yu, and Jiangwei Tian. 2023. "A Multichannel Fluorescent Array Sensor for Discrimination of Different Types of Drug-Induced Kidney Injury" Sensors 23, no. 13: 6114. https://doi.org/10.3390/s23136114

APA Style

Sun, K., Wang, B., Lin, J., Han, L., Li, M., Wang, P., Yu, X., & Tian, J. (2023). A Multichannel Fluorescent Array Sensor for Discrimination of Different Types of Drug-Induced Kidney Injury. Sensors, 23(13), 6114. https://doi.org/10.3390/s23136114

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A Multichannel Fluorescent Array Sensor for Discrimination of Different Types of Drug-Induced Kidney Injury

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