An Electrochemical Nucleic Acid Biosensor for Triple-Negative Breast Cancer Biomarker Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
- miRNA-10b: 5′-UAC CCU GUA GAA CCG AAU UUG UG-3′;
- Anti-miRNA-10b (DNA probe strand): 5′-NH2-C6-CAC AAA TTC GGT TCT ACA GGG TA-3′;
- Non-Target miRNA 1: 5′-GGC CCA CUA GCA CCU AAC UGG UA-3′;
- Non-Target miRNA 2: 5′-UAG ACU GUA CAA CUG ACU UUG GG-3′.
2.2. Methods
2.2.1. Fabrication of the ssDNA-Immobilized Electrode
2.2.2. Hybridization of miRNA-10b
2.2.3. Electrochemical Characterization
2.2.4. Biosensor Testing and Validation
3. Results and Discussion
3.1. The Fabrication and Working Principle of the Biosensor
3.2. Optimization of Experimental Conditions
3.3. The Sensitivity of the Biosensor
3.4. The Specificity of the Biosensor
3.5. The Stability of the Biosensor
3.6. Validating the Sensitivity of the Biosensor with a Synthetic Serum Sample
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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miRNA10b Concentration | Expected Median | Expected Range | Actual Median | Actual Range |
---|---|---|---|---|
10−7 M | 174 | 163–186 | 175 | 125–265 |
10−8 M | 128 | 109–146 | 131 | 90–166 |
10−9 M | 92 | 80–107 | 78.5 | 76–81 |
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Hansen, L.; Nagdeve, S.N.; Suganthan, B.; Ramasamy, R.P. An Electrochemical Nucleic Acid Biosensor for Triple-Negative Breast Cancer Biomarker Detection. Sensors 2024, 24, 5747. https://doi.org/10.3390/s24175747
Hansen L, Nagdeve SN, Suganthan B, Ramasamy RP. An Electrochemical Nucleic Acid Biosensor for Triple-Negative Breast Cancer Biomarker Detection. Sensors. 2024; 24(17):5747. https://doi.org/10.3390/s24175747
Chicago/Turabian StyleHansen, Lexi, Sanket Naresh Nagdeve, Baviththira Suganthan, and Ramaraja P. Ramasamy. 2024. "An Electrochemical Nucleic Acid Biosensor for Triple-Negative Breast Cancer Biomarker Detection" Sensors 24, no. 17: 5747. https://doi.org/10.3390/s24175747