cfDNA Sequencing: Technological Approaches and Bioinformatic Issues

Bohers, Elodie; Viailly, Pierre-Julien; Jardin, Fabrice

doi:10.3390/ph14060596

Open AccessFeature PaperReview

cfDNA Sequencing: Technological Approaches and Bioinformatic Issues

by

Elodie Bohers

^*

,

Pierre-Julien Viailly

and

Fabrice Jardin

INSERM U1245, Henri Becquerel Center, IRIB, Normandy University, 76000 Rouen, France

^*

Author to whom correspondence should be addressed.

Pharmaceuticals 2021, 14(6), 596; https://doi.org/10.3390/ph14060596

Submission received: 14 May 2021 / Revised: 18 June 2021 / Accepted: 18 June 2021 / Published: 21 June 2021

(This article belongs to the Special Issue Cell-Free DNA for the Management of Lymphoma)

Download

Browse Figure

Versions Notes

Abstract

In the era of precision medicine, it is crucial to identify molecular alterations that will guide the therapeutic management of patients. In this context, circulating tumoral DNA (ctDNA) released by the tumor in body fluids, like blood, and carrying its molecular characteristics is becoming a powerful biomarker for non-invasive detection and monitoring of cancer. Major recent technological advances, especially in terms of sequencing, have made possible its analysis, the challenge still being its reliable early detection. Different parameters, from the pre-analytical phase to the choice of sequencing technology and bioinformatic tools can influence the sensitivity of ctDNA detection.

Keywords: cell-free DNA; circulating tumoral DNA; sequencing technologies; bioinformatics

Share and Cite

MDPI and ACS Style

Bohers, E.; Viailly, P.-J.; Jardin, F. cfDNA Sequencing: Technological Approaches and Bioinformatic Issues. Pharmaceuticals 2021, 14, 596. https://doi.org/10.3390/ph14060596

AMA Style

Bohers E, Viailly P-J, Jardin F. cfDNA Sequencing: Technological Approaches and Bioinformatic Issues. Pharmaceuticals. 2021; 14(6):596. https://doi.org/10.3390/ph14060596

Chicago/Turabian Style

Bohers, Elodie, Pierre-Julien Viailly, and Fabrice Jardin. 2021. "cfDNA Sequencing: Technological Approaches and Bioinformatic Issues" Pharmaceuticals 14, no. 6: 596. https://doi.org/10.3390/ph14060596

APA Style

Bohers, E., Viailly, P.-J., & Jardin, F. (2021). cfDNA Sequencing: Technological Approaches and Bioinformatic Issues. Pharmaceuticals, 14(6), 596. https://doi.org/10.3390/ph14060596

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

cfDNA Sequencing: Technological Approaches and Bioinformatic Issues

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI