Next Article in Journal
Benzydamine—An Affordable Over-the-Counter Drug with Psychoactive Properties—From Chemical Structure to Possible Pharmacological Properties
Previous Article in Journal
Gut Microbiota in Anxiety and Depression: Unveiling the Relationships and Management Options
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pharmaceuticals
Volume 16
Issue 4
10.3390/ph16040564
pharmaceuticals-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm of Staphylococcus aureus and Enterococcus faecalis

by
Salim Manoharadas
1,2,*,
Naushad Ahmad
2,3,
Mohammad Altaf
2,3,
Abdulwahed Fahad Alrefaei
4 and
Basel F. Al-Rayes
2
1
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2454, Riyadh 11451, Saudi Arabia
2
Central Laboratory RM 63AA, College of Science, King Saud University, P.O. Box 2454, Riyadh 11451, Saudi Arabia
3
Department of Chemistry, College of Science, King Saud University, P.O. Box 2454, Riyadh 11451, Saudi Arabia
4
Department of Zoology, College of Science, King Saud University, P.O. Box 2454, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2023, 16(4), 564; https://doi.org/10.3390/ph16040564
Submission received: 23 March 2023 / Revised: 5 April 2023 / Accepted: 8 April 2023 / Published: 10 April 2023
(This article belongs to the Special Issue Recent Advances in Antimicrobial Nanodrugs 2023)
Browse Figures
Versions Notes

Abstract

Multidrug-resistant bacterial infections are on the rise around the world. Chronic infections caused by these pathogens through biofilm mediation often complicate the situation. In natural settings, biofilms are often formed with different species of bacteria existing synergistically or antagonistically. Biofilms on diabetic foot ulcers are formed predominantly by two opportunistic pathogens, Staphylococcus aureus and Enterococcus faecalis. Bacteriophages and phage-based proteins, including endolysins, have been found to be active against biofilms. In this study, we evaluated the activity of two engineered enzybiotics either by themselves or as a combination against a dual biofilm formed by S. aureus and E. faecalis in an inert glass surface. An additive effect in rapidly disrupting the preformed dual biofilm was observed with the cocktail of proteins, in comparison with mono treatment. The cocktail-treated biofilms were dispersed by more than 90% within 3 h of treatment. Apart from biofilm disruption, bacterial cells embedded in the biofilm matrix were also effectively reduced by more than 90% within 3 h of treatment. This is the first instance where a cocktail of engineered enzybiotics has been effectively used to impede the structural integrity of a dual biofilm.
Keywords: enzybiotics; biofilm; antimicrobials; Staphylococcus aureus; Enterococcus faecalis; diabetic foot ulcers enzybiotics; biofilm; antimicrobials; Staphylococcus aureus; Enterococcus faecalis; diabetic foot ulcers

Share and Cite

MDPI and ACS Style

Manoharadas, S.; Ahmad, N.; Altaf, M.; Alrefaei, A.F.; Al-Rayes, B.F. An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm of Staphylococcus aureus and Enterococcus faecalis. Pharmaceuticals 2023, 16, 564. https://doi.org/10.3390/ph16040564

AMA Style

Manoharadas S, Ahmad N, Altaf M, Alrefaei AF, Al-Rayes BF. An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm of Staphylococcus aureus and Enterococcus faecalis. Pharmaceuticals. 2023; 16(4):564. https://doi.org/10.3390/ph16040564

Chicago/Turabian Style

Manoharadas, Salim, Naushad Ahmad, Mohammad Altaf, Abdulwahed Fahad Alrefaei, and Basel F. Al-Rayes. 2023. "An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm of Staphylococcus aureus and Enterococcus faecalis" Pharmaceuticals 16, no. 4: 564. https://doi.org/10.3390/ph16040564

APA Style

Manoharadas, S., Ahmad, N., Altaf, M., Alrefaei, A. F., & Al-Rayes, B. F. (2023). An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm of Staphylococcus aureus and Enterococcus faecalis. Pharmaceuticals, 16(4), 564. https://doi.org/10.3390/ph16040564

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop