Effects of the DL76 Antagonist/Inverse Agonist of Histamine H3 Receptors on Experimental Periodontitis in Rats: Morphological Studies
Abstract
:1. Introduction
2. Results
2.1. Histopathological Results
2.2. IHC Results
2.3. Radiological Results
3. Discussion
4. Limitations of the Study
5. Materials and Methods
5.1. Characteristics of the Inverse Agonist/Antagonist of the Histamine H3 Receptor, DL76
5.2. Immunohistochemical Analysis
5.3. X-ray Pictures of the Rat Maxillas
5.4. Linear Measurement of Alveolar Bone Loss
5.5. Description of the Applied Statistical Methods
5.6. Statistics concerning Bone Loss in Rat Maxillas
5.7. Statistics concerning IHC Variables
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | M | Me | SD | Min | Max | Statistical Test Result * | |
---|---|---|---|---|---|---|---|
RANK-e | SL | 55% | 67.5% | 28.64% | 10% | 80% | χ2(3) = 2.07; p = 0.56 |
SR | 71.67% | 72.5% | 6.83% | 60% | 80% | ||
DLL | 60.83% | 65% | 13.57% | 40% | 75% | ||
DLR | 66.67% | 70% | 11.69% | 50% | 80% | ||
RANKL-e | SL | 0.83% | 0% | 2.04% | 0% | 5% | χ2(3) = 9.3; p = 0.03 |
SR | 15% | 20% | 12.25% | 0% | 25% | ||
DLL | 0% | 0% | 0% | 0% | 0% | ||
DLR | 0.83% | 0% | 2.04% | 0% | 5% | ||
OPG-e | SL | 0% | 0% | 0% | 0% | 0% | χ2(3) = 10.77; p = 0.01 |
SR | 5% | 5% | 4.47% | 0% | 10% | ||
DLL | 0% | 0% | 0% | 0% | 0% | ||
DLR | 0.83% | 0% | 2.04% | 0% | 5% |
Variable | n (%) | Statistical Test Result * | ||||
---|---|---|---|---|---|---|
Lack of Expression | Low Expression | Average Expression | High Expression | |||
RANK-e | SL | 0 (0%) | 1 (16,7%) | 1 (16.7%) | 4 (66.6%) | χ2(6) = 5.58; p = 0.47 |
SR | 0 (0%) | 0 (0%) | 0 (0%) | 6 (100%) | ||
DLL | 0 (0%) | 0 (0%) | 2 (33.3%) | 4 (66.7%) | ||
DLR | 0 (0%) | 0 (0%) | 1 (16.7%) | 5 (83.3%) | ||
RANK-s | SL | 0 (0%) | 6 (100%) | 0 (0%) | 0 (0%) | χ2(3) = 9.78; p = 0.02 |
SR | 0 (0%) | 6 (100%) | 0 (0%) | 0 (0%) | ||
DLL | 4 (66.7%) | 2 (33.3%) | 0 (0%) | 0 (0%) | ||
DLR | 2 (33.3%) | 4 (66.7%) | 0 (0%) | 0 (0%) | ||
RANKL-e | SL | 5 (83.3%) | 1 (16.7%) | 0 (0%) | 0 (0%) | χ2(3) = 8; p = 0.046 |
SR | 2 (33.3%) | 4 (66.7%) | 0 (0%) | 0 (0%) | ||
DLL | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | ||
DLR | 5 (83.3%) | 1 (16.7%) | 0 (0%) | 0 (0%) | ||
RANKL-s | SL | 5 (83.3%) | 1 (16.7) | 0 (0%) | 0 (0%) | χ2(3) = 2.18; p = 0.54 |
SR | 5 (83.3%) | 1 (16.7) | 0 (0%) | 0 (0%) | ||
DLL | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | ||
DLR | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | ||
OPG-e | SL | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | χ2(3) = 10.86; p = 0.01 |
SR | 2 (33.3%) | 4 (66.7%) | 0 (0%) | 0 (0%) | ||
DLL | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | ||
DLR | 5 (83.3%) | 1 (16.7%) | 0 (0%) | 0 (0%) | ||
OPG-s | SL | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | χ2(3) = 10.29; p = 0.02 |
SR | 3 (50%) | 3 (50%) | 0 (0%) | 0 (0%) | ||
DLL | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | ||
DLR | 6 (100%) | 0 (0%) | 0 (0%) | 0 (0%) |
Variable | M. | Me | SD | Min | Max | Statistical Test Result * | |
---|---|---|---|---|---|---|---|
Mesial space | SL | 0.39 | 0.41 | 0.09 | 0.25 | 0.51 | χ2 (3) = 20.25; p < 0.001 |
SR | 0.79 | 0.82 | 0.17 | 0.54 | 0.98 | ||
DLL | 0 | 0 | 0 | 0 | 0 | ||
DLR | 0.18 | 0.23 | 0.16 | 0 | 0.33 | ||
Distal space | SL | 0.37 | 0.15 | 0.14 | 0.25 | 0.64 | χ2 (3) = 20.85; p < 0.001 |
SR | 0.84 | 0.8 | 0.2 | 0.58 | 1.13 | ||
DLL | 0 | 0 | 0 | 0 | 0 | ||
DLR | 0.09 | 0.08 | 0.1 | 0 | 0.2 |
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Geremek, M.; Drozdzowska, B.; Łażewska, D.; Kieć-Kononowicz, K.; Jochem, J. Effects of the DL76 Antagonist/Inverse Agonist of Histamine H3 Receptors on Experimental Periodontitis in Rats: Morphological Studies. Pharmaceuticals 2024, 17, 792. https://doi.org/10.3390/ph17060792
Geremek M, Drozdzowska B, Łażewska D, Kieć-Kononowicz K, Jochem J. Effects of the DL76 Antagonist/Inverse Agonist of Histamine H3 Receptors on Experimental Periodontitis in Rats: Morphological Studies. Pharmaceuticals. 2024; 17(6):792. https://doi.org/10.3390/ph17060792
Chicago/Turabian StyleGeremek, Mariusz, Bogna Drozdzowska, Dorota Łażewska, Katarzyna Kieć-Kononowicz, and Jerzy Jochem. 2024. "Effects of the DL76 Antagonist/Inverse Agonist of Histamine H3 Receptors on Experimental Periodontitis in Rats: Morphological Studies" Pharmaceuticals 17, no. 6: 792. https://doi.org/10.3390/ph17060792