TnP and AHR-CYP1A1 Signaling Crosstalk in an Injury-Induced Zebrafish Inflammation Model
Abstract
:1. Introduction
2. Results and Discussion
2.1. TnP Disrupts the AHR-CYP Signaling Pathway
2.2. The Role of AHR in the Therapeutic Effect of TnP
2.3. Comparisons between Zebrafish and Human CYP1A1 and TnP-CYP1A1 Docking
3. Materials and Methods
3.1. Reagents and Chemicals
3.2. CYP1A1 Activity In Vitro Inhibition Assay by TnP
3.3. Zebrafish Maintenance and Cyp1a Reporter Experiments
3.4. Quantitative Real-Time PCR
3.5. Experimental Settings for Transcriptome Analysis
3.5.1. Ahr2 Knockdown, TnP Treatment, and Inflammation Model
3.5.2. RNA Quantification and Library Preparation
3.5.3. Clustering and Sequencing
3.6. Data Analysis
3.6.1. Quality Control and Trimming
3.6.2. Reads Mapping, Quantification of Mapped Transcripts, Gene Expression Matrix, and Transcript Annotation
3.6.3. Reads Grouping
3.6.4. Differential Expression and Gene Ontology Enrichment Analysis
3.7. In Silico Analysis
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Disner, G.R.; Fernandes, T.A.d.M.; Nishiyama-Jr, M.Y.; Lima, C.; Wincent, E.; Lopes-Ferreira, M. TnP and AHR-CYP1A1 Signaling Crosstalk in an Injury-Induced Zebrafish Inflammation Model. Pharmaceuticals 2024, 17, 1155. https://doi.org/10.3390/ph17091155
Disner GR, Fernandes TAdM, Nishiyama-Jr MY, Lima C, Wincent E, Lopes-Ferreira M. TnP and AHR-CYP1A1 Signaling Crosstalk in an Injury-Induced Zebrafish Inflammation Model. Pharmaceuticals. 2024; 17(9):1155. https://doi.org/10.3390/ph17091155
Chicago/Turabian StyleDisner, Geonildo Rodrigo, Thales Alves de Melo Fernandes, Milton Yutaka Nishiyama-Jr, Carla Lima, Emma Wincent, and Monica Lopes-Ferreira. 2024. "TnP and AHR-CYP1A1 Signaling Crosstalk in an Injury-Induced Zebrafish Inflammation Model" Pharmaceuticals 17, no. 9: 1155. https://doi.org/10.3390/ph17091155