Gastrodin Alleviates Angiotensin II-Induced Hypertension and Myocardial Apoptosis via Inhibition of the PRDX2/p53 Pathway In Vivo and In Vitro
Abstract
:1. Introduction
2. Results
2.1. Gastrodin Reduces the Blood Pressure in Ang II-Induced Hypertensive Mice
2.2. Gastrodin Attenuates Cardiac Dysfunction and Pathological Damage in Ang II-Induced Hypertensive Mice
2.3. The Differentially Expressed Transcripts (DETs) and Pathway Enrichment in the Cardiac Tissues of Ang II-Induced Hypertensive Mice Following Gastrodin Treatment
2.4. Gastrodin Reduces Ang II-Induced Myocardial Apoptosis In Vivo
2.5. Gastrodin Attenuates Ang II-Induced Apoptosis in H9c2 Cells
2.6. Gastrodin Inhibits PRDX2/p53 Pathway Activation in Ang II-Induced Hypertension In Vivo and In Vitro
3. Discussion
4. Materials and Methods
4.1. Reagents and Consumables
4.2. Animals and Experimental Protocols
4.3. Blood Pressure Measurement and Echocardiography
4.4. Determination of Heart Weight/Tibia Length Ratio and Hematoxylin and Eosin (H&E) Staining
4.5. Immunohistochemical (IHC) Analysis
4.6. TUNEL Staining
4.7. RNA Sequencing (RNA-Seq)
4.8. Cell Culture and Treatments
4.9. Hoechst Staining
4.10. Apoptosis Assay by Flow Cytometry
4.11. Western-Blot Analysis
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Group | Mean ± SD | Median | IQRs 1 (25%) | IQRs (75%) |
---|---|---|---|---|
Control | 104.55 ± 2.69 | 104.83 | 103.79 | 99.91 |
Ang II | 178.47 ± 10.96 | 174.54 | 172.60 | 169.09 |
Ang II + gastrodin | 126.34 ± 6.31 | 125.44 | 123.28 | 117.89 |
Group | Mean ± SD | Median | IQRs 1 (25%) | IQRs (75%) |
---|---|---|---|---|
Control | 71.93 ± 2.68 | 72.37 | 71.27 | 67.18 |
Ang II | 154.04 ± 7.60 | 150.57 | 149.24 | 147.93 |
Ang II + gastrodin | 105.72 ± 7.34 | 104.85 | 99.47 | 98.00 |
Group | Mean ± SD | Median | IQRs 1 (25%) | IQRs (75%) |
---|---|---|---|---|
Control | 86.20 ± 1.74 | 86.68 | 85.46 | 83.27 |
Ang II | 164.21 ± 7.87 | 161.24 | 158.85 | 158.13 |
Ang II + gastrodin | 112.76 ± 7.59 | 111.90 | 106.89 | 104.33 |
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Xu, N.; Xie, Q.; Chen, Y.; Li, J.; Zhang, X.; Zheng, H.; Cheng, Y.; Wu, M.; Shen, A.; Wei, L.; et al. Gastrodin Alleviates Angiotensin II-Induced Hypertension and Myocardial Apoptosis via Inhibition of the PRDX2/p53 Pathway In Vivo and In Vitro. Pharmaceuticals 2024, 17, 1200. https://doi.org/10.3390/ph17091200
Xu N, Xie Q, Chen Y, Li J, Zhang X, Zheng H, Cheng Y, Wu M, Shen A, Wei L, et al. Gastrodin Alleviates Angiotensin II-Induced Hypertension and Myocardial Apoptosis via Inhibition of the PRDX2/p53 Pathway In Vivo and In Vitro. Pharmaceuticals. 2024; 17(9):1200. https://doi.org/10.3390/ph17091200
Chicago/Turabian StyleXu, Nanhui, Qiurong Xie, Youqin Chen, Jiapeng Li, Xiuli Zhang, Huifang Zheng, Ying Cheng, Meizhu Wu, Aling Shen, Lihui Wei, and et al. 2024. "Gastrodin Alleviates Angiotensin II-Induced Hypertension and Myocardial Apoptosis via Inhibition of the PRDX2/p53 Pathway In Vivo and In Vitro" Pharmaceuticals 17, no. 9: 1200. https://doi.org/10.3390/ph17091200