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Volume 12
Issue 3
10.21775/cimb.012.135
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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Green Technologies for Room Temperature Nucleic Acid Storage

by
Eunice Wan
1,
Matthew Akana
1,
Jennifer Pons
1,
Justin Chen
1,
Stacy Musone
1,
Pui-Yan Kwok
1,2,3 and
Wilson Liao
2,*
1
Cardiovascular Research Institute, University of California, San Francisco, 513 Parnassus Ave., San Francisco, CA 94143, USA
2
Department of Dermatology, University of California, San Francisco, 513 Parnassus Ave., San Francisco, CA 94143, USA
3
Institute for Human Genetics, University of California, San Francisco, 513 Parnassus Ave., San Francisco, CA 94143, USA
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2010, 12(3), 135-142; https://doi.org/10.21775/cimb.012.135
Submission received: 4 June 2009 / Revised: 14 June 2009 / Accepted: 11 August 2009 / Published: 2 October 2009

Abstract

Maintaining the long-term integrity of nucleic acids in the laboratory has traditionally required the use of freezers. However, novel nucleic acid stabilization technologies may allow for the storage of DNA and RNA at room temperature in a cost-effective, environmentally friendly manner. In this study, we evaluated two novel products for room temperature DNA storage: Biomatrica's DNA SampleMatrix technology and GenVault's GenTegra DNA technology. We compared the integrity and quality of DNA stored using these products against DNA stored in a -20° C freezer by performing downstream testing with short range PCR, long range PCR, DNA sequencing, and SNP microarrays. In addition, we tested Biomatrica's RNAstable product for its ability to preserve RNA at room temperature for use in a quantitative reverse transcription PCR assay.
Keywords: room temperature ; environmentally friendly; DNA storage; DNA SampleMatrix; GenVault; GenTegra; DNA; freezer; PCR; RNAstable; RNA room temperature ; environmentally friendly; DNA storage; DNA SampleMatrix; GenVault; GenTegra; DNA; freezer; PCR; RNAstable; RNA

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MDPI and ACS Style

Wan, E.; Akana, M.; Pons, J.; Chen, J.; Musone, S.; Kwok, P.-Y.; Liao, W. Green Technologies for Room Temperature Nucleic Acid Storage. Curr. Issues Mol. Biol. 2010, 12, 135-142. https://doi.org/10.21775/cimb.012.135

AMA Style

Wan E, Akana M, Pons J, Chen J, Musone S, Kwok P-Y, Liao W. Green Technologies for Room Temperature Nucleic Acid Storage. Current Issues in Molecular Biology. 2010; 12(3):135-142. https://doi.org/10.21775/cimb.012.135

Chicago/Turabian Style

Wan, Eunice, Matthew Akana, Jennifer Pons, Justin Chen, Stacy Musone, Pui-Yan Kwok, and Wilson Liao. 2010. "Green Technologies for Room Temperature Nucleic Acid Storage" Current Issues in Molecular Biology 12, no. 3: 135-142. https://doi.org/10.21775/cimb.012.135

APA Style

Wan, E., Akana, M., Pons, J., Chen, J., Musone, S., Kwok, P.-Y., & Liao, W. (2010). Green Technologies for Room Temperature Nucleic Acid Storage. Current Issues in Molecular Biology, 12(3), 135-142. https://doi.org/10.21775/cimb.012.135

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