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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Status and Prospects of Next-Generation Sequencing Technologies in Crop Plants

by
Tilak R. Sharma
*,
Basavantraya N. Devanna
,
Kanti Kiran
,
Pankaj K. Singh
,
Kirti Arora
,
Priyanka Jain
,
Ila M. Tiwari
,
Himanshu Dubey
,
Banita K. Saklani
,
Mandeep Kumari
,
Jyoti Singh
,
Rajdeep Jaswal
,
Ritu Kapoor
,
Deepak V. Pawar
,
Shruti Sinha
,
Deepak S. Bisht
,
Amolkumar U. Solanke
and
Tapan K. Mondal
ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2018, 27(1), 1-36; https://doi.org/10.21775/cimb.027.001
Submission received: 1 June 2017 / Revised: 6 July 2017 / Accepted: 2 August 2017 / Published: 8 September 2017
Versions Notes

Abstract

The history of DNA sequencing dates back to 1970s. During this period the two first generation nucleotide sequencing techniques were developed. Subsequently, Sanger's dideoxy method of sequencing gained popularity over Maxam and Gilbert's chemical method of sequencing. However, in the last decade, we have observed revolutionary changes in DNA sequencing technologies leading to the emergence of next-generation sequencing (NGS) techniques. NGS technologies have enhanced the throughput and speed of sequencing combined with bringing down the overall cost of the process over a time. The major applications of NGS technologies being genome sequencing and resequencing, transcriptomics, metagenomics in relation to plant-microbe interactions, exon and genome capturing, development of molecular markers and evolutionary studies. In this review, we present a broader picture of evolution of NGS tools, its various applications in crop plants, and future prospects of the technology for crop improvement.

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MDPI and ACS Style

Sharma, T.R.; Devanna, B.N.; Kiran, K.; Singh, P.K.; Arora, K.; Jain, P.; Tiwari, I.M.; Dubey, H.; Saklani, B.K.; Kumari, M.; et al. Status and Prospects of Next-Generation Sequencing Technologies in Crop Plants. Curr. Issues Mol. Biol. 2018, 27, 1-36. https://doi.org/10.21775/cimb.027.001

AMA Style

Sharma TR, Devanna BN, Kiran K, Singh PK, Arora K, Jain P, Tiwari IM, Dubey H, Saklani BK, Kumari M, et al. Status and Prospects of Next-Generation Sequencing Technologies in Crop Plants. Current Issues in Molecular Biology. 2018; 27(1):1-36. https://doi.org/10.21775/cimb.027.001

Chicago/Turabian Style

Sharma, Tilak R., Basavantraya N. Devanna, Kanti Kiran, Pankaj K. Singh, Kirti Arora, Priyanka Jain, Ila M. Tiwari, Himanshu Dubey, Banita K. Saklani, Mandeep Kumari, and et al. 2018. "Status and Prospects of Next-Generation Sequencing Technologies in Crop Plants" Current Issues in Molecular Biology 27, no. 1: 1-36. https://doi.org/10.21775/cimb.027.001

APA Style

Sharma, T. R., Devanna, B. N., Kiran, K., Singh, P. K., Arora, K., Jain, P., Tiwari, I. M., Dubey, H., Saklani, B. K., Kumari, M., Singh, J., Jaswal, R., Kapoor, R., Pawar, D. V., Sinha, S., Bisht, D. S., Solanke, A. U., & Mondal, T. K. (2018). Status and Prospects of Next-Generation Sequencing Technologies in Crop Plants. Current Issues in Molecular Biology, 27(1), 1-36. https://doi.org/10.21775/cimb.027.001

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