Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Curcumin-Loaded MSNs
2.2. Characterization of Curcumin Loaded MSNs
2.3. Cell Culture
2.4. Cell Viability Assessment
2.5. Evaluation of Expression Level of Bcl-2 and Bax
2.6. ROS Detection in Cancer Cells
2.7. Statistical Analysis
3. Results
3.1. Characterization of Cur-MSNs
3.2. Cytotoxicity Evaluation of Cur-MSNs in HN5 Cell Line
3.3. Expression Level of Bcl-2 and Bax
3.4. Detection of ROS
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Sequences | Product Length (bp) | Tm (°C) |
---|---|---|---|
Bax | F: 5′-TTTGCTTCAGGGTTTCATCCA-3′ R: 5′-CTCCATGTTACTGTCCAGTTCGT-3′ | 151 | 60 |
Bcl-2 | F: 5′-CCTGTGGATGACTGAGTACC-3′ R: 5′-GAGACAGCCAGGAGAAATCA-3′ | 128 | 60 |
GAPDH | F: 5′-AGCCACATCGCTCAGACAC-3′ R: 5′-GCCCAATACGACCAAATCC-3′ | 66 | 58 |
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Sharifi, S.; Dalir Abdolahinia, E.; Ghavimi, M.A.; Dizaj, S.M.; Aschner, M.; Saso, L.; Khan, H. Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5. Curr. Issues Mol. Biol. 2022, 44, 5247-5259. https://doi.org/10.3390/cimb44110357
Sharifi S, Dalir Abdolahinia E, Ghavimi MA, Dizaj SM, Aschner M, Saso L, Khan H. Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5. Current Issues in Molecular Biology. 2022; 44(11):5247-5259. https://doi.org/10.3390/cimb44110357
Chicago/Turabian StyleSharifi, Simin, Elaheh Dalir Abdolahinia, Mohammad Ali Ghavimi, Solmaz Maleki Dizaj, Michael Aschner, Luciano Saso, and Haroon Khan. 2022. "Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5" Current Issues in Molecular Biology 44, no. 11: 5247-5259. https://doi.org/10.3390/cimb44110357