Effect of Porcine Placental Extract Mixture on Alcohol-Induced Hepatotoxicity in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Experimental Groups
2.3. Experimental Procedure
2.4. Analytical Procedures
2.5. Histopathological Examination
2.6. Statistical Analysis
3. Results
3.1. Blood Chemistry Analysis
3.2. Effects of pPEM on Hepatic ADH and ALDH Activities
3.3. Effect of pPEM on Hepatic Pathology
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Test Samples | Total Nitrogen * | Amino Acids | Peptides |
---|---|---|---|
Enzymatic Extract | 46.8 mg/g | 42.5% | 57.5% |
Acid Extract | 35.8 mg/g | 81.2% | 18.8% |
pPEM (E/A = 1/3) | 39.0 mg/g | 62.0% | 38.0% |
Groups (n = 10)/ Parameters | LDH | TG | Albumin | ALP | Total Bilirubin | Cholesterol | Total Protein |
---|---|---|---|---|---|---|---|
(IU/L) | (mg/dL) | (g/dL) | (IU/L) | (mg/dL) | (mg/dL) | (g/dL) | |
Normal | 820.4 ± 127.98 a | 41.23 ± 28.94 a,b,c,d | 2.24 ± 0.13 | 483.6 ± 119.02 a | 0.14 ± 0.01 a,b | 0.14 ± 0.01 a | 5.32 ± 0.31 |
Alcohol | 1162.64 ± 904.36 a,b | 32.97 ± 10.96 a,b | 2.38 ± 0.08 | 633.73 ± 91.97 b | 0.15 ± 0.01 a,b | 0.15 ± 0.01 b,c | 5.44 ± 0.14 |
Silymarin | 646.7 ± 340.96 a | 26.2 ± 14.27 a | 2.37 ± 0.13 | 555.63 ± 108.75 a,b | 0.14 ± 0.02 a | 0.14 ± 0.02 c | 5.24 ± 0.28 |
pPEM (L) | 695.97 ± 220.62 a | 39.44 ± 22.68 a,b,c | 2.33 ± 0.22 | 552.89 ± 94.56 a,b | 0.15 ± 0.02 a,b | 0.15 ± 0.02 a,b,c | 5.19 ± 0.46 |
pPEM (M) | 818.59 ± 292.64 a | 68.21 ± 26.9 d,e | 2.34 ± 0.18 | 490.23 ± 82.2 a | 0.14 ± 0.01 a | 0.14 ± 0.01 c | 5.22 ± 0.34 |
pPEM (H) | 783.81 ± 174.14 a | 64.56 ± 28.05 c,d,e | 2.39 ± 0.12 | 568.11 ± 85.03 a,b | 0.14 ± 0.02 a,b | 0.14 ± 0.02 a,b,c | 5.32 ± 0.27 |
Lesions/Groups | Normal | Alcohol | Silymarin | pPEM (L) | pPEM (M) | pPEM (H) |
---|---|---|---|---|---|---|
Lobular inflammation | 0 | 2 | 0 | 1 | 0 | 0 |
Fatty degeneration | 0 | 2 | 1 | 1 | 1 | 1 |
Steatosis | 0 | 1 | 0 | 0 | 0 | 0 |
Total score | 0 | 5 | 1 | 2 | 1 | 1 |
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Kim, S.-M.; Diao, W.-J.; An, W.; Kim, H.-J.; Lim, H.-J.; Kim, K.-N.; Bae, G.-W.; Kang, J.-S. Effect of Porcine Placental Extract Mixture on Alcohol-Induced Hepatotoxicity in Rats. Curr. Issues Mol. Biol. 2022, 44, 2029-2037. https://doi.org/10.3390/cimb44050137
Kim S-M, Diao W-J, An W, Kim H-J, Lim H-J, Kim K-N, Bae G-W, Kang J-S. Effect of Porcine Placental Extract Mixture on Alcohol-Induced Hepatotoxicity in Rats. Current Issues in Molecular Biology. 2022; 44(5):2029-2037. https://doi.org/10.3390/cimb44050137
Chicago/Turabian StyleKim, Se-Mi, Wen-Jing Diao, Wen An, Hyun-Jin Kim, Ha-Jong Lim, Keun-Nam Kim, Gun-Won Bae, and Ju-Seop Kang. 2022. "Effect of Porcine Placental Extract Mixture on Alcohol-Induced Hepatotoxicity in Rats" Current Issues in Molecular Biology 44, no. 5: 2029-2037. https://doi.org/10.3390/cimb44050137