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Brief Report

Molecular Profiling of Tumor Tissue in Mexican Patients with Colorectal Cancer

by
Beatriz Armida Flores-López
1,
María de la Luz Ayala-Madrigal
1,
José Miguel Moreno-Ortiz
1,
Jorge Peregrina-Sandoval
2,
Miguel Ángel Trujillo-Rojas
1,
José Luis Venegas-Rodríguez
1,
Rosario Hernández-Ramírez
1,
Martha Alejandra Fernández-Galindo
1 and
Melva Gutiérrez-Angulo
1,3,*
1
Departamento de Biología Molecular y Genómica, Doctorado en Genética Humana e Instituto de Genética Humana “Dr. Enrique Corona Rivera”, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico
2
Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Guadalajara 45200, Jalisco, Mexico
3
Departamento de Ciencias de la Salud, Centro Universitario de los Altos, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2022, 44(8), 3770-3778; https://doi.org/10.3390/cimb44080258
Submission received: 13 July 2022 / Revised: 11 August 2022 / Accepted: 17 August 2022 / Published: 20 August 2022
(This article belongs to the Special Issue Advances in Molecular Pathogenesis Regulation in Cancer)
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Abstract

Colorectal cancer is a heterogeneous disease with multiple genomic changes that influence the clinical management of patients; thus, the search for new molecular targets remains necessary. The aim of this study was to identify genetic variants in tumor tissues from Mexican patients with colorectal cancer, using massive parallel sequencing. A total of 4813 genes were analyzed in tumoral DNA from colorectal cancer patients, using the TruSight One Sequencing panel. From these, 192 variants with clinical associations were found distributed in 168 different genes, of which 46 variants had not been previous reported in the literature or databases, although genes harboring those variants had already been described in colorectal cancer. Enrichment analysis of the affected genes was performed using Reactome software; pathway over-representation showed significance for disease, signal transduction, and immune system subsets in all patients, while exclusive subsets such as DNA repair, autophagy, and RNA metabolism were also found. Those characteristics, whether individual or shared, could give tumors specific capabilities for survival, aggressiveness, or response to treatment. Our results can be useful for future investigations targeting specific characteristics of tumors in colorectal cancer patients. The identification of exclusive or common pathways in colorectal cancer patients could be important for better diagnosis and personalized cancer treatment.
Keywords: colorectal cancer; massive parallel sequencing; pathogenic variant; likely pathogenic variant; somatic variants; exome sequencing; genetics; molecular pathways colorectal cancer; massive parallel sequencing; pathogenic variant; likely pathogenic variant; somatic variants; exome sequencing; genetics; molecular pathways

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MDPI and ACS Style

Flores-López, B.A.; Ayala-Madrigal, M.d.l.L.; Moreno-Ortiz, J.M.; Peregrina-Sandoval, J.; Trujillo-Rojas, M.Á.; Venegas-Rodríguez, J.L.; Hernández-Ramírez, R.; Fernández-Galindo, M.A.; Gutiérrez-Angulo, M. Molecular Profiling of Tumor Tissue in Mexican Patients with Colorectal Cancer. Curr. Issues Mol. Biol. 2022, 44, 3770-3778. https://doi.org/10.3390/cimb44080258

AMA Style

Flores-López BA, Ayala-Madrigal MdlL, Moreno-Ortiz JM, Peregrina-Sandoval J, Trujillo-Rojas MÁ, Venegas-Rodríguez JL, Hernández-Ramírez R, Fernández-Galindo MA, Gutiérrez-Angulo M. Molecular Profiling of Tumor Tissue in Mexican Patients with Colorectal Cancer. Current Issues in Molecular Biology. 2022; 44(8):3770-3778. https://doi.org/10.3390/cimb44080258

Chicago/Turabian Style

Flores-López, Beatriz Armida, María de la Luz Ayala-Madrigal, José Miguel Moreno-Ortiz, Jorge Peregrina-Sandoval, Miguel Ángel Trujillo-Rojas, José Luis Venegas-Rodríguez, Rosario Hernández-Ramírez, Martha Alejandra Fernández-Galindo, and Melva Gutiérrez-Angulo. 2022. "Molecular Profiling of Tumor Tissue in Mexican Patients with Colorectal Cancer" Current Issues in Molecular Biology 44, no. 8: 3770-3778. https://doi.org/10.3390/cimb44080258

APA Style

Flores-López, B. A., Ayala-Madrigal, M. d. l. L., Moreno-Ortiz, J. M., Peregrina-Sandoval, J., Trujillo-Rojas, M. Á., Venegas-Rodríguez, J. L., Hernández-Ramírez, R., Fernández-Galindo, M. A., & Gutiérrez-Angulo, M. (2022). Molecular Profiling of Tumor Tissue in Mexican Patients with Colorectal Cancer. Current Issues in Molecular Biology, 44(8), 3770-3778. https://doi.org/10.3390/cimb44080258

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